The Central American isthmus was a major dispersal route for plant taxa originally brought under cultivation in the domestication centers of southern Mexico and northern South America. Recently developed methodologies in the archaeological and biological sciences are providing increasing amounts of data regarding the timing and nature of these dispersals and the associated transition to food production in various regions. One of these methodologies, starch grain analysis, recovers identifiable microfossils of economic plants directly off the stone tools used to process them. We report on new starch grain evidence from Panama demonstrating the early spread of three important New World cultigens: maize (Zea mays), manioc (Manihot esculenta), and arrowroot (Maranta arundinacea). Maize starch recovered from stone tools at a site located in the Pacific lowlands of central Panama confirms previous archaeobotanical evidence for the use of maize there by 7800 -7000 cal BP. Starch evidence from preceramic sites in the less seasonal, humid premontane forests of Chiriquí province, western Panama, shows that maize and root crops were present by 7400 -5600 cal BP, several millennia earlier than previously documented. Several local starchy resources, including Zamia and Dioscorea spp., were also used. The data from both regions suggest that crop dispersals took place via diffusion or exchange of plant germplasm rather than movement of human populations practicing agriculture.agricultural origins ͉ crop dispersals ͉ Neotropics ͉ starch grain analysis W ith the advent of molecular studies directed toward understanding the phylogenetics of various economic plants throughout the world, the domestication hearths of several major crop plants have been identified (e.g., refs. 2-5). However, tracing the dispersal of these domesticates, and the economic transition from foraging to food production by the societies that domesticated or adopted them, remains firmly dependent on the recovery of identifiable archaeobotanical remains. The development and application of microbotanical techniques in archaeology has led to major advances in investigating plant use and subsistence in regions where preservation of macrobotanical remains (seeds, fruits, tubers) is poor. In the Americas, phytoliths, pollen, and most recently starch grains, have provided substantial empirical evidence demonstrating the considerable antiquity of food production and crop dispersals in tropical regions once considered peripheral to agricultural origins. Numerous studies now show that people were experimenting with horticulture and moving domesticated plants around tropical forests by 9500-7500 cal BP, ¶ and that food production concentrating on a few particularly productive cultigens was widespread throughout the Neotropics by 5500 cal BP (6-14).The Isthmus of Panama forms a relatively narrow landbridge between North and South America, and was the terrestrial route for the dispersal of numerous domesticates. Not surprisingly, some of the earliest evidence for the spre...