The Arabidopsis TETRATRICOPEPTIDE THIOREDOXIN-LIKE Gene Family Is Required for Osmotic Stress Tolerance and Male Sporogenesis

Lakhssassi, Naoufal; Doblas, Verónica G.; Rosado, Abel; Valle, Alicia Esteban del; Posé, David; Jiménez, Antonio J.; Castillo, Araceli G.; Valpuesta, Victoriano; Borsani, Omar; Botella, Miguel A.

doi:10.1104/pp.111.188920

Cited by 55 publications

(68 citation statements)

References 49 publications

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“…It is a gene proven to be involved in the resistance of soybean to SCN Lakhssassi et al, 2012;. This study confirmed those earlier findings and showed the complexity of the introgressed region.…”

supporting

confidence: 80%

Additional Polymorphisms Linked to Soybean Cyst Nematode Resistance At The Rhg4 Locus

Zatserklyana

Meksem

Lightfoot

2017

AJB

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Glycine max L. Merr., (soybean) is one of the major crops in the United States, south America and Asia. Yet, the seed yield of soybean is significantly reduced due to Heterodera glycines (Ichinohe), the soybean cyst nematode (SCN). SCN is one of the most destructive pests and pathogens of soybean because the main methods for control have proven difficult. Lineages of soybean that are resistant to SCN have been developed but it is yet unknown what all the molecular causes of this resistance are. Rhg1 and Rhg4 loci are two of about 10 loci that underlie resistance. The Rhg4 locus, is required for resistance to SCN race 3 in 'Peking'-type derived resistances. One gene in the locus has been isolated and a causative link shown, but the surrounding regions have not been fully analyzed. Focusing on the Rhg4 locus, this study was aimed at uncovering other potential causes of resistance of soybean to SCN. In a bioinformatic analysis of the Rhg4 locus, alleles of nine genes were analyzed. The set of large intergenic regions have key regulatory elements in them. Since partial resistances are often multigeneic, some of these nine genes could be candidates for causing and regulating resistance.

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supporting

confidence: 80%

Additional Polymorphisms Linked to Soybean Cyst Nematode Resistance At The Rhg4 Locus

Zatserklyana

Meksem

Lightfoot

2017

AJB

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“…Previous studies in the plant model Arabidopsis thaliana have shown that a TPR protein family named Tetratricopeptide repeat thioredoxin-like (TTL), formed by four members. TTL1, TTL3, and TTL4, but not TTL2, presented an additive effect in response to osmotic stress tolerance and are essential for root growth and integrity, while all present the same amino acid sequence length with high identity12. This is an example of a duplicated gene resulting in a truncated protein (50 aa less) that presents an additive effect in resistance to a pathogen.…”

Section: Discussionmentioning

confidence: 99%

“…Importantly, the TPR domain facilitates specific interactions with a partner protein(s)11. Moreover, TPR domains also play important roles in aspects of plant development; being essential for gametophytic viability as well as root growth and integrity under osmotic stress12. In addition, a different member of TPR containing proteins, TTL3 , was found to interact with the constitutively active VH1/BRL2, a protein homologous to the brassinosteroid receptor BRI1, and play a role in vasculature development13.…”

mentioning

confidence: 99%

Characterization of the Soluble NSF Attachment Protein gene family identifies two members involved in additive resistance to a plant pathogen

Lakhssassi

Liu

Bekal

et al. 2017

Sci Rep

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113

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Proteins with Tetratricopeptide-repeat (TPR) domains are encoded by large gene families and distributed in all plant lineages. In this study, the Soluble NSF-Attachment Protein (SNAP) subfamily of TPR containing proteins is characterized. In soybean, five members constitute the SNAP gene family: GmSNAP18, GmSNAP11, GmSNAP14, GmSNAP02, and GmSNAP09. Recently, GmSNAP18 has been reported to mediate resistance to soybean cyst nematode (SCN). Using a population of recombinant inbred lines from resistant and susceptible parents, the divergence of the SNAP gene family is analysed over time. Phylogenetic analysis of SNAP genes from 22 diverse plant species showed that SNAPs were distributed in six monophyletic clades corresponding to the major plant lineages. Conservation of the four TPR motifs in all species, including ancestral lineages, supports the hypothesis that SNAPs were duplicated and derived from a common ancestor and unique gene still present in chlorophytic algae. Syntenic analysis of regions harbouring GmSNAP genes in soybean reveals that this family expanded from segmental and tandem duplications following a tetraploidization event. qRT-PCR analysis of GmSNAPs indicates a co-regulation following SCN infection. Finally, genetic analysis demonstrates that GmSNAP11 contributes to an additive resistance to SCN. Thus, GmSNAP11 is identified as a novel minor gene conferring resistance to SCN.

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“…Within this dataset, we identified four transcription factors including soybean homologues of AtBZIP9, AtTTL1, AtIAA17 and AtIDD2. While roles in defence have not been demonstrated for AtBZIP9 and AtIDD2, AtTTL1 is required for osmotic stress tolerance (Lakhssassi et al 2012) and AtIAA17 increases tolerance to Plasmodiophora brassicae (Kazan and Manners 2009). …”

Section: The Role Of Transcription Factors In Mediating Defence Respomentioning

confidence: 99%

Transcriptome analyses and virus induced gene silencing identify genes in the Rpp4-mediated Asian soybean rust resistance pathway

Morales

O’Rourke

Mortel

et al. 2013

Functional Plant Biol.

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Rpp4 (Resistance to Phakopsora pachyrhizi 4) confers resistance to Phakopsora pachyrhizi Sydow, the causal agent of Asian soybean rust (ASR). By combining expression profiling and virus induced gene silencing (VIGS), we are developing a genetic framework for Rpp4-mediated resistance. We measured gene expression in mock-inoculated and P. pachyrhizi-infected leaves of resistant soybean accession PI459025B (Rpp4) and the susceptible cultivar (Williams 82) across a 12-day time course. Unexpectedly, two biphasic responses were identified. In the incompatible reaction, genes induced at 12 h after infection (hai) were not differentially expressed at 24 hai, but were induced at 72 hai. In contrast, genes repressed at 12 hai were not differentially expressed from 24 to 144 hai, but were repressed 216 hai and later. To differentiate between basal and resistance-gene (R-gene) mediated defence responses, we compared gene expression in Rpp4-silenced and empty vector-treated PI459025B plants 14 days after infection (dai) with P. pachyrhizi. This identified genes, including transcription factors, whose differential expression is dependent upon Rpp4. To identify differentially expressed genes conserved across multiple P. pachyrhizi resistance pathways, Rpp4 expression datasets were compared with microarray data previously generated for Rpp2 and Rpp3-mediated defence responses. Fourteen transcription factors common to all resistant and susceptible responses were identified, as well as fourteen transcription factors unique to R-gene-mediated resistance responses. These genes are targets for future P. pachyrhizi resistance research. Abstract. Rpp4 (Resistance to Phakopsora pachyrhizi 4) confers resistance to Phakopsora pachyrhizi Sydow, the causal agent of Asian soybean rust (ASR). By combining expression profiling and virus induced gene silencing (VIGS), we are developing a genetic framework for Rpp4-mediated resistance. We measured gene expression in mock-inoculated and P. pachyrhizi-infected leaves of resistant soybean accession PI459025B (Rpp4) and the susceptible cultivar (Williams 82) across a 12-day time course. Unexpectedly, two biphasic responses were identified. In the incompatible reaction, genes induced at 12 h after infection (hai) were not differentially expressed at 24 hai, but were induced at 72 hai. In contrast, genes repressed at 12 hai were not differentially expressed from 24 to 144 hai, but were repressed 216 hai and later. To differentiate between basal and resistance-gene (R-gene) mediated defence responses, we compared gene expression in Rpp4-silenced and empty vector-treated PI459025B plants 14 days after infection (dai) with P. pachyrhizi. This identified genes, including transcription factors, whose differential expression is dependent upon Rpp4. To identify differentially expressed genes conserved across multiple P. pachyrhizi resistance pathways, Rpp4 expression datasets were compared with microarray data previously generated for Rpp2 and Rpp3-mediated defence responses. Fourteen transcription...

show abstract

The Arabidopsis TETRATRICOPEPTIDE THIOREDOXIN-LIKE Gene Family Is Required for Osmotic Stress Tolerance and Male Sporogenesis

Cited by 55 publications

References 49 publications

Additional Polymorphisms Linked to Soybean Cyst Nematode Resistance At The Rhg4 Locus

Additional Polymorphisms Linked to Soybean Cyst Nematode Resistance At The Rhg4 Locus

Characterization of the Soluble NSF Attachment Protein gene family identifies two members involved in additive resistance to a plant pathogen

Transcriptome analyses and virus induced gene silencing identify genes in the Rpp4-mediated Asian soybean rust resistance pathway

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