The archaeal triphosphate tunnel metalloenzyme SaTTM defines structural determinants for the diverse activities in the CYTH protein family

Abstract

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Putative Nucleotide-Based Second Messengers in the Archaeal Model Organisms Haloferax volcanii and Sulfolobus acidocaldarius

“…H. volcanii as well as S. acidocaldarius each contain a single gene encoding for a putative class IV AC ( HVO _1648 and Saci _0718). The S. acidocaldarius gene product of Saci _0718 has recently, however, been demonstrated not to function as a cyclase but as a phosphohydrolase of the triphosphate tunnel metalloenzyme (TTM) family ( Vogt et al, 2021 ). An in this context performed systematic sequence similarity network analysis of the CYTH superfamily unveiled that actual class IV ACs only account for a small subgroup, which is entirely of bacterial origin ( Vogt et al, 2021 ).…”

“…The S. acidocaldarius gene product of Saci _0718 has recently, however, been demonstrated not to function as a cyclase but as a phosphohydrolase of the triphosphate tunnel metalloenzyme (TTM) family ( Vogt et al, 2021 ). An in this context performed systematic sequence similarity network analysis of the CYTH superfamily unveiled that actual class IV ACs only account for a small subgroup, which is entirely of bacterial origin ( Vogt et al, 2021 ). This is in accordance with the observation that a H. volcanii deletion mutant lacking HVO _1648 has unchanged 3′,5′-cAMP levels (preliminary data).…”

Update on Thiamine Triphosphorylated Derivatives and Metabolizing Enzymatic Complexes

“…Surprisingly, both have a higher activity towards PPi than towards triphosphorylated substrates and both carry a uridine kinase domain in addition to the CYTH domain. The only archaeal TTM protein characterized enzymatically proved to be a tripolyphosphatase [ 82 ].…”

Data_Sheet_1.PDF

“…H. volcanii as well as S. acidocaldarius each contain a single gene encoding for a putative class IV AC (HVO_1648 and Saci_0718). The S. acidocaldarius gene product of Saci_0718 has recently, however, been demonstrated not to function as a cyclase but as a phosphohydrolase of the triphosphate tunnel metalloenzyme (TTM) family (Vogt et al, 2021). An in this context performed systematic sequence similarity network analysis of the CYTH superfamily unveiled that actual class IV ACs only account for a small subgroup, which is entirely of bacterial origin (Vogt et al, 2021).…”

“…The S. acidocaldarius gene product of Saci_0718 has recently, however, been demonstrated not to function as a cyclase but as a phosphohydrolase of the triphosphate tunnel metalloenzyme (TTM) family (Vogt et al, 2021). An in this context performed systematic sequence similarity network analysis of the CYTH superfamily unveiled that actual class IV ACs only account for a small subgroup, which is entirely of bacterial origin (Vogt et al, 2021). This is in accordance with the observation that a H. volcanii deletion mutant lacking HVO_1648 has unchanged 3 ,5 -cAMP levels (preliminary data).…”