The ARM repeat domain of hemocyanin interacts with MKK4 to modulate antimicrobial peptides expression

Aweya, Jude Juventus; Zhuang, Kaiying; Liu, Yiqi; Fan, Jiaohong; Yao, Defu; Wang, Fan; Chen, Xiaohan; Li, Shengkang; Ma, Hongyu; Zhang, Yueling

doi:10.1016/j.isci.2022.103958

Cited by 15 publications

(6 citation statements)

References 72 publications

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“…Armadillo repeat-containing protein 4 ( armc4 ) encodes the armadillo repeat domain which is involved in the expression of antimicrobial peptides in the immune system. Interaction of hemocyanin via its ARM repeat domain with MKK4 promoted the transcription of genes expressing antimicrobial peptides 57 . The EF-hand calcium-binding domain-containing protein ( efcab1 ) encodes calcium-responsive proteins which are involved in various cellular functions.…”

Section: Discussionmentioning

confidence: 99%

Differential responses of hepatopancreas transcriptome between fast and slow growth in giant freshwater prawns (Macrobrachium rosenbergii) fed a plant-based diet

Phonsiri,

Mavichak,

Panserat

et al. 2024

Sci Rep

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Efficient utilisation of plant-based diets in the giant freshwater prawn, Marcrobrachium rosenbergii, varies according to individual, suggesting that it might be associated with differences in physiological and metabolic responses. Therefore, we aimed to investigate the individual differences in the growth response of shrimp fed to a soybean-based diet (SBM). Two hundred shrimp were fed SBM for 90 days, and specific growth rate (SGR) was determined individually. Fast- and slow-growing shrimp (F-shrimp vs. S-shrimp), with the highest and lowest 5% SGRs, respectively, were sampled to determine haemolymph chemistry and carcass composition. The hepatopancreas of these shrimps were used for transcriptome analysis through RNA sequencing (RNA-Seq). The results showed no significant differences in haemolymph chemistry parameters. In terms of carcass proximate composition, F-shrimp exhibited higher protein composition than did S-shrimp, suggesting that F-shrimp have higher protein anabolism. Using RNA-seq and real-time reverse transcription polymerase chain reaction (qRT-PCR), the expression levels of several genes encoding physiologic and metabolic enzymes were found to be upregulated in F-shrimp compared to in S-shrimp, suggesting that these enzymes/proteins mediated the efficient use of SBM-based diets for growth promotion in shrimp. Various DEGs associated with the immune system were observed, indicating a difference in immune processes between F- and S-shrimp. The expression of several housekeeping genes was found to be upregulated in S-shrimp. Collectively, the upregulated expression of several enzymes associated with physiological and/or metabolic processes and increased protein anabolism may be attributed to the efficient use of SBM for maximal growth in shrimp.

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Section: Discussionmentioning

confidence: 99%

Differential responses of hepatopancreas transcriptome between fast and slow growth in giant freshwater prawns (Macrobrachium rosenbergii) fed a plant-based diet

Phonsiri,

Mavichak,

Panserat

et al. 2024

Sci Rep

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“…Here, we find that bacteria pathogens induced high levels of hemocyanin in shrimp hepatopancreas, coupled with a concomitant increase in the mRNA transcripts and protein levels of SREBP and key ER stress-related proteins ( Figure 1 ). We contend that a regulatory relationship exists between hemocyanin and ER stress proteins in the hepatopancreas, given that hemocyanin is a multifunctional immune response protein [ 38 ]. Indeed, we find that hemocyanin ( Pv HMC) interacts with ER stress proteins in protein–protein interaction studies, especially with Bip.…”

Section: Discussionmentioning

confidence: 99%

“…Most importantly, Pv SREBP could regulate the expression of the multifunctional respiratory protein hemocyanin [ 32 ] to affect the expression of other lipogenic and immune-related genes through a putative positive feedback mechanism [ 30 ]. Besides the role of hemocyanin in respiration and immune response [ 32 , 33 , 34 ], it plays a vital role in shrimp response to environmental cues, including hypoxia, pathogen stress, oxidative stress, ammonia stress, and thermal stress [ 35 , 36 , 37 , 38 , 39 , 40 ]. Given that hemocyanin is involved in shrimp response to various stressors and our recent finding that a putative positive feedback relationship exists between hemocyanin and SREBP, we wondered whether hemocyanin modulates ER stress to affect SREBP expression and fatty acid levels in penaeid shrimp.…”

Section: Introductionmentioning

confidence: 99%

Modulation of SREBP Expression and Fatty Acid Levels by Bacteria-Induced ER Stress Is Mediated by Hemocyanin in Penaeid Shrimp

et al. 2023

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Many environmental and pathogenic insults induce endoplasmic reticulum (ER) stress in animals, especially in aquatic ecosystems, where these factors are crucial for life. In penaeid shrimp, pathogens and environmental stressors induce hemocyanin expression, but the involvement of hemocyanin in ER stress response is unknown. We demonstrate that in response to pathogenic bacteria (Vibrio parahaemolyticus and Streptococcus iniae), hemocyanin, ER stress proteins (Bip, Xbp1s, and Chop), and sterol regulatory element binding protein (SREBP) are induced to alter fatty acid levels in Penaeus vannamei. Interestingly, hemocyanin interacts with ER stress proteins to modulate SREBP expression, while ER stress inhibition with 4-Phenylbutyric acid or hemocyanin knockdown attenuates the expression of ER stress proteins, SREBP, and fatty acid levels. Contrarily, hemocyanin knockdown followed by tunicamycin treatment (ER stress activator) increased their expression. Thus, hemocyanin mediates ER stress during pathogen challenge, which consequently modulates SREBP to regulate the expression of downstream lipogenic genes and fatty acid levels. Our findings reveal a novel mechanism employed by penaeid shrimp to counteract pathogen-induced ER stress.

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“…Shrimp hepatopancreas lysates were prepared as previously described [ 65 ] and used for enzyme-linked immunosorbent assay (ELISA) to validate the GS-MS data. Briefly, hepatopancreas samples were gently minced in 1 mL of 0.01 M PBS (pH 7.2) before being strained through a 150-mm steel mesh and centrifuged at 200 g (4°C for 10 min) to collect the cells.…”

Section: Methodsmentioning

confidence: 99%

Taurine metabolism is modulated in Vibrio-infected Penaeus vannamei to shape shrimp antibacterial response and survival

et al. 2022

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Background Numerous microorganisms are found in aquaculture ponds, including several pathogenic bacteria. Infection of cultured animals by these pathogens results in diseases and metabolic dysregulation. However, changes in the metabolic profiles that occur at different infection stages in the same ponds and how these metabolic changes can be modulated by exogenous metabolites in Penaeus vannamei remain unknown. Results Here, we collected gastrointestinal tract (GIT) samples from healthy, diseased, and moribund P. vannamei in the same aquaculture pond for histological, metabolic, and transcriptome profiling. We found that diseased and moribund shrimp with empty GITs and atrophied hepatopancreas were mainly infected with Vibrio parahaemolyticus and Vibrio harveyi. Although significant dysregulation of crucial metabolites and their enzymes were observed in diseased and moribund shrimps, diseased shrimp expressed high levels of taurine and taurine metabolism-related enzymes, while moribund shrimp expressed high levels of hypoxanthine and related metabolism enzymes. Moreover, a strong negative correlation was observed between taurine levels and the relative abundance of V. parahaemolyticus and V. harveyi. Besides, exogenous taurine enhanced shrimp survival against V. parahaemolyticus challenge by increasing the expression of key taurine metabolism enzymes, mainly, cysteine dioxygenase (CDO) and cysteine sulfinic acid decarboxylase (CSD). Conclusions Our study revealed that taurine metabolism could be modulated by exogenous supplementation to improve crustacean immune response against pathogenic microbes.

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The ARM repeat domain of hemocyanin interacts with MKK4 to modulate antimicrobial peptides expression

Cited by 15 publications

References 72 publications

Differential responses of hepatopancreas transcriptome between fast and slow growth in giant freshwater prawns (Macrobrachium rosenbergii) fed a plant-based diet

Differential responses of hepatopancreas transcriptome between fast and slow growth in giant freshwater prawns (Macrobrachium rosenbergii) fed a plant-based diet

Modulation of SREBP Expression and Fatty Acid Levels by Bacteria-Induced ER Stress Is Mediated by Hemocyanin in Penaeid Shrimp

Taurine metabolism is modulated in Vibrio-infected Penaeus vannamei to shape shrimp antibacterial response and survival

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