The Arabidopsis protein NPF6.2/NRT1.4 is a plasma membrane nitrate transporter and a target of protein kinase CIPK23

Corratgé-Faillie, Claire; Raddatz, Natalia; Mendoza, Irene; Lindahl, Marika; Angeli, Alexis De; Lacombe, Benoı̂t; Quintero, Francisco J.; Pardo, José M.

doi:10.1016/j.plaphy.2021.10.016

Cited by 16 publications

(7 citation statements)

References 55 publications

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“…For example, CIPK23‐CBL1/9 complexes have been widely shown to enhance root K + uptake via AKT1‐like and HAK5‐like transport systems (Xu et al ., 2006; Li et al ., 2014; Ragel et al ., 2015; Amo et al ., 2021; Han et al ., 2021). They also regulate the Arabidopsis NRT1.4 transporter, which contributes to root/shoot distribution of K + (Morales de los et al ., 2021). By contrast, no reports on the regulation of xylem K + load mechanisms by CIPK‐CBLs are available.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of SlSKOR by SlCIPK23‐SlCBL1/9 uncovers CIPK‐CBL‐target network rewiring in land plants

et al. 2023

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Transport of K + to the xylem is a key process in the mineral nutrition of the shoots. Although CIPK-CBL complexes have been widely shown to regulate K + uptake transport systems, no information is available about the xylem ones. Here, we studied the physiological roles of the voltage-gated K + channel SlSKOR and its regulation by the SlCIPK23-SlCBL1/9 complexes in tomato plants.We phenotyped gene-edited slskor and slcipk23 tomato knockout mutants and carried out two-electrode voltage-clamp (TEVC) and BiFC assays in Xenopus oocytes as key approaches.SlSKOR was preferentially expressed in the root stele and was important not only for K + transport to shoots but also, indirectly, for that of Ca 2+ , Mg 2+ , Na + , NO 3 − , and Cl − . Surprisingly, the SlCIPK23-SlCBL1/9 complexes turned out to be negative regulators of SlSKOR. Inhibition of SlSKOR by SlCIPK23-SlCBL1/9 was observed in Xenopus oocytes and tomato plants. Regulation of SKOR-like channels by CIPK23-CBL1 complexes was also present in Medicago, grapevine, and lettuce but not in Arabidopsis and saltwater cress.Our results provide a molecular framework for coordinating root K + uptake and its translocation to the shoot by SlCIPK23-SlCBL1/9 in tomato plants. Moreover, they evidenced that CIPK-CBL-target networks have evolved differently in land plants.

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Section: Introductionmentioning

confidence: 99%

Inhibition of SlSKOR by SlCIPK23‐SlCBL1/9 uncovers CIPK‐CBL‐target network rewiring in land plants

et al. 2023

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“…These properties are crucial for cytosolic sodium homeostasis at high external pH values (Ramos et al, 2011). Functional complementation of the disrupted strain Δ ynt1 had been successfully achieved for the expression of the Arabidopsis dual‐affinity nitrate transporter CHL1/AtNRT1.1/AtNPF6.3 (Martín et al, 2008) and recently for the plasma membrane nitrate transporter AtNPF6.2/NRT1.4 (Morales de los Ríos et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Identity and functional characterisation of the transporter supporting the Na⁺‐dependent high‐affinity NO₃⁻ uptake in Zostera marina L.

Rubio

Díaz‐García

Martín‐Pizarro

et al. 2023

Plant Cell & Environment

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Zostera marina is a seagrass, a group of angiosperms that evolved from land to live submerged in seawater, an environment of high salinity, alkaline pH and usually very low NO3−. In 2000, we reported the first physiological evidence for the Na+‐dependent high‐affinity NO3− uptake in this plant. Now, to determine the molecular identity of this process, we searched for NO3− transporters common to other vascular plants encoded in Z. marina's genome. We cloned two candidates, ZosmaNPF6.3 and ZosmaNRT2 with its partner protein ZosmaNAR2. ZosmaNAR2 expression levels increase up to 4.5‐fold in Z. marina leaves under NO3−‐deficiency, while ZosmaNRT2 and ZosmaNPF6.3 expressions were low and unaffected by NO3−. NO3− transport capacity, kinetic properties and H+ or Na+‐dependence were examined by heterologous expression in the Hansenula polymorpha high‐affinity NO3− transporter gene disrupted strain (∆ynt1). ZosmaNPF6.3 functions as a H+‐dependent NO3− transporter, without functionality at alkaline pH and apparent dual kinetics (KM = 11.1 µM at NO3− concentrations below 50 µM). ZosmaNRT2 transports NO3− in a H+‐independent but Na+‐dependent manner (KM = 1 mM Na+), with low NO3− affinity (KM = 30 µM). When ZosmaNRT2 and ZosmaNAR2 are co‐expressed, a Na+‐dependent high‐affinity NO3− transport occurs (KM = 5.7 µM NO3−), mimicking the in vivo value. These results are discussed in the physiological context, providing evidence that ZosmaNRT2 is a Na+‐dependent high‐affinity NO3− transporter, the first of its kind to be functionally characterised in a vascular plant, that requires ZosmaNAR2 to achieve the necessary high‐affinity for nitrate uptake from seawater.

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“…K has high mobility in plants, and K cycling between roots and shoots also plays an important role in the transport of NO 3 – and amino acids in the xylem and phloem ( Coskun et al, 2017b ). It is well known that the uptake and transport of NO 3 – is largely determined by nitrate transporters (NRT) and the NO 3 – can be further transported and utilized by the catalysis of a series of N-metabolizing enzymes ( Teng et al, 2017 ; Morales de Los Rios et al, 2021 ). K promoted the activities of nitrate reductase (NR), glutamine synthetase (GS), glutamate synthase (GOGAT) and other nitrogen metabolism enzymes, which were verified in cotton ( Hu et al, 2016 ) and cucumber ( Ruiz and Romero, 2002 ).…”

Section: Introductionmentioning

confidence: 99%

Appropriate and Constant Potassium Supply Promotes the Growth of M9T337 Apple Rootstocks by Regulating Endogenous Hormones and Carbon and Nitrogen Metabolism

Wang

Xing

et al. 2022

Front. Plant Sci.

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Potassium (K) is an indispensable nutrient element in the development of fruit trees in terms of yield and quality. It is unclear how a stable or unstable supply of K affects plant growth. We studied the root morphology and physiological and molecular changes in the carbon and nitrogen metabolism of M9T337 apple rootstock under different K levels and supply methods using hydroponics. Five K supply treatments were implemented: continuous low K (KL), initial low and then high K (KLH), appropriate and constant K (KAC), initial high and then low K (KHL), and continuous high K (KH). The results showed that the biomass, root activity, photosynthesis, and carbon and nitrogen metabolism of the M9T337 rootstocks were inhibited under KL, KH, KLH and KHL conditions. The KAC treatment promoted root growth by optimizing endogenous hormone content, enhancing carbon and nitrogen metabolism enzyme activities, improving photosynthesis, optimizing the distribution of carbon and nitrogen, and upregulating the transcription levels of nitrogen assimilation-related genes (nitrate reductase, glutamine synthetase, glutamate synthase, MdNRT1.1, MdNRT1.2, MdNRT1.5, MdNRT2.4). These results suggest that an appropriate and constant K supply ensures the efficient assimilation and utilization of nitrogen and carbon.

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The Arabidopsis protein NPF6.2/NRT1.4 is a plasma membrane nitrate transporter and a target of protein kinase CIPK23

Abstract: The Arabidopsis protein NPF6.2/NRT1.4 is a plasma membrane nitrate transporter and a target of protein kinase CIPK23

Cited by 16 publications

References 55 publications

Inhibition of SlSKOR by SlCIPK23‐SlCBL1/9 uncovers CIPK‐CBL‐target network rewiring in land plants

Inhibition of SlSKOR by SlCIPK23‐SlCBL1/9 uncovers CIPK‐CBL‐target network rewiring in land plants

Identity and functional characterisation of the transporter supporting the Na⁺‐dependent high‐affinity NO₃⁻ uptake in Zostera marina L.

Appropriate and Constant Potassium Supply Promotes the Growth of M9T337 Apple Rootstocks by Regulating Endogenous Hormones and Carbon and Nitrogen Metabolism

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The Arabidopsis protein NPF6.2/NRT1.4 is a plasma membrane nitrate transporter and a target of protein kinase CIPK23

Abstract: The Arabidopsis protein NPF6.2/NRT1.4 is a plasma membrane nitrate transporter and a target of protein kinase CIPK23

Cited by 16 publications

References 55 publications

Inhibition of SlSKOR by SlCIPK23‐SlCBL1/9 uncovers CIPK‐CBL‐target network rewiring in land plants

Inhibition of SlSKOR by SlCIPK23‐SlCBL1/9 uncovers CIPK‐CBL‐target network rewiring in land plants

Identity and functional characterisation of the transporter supporting the Na+‐dependent high‐affinity NO3− uptake in Zostera marina L.

Appropriate and Constant Potassium Supply Promotes the Growth of M9T337 Apple Rootstocks by Regulating Endogenous Hormones and Carbon and Nitrogen Metabolism

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Identity and functional characterisation of the transporter supporting the Na⁺‐dependent high‐affinity NO₃⁻ uptake in Zostera marina L.