The Arabinogalactan Protein Family of Centaurium erythraea Rafn

Paunović, Danijela M.; Ćuković, Katarina; Bogdanović﻿﻿﻿, Milica; Todorović, Slađana; Trifunović‐Momčilov, Milana; Subotić, Angelina; Simonović, Ana; Dragićević, Milan

doi:10.3390/plants10091870

Cited by 4 publications

(4 citation statements)

References 79 publications

(174 reference statements)

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“…Chimeric AGP sequences were identified by detecting (STAGV)P by using the implementation in the “ragp”‐package with the strict mode (at least four dipeptides in a maximal distance of four amino acids) used by Paunović et al. (2021). All dipeptides in an annotated pfam domain (by querying the Pfam database via the CDD webtool, Lu et al., 2020) or in the signal sequence were not count.…”

Section: Methodsmentioning

confidence: 99%

Fern cell walls and the evolution of arabinogalactan proteins in streptophytes

et al. 2023

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Significant changes have occurred in plant cell wall composition during evolution and diversification of tracheophytes. As the sister lineage to seed plants, knowledge on the cell wall of ferns is key to track evolutionary changes across tracheophytes and to understand seed plant-specific evolutionary innovations. Fern cell wall composition is not fully understood, including limited knowledge of glycoproteins such as the fern arabinogalactan proteins (AGPs). Here, we characterize the AGPs from the leptosporangiate fern genera Azolla, Salvinia, and Ceratopteris. The carbohydrate moiety of seed plant AGPs consists of a galactan backbone including mainly 1,3-and 1,3,6-linked pyranosidic galactose, which is conserved across the investigated fern AGPs. Yet, unlike AGPs of angiosperms, those of ferns contained the unusual sugar 3-Omethylrhamnose. Besides terminal furanosidic arabinose, Ara (Araf), the main linkage type of Araf in the ferns was 1,2-linked Araf, whereas in seed plants 1,5-linked Araf is often dominating. Antibodies directed against carbohydrate epitopes of AGPs supported the structural differences between AGPs of ferns and seed plants. Comparison of AGP linkage types across the streptophyte lineage showed that angiosperms have rather conserved monosaccharide linkage types; by contrast bryophytes, ferns, and gymnosperms showed more variability. Phylogenetic analyses of glycosyltransferases involved in AGP biosynthesis and bioinformatic search for AGP protein backbones revealed a versatile genetic toolkit for AGP complexity in ferns. Our data reveal important differences across AGP diversity of which the functional significance is unknown. This diversity sheds light on the evolution of the hallmark feature of tracheophytes: their elaborate cell walls.

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Section: Methodsmentioning

confidence: 99%

Fern cell walls and the evolution of arabinogalactan proteins in streptophytes

et al. 2023

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“…Chimeric AGP sequences were identified by detecting (STAGV)P by using the implementation in the “ragp”-package with the strict mode (at least 4 dipeptided in a maximal distance of 4 amino acids) used by Paunović et al . (2021).…”

Section: Methodsmentioning

confidence: 99%

“…Chimeric AGP sequences were identified by detecting (STAGV)P by using the implementation in the "ragp"-package with the strict mode (at least 4 dipeptided in a maximal distance of 4 amino acids) used by Paunović et al (2021). All dipeptides in an annotated pfam domain (by querying the Pfam database via the CDD webtool, Lu et al, 2020) or in the signal sequence were not count.…”

Section: Agp Backbone Sequence Identification and Classificationmentioning

confidence: 99%

Fern cell walls and the evolution of arabinogalactan-proteins in streptophytes

Mueller

Pfeifer

Schuldt

et al. 2022

Preprint

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Significant changes have occurred in plant cell wall composition during evolution and diversification of tracheophytes. As the sister lineage to seed plants, knowledge on the cell wall of ferns is key to track evolutionary changes across tracheophytes and to understand seed plant-specific evolutionary innovations. Fern cell wall composition is not fully understood, including limited knowledge of glycoproteins such as the fern arabinogalactan-proteins (AGPs). Here, we characterize the AGPs from the leptosporangiate fern genera Azolla, Salvinia and Ceratopteris. The carbohydrate moiety of seed plant AGPs consists of a galactan backbone including mainly 1,3- and 1,3,6-linked pyranosidic galactose, which is conserved across the investigated fern AGPs. Yet, unlike AGPs of angiosperms, those of ferns contained the unusual sugar 3-O-methylrhamnose. Besides terminal furanosidic Ara (Araf), the main linkage type of Araf in the ferns was 1,2-linked Araf, whereas in seed plants 1,5-linked Araf is often dominating. Antibodies directed against carbohydrate epitopes of AGPs supported the structural differences between AGPs of ferns and seed plants. Comparison of AGP linkage types across the streptophyte lineage showed that angiosperms have rather conserved monosaccharide linkage types; by contrast bryophytes, ferns and gymnosperms showed more variability. Phylogenetic analyses of glycosyltransferases involved in AGP biosynthesis and bioinformatic search for AGP protein backbones revealed a versatile genetic toolkit for AGP complexity in ferns. Our data reveal important differences across AGP diversity which functional significance is unknown. This diversity sheds light on the evolution of the hallmark feature of tracheophytes: their elaborate cell walls.

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“…Lyngb. [ 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 ]. Several excellent reviews have summarized that AGPs are associated with vegetative growth, reproductive development, tissue regeneration, stress response, and other vital activities in plants [ 2 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

Arabinogalactan Proteins: Focus on the Role in Cellulose Synthesis and Deposition during Plant Cell Wall Biogenesis

Lin

Miao

Huang

et al. 2022

IJMS

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Arabinogalactan proteins (AGPs) belong to a family of glycoproteins that are widely present in plants. AGPs are mostly composed of a protein backbone decorated with complex carbohydrate side chains and are usually anchored to the plasma membrane or secreted extracellularly. A trickle of compelling biochemical and genetic evidence has demonstrated that AGPs make exciting candidates for a multitude of vital activities related to plant growth and development. However, because of the diversity of AGPs, functional redundancy of AGP family members, and blunt-force research tools, the precise functions of AGPs and their mechanisms of action remain elusive. In this review, we put together the current knowledge about the characteristics, classification, and identification of AGPs and make a summary of the biological functions of AGPs in multiple phases of plant reproduction and developmental processes. In addition, we especially discuss deeply the potential mechanisms for AGP action in different biological processes via their impacts on cellulose synthesis and deposition based on previous studies. Particularly, five hypothetical models that may explain the AGP involvement in cellulose synthesis and deposition during plant cell wall biogenesis are proposed. AGPs open a new avenue for understanding cellulose synthesis and deposition in plants.

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The Arabinogalactan Protein Family of Centaurium erythraea Rafn

Cited by 4 publications

References 79 publications

Fern cell walls and the evolution of arabinogalactan proteins in streptophytes

Fern cell walls and the evolution of arabinogalactan proteins in streptophytes

Fern cell walls and the evolution of arabinogalactan-proteins in streptophytes

Arabinogalactan Proteins: Focus on the Role in Cellulose Synthesis and Deposition during Plant Cell Wall Biogenesis

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