The ARp1 Aspergillus Replicating Plasmid

Clutterbuck, John; Robertson, Scott

doi:10.1007/978-1-4899-0981-7_13

Cited by 5 publications

(1 citation statement)

References 4 publications

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“…AMA1-bearing plasmids replicating in mycelium are structurally stable and do not integrate into the chromosome . The AMA1 element is fully or partially functional in several species of fungi (Moreno et al, 1994;Clutterbuck et al, 1994;Bruckner et al, 1992;Kelly et al, 1994;.…”

Section: Introductionmentioning

confidence: 99%

The plasmid replicator AMA1 in Aspergillus nidulans is an inverted duplication of a low‐copy‐number dispersed genomic repeat

Aleksenko

Clutterbuck

1996

Molecular Microbiology

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The AMA1 sequence was isolated from a genomic library of Aspergillus nidulans on the basis of its ability to enhance transformation frequency and generate phenotypically unstable transformants in this fungus. These properties were previously shown to be the result of extrachromosomal replication of AMA1-bearing plasmids. Here we demonstrate that AMA1 is an inverted duplication of a sequence which has other isolated genomic copies. These sequences (mobile Aspergillus transformation enhancers, or MATEs) share a high degree of sequence similarity and exhibit some features characteristic of mobile elements, including a potential Met-tRNA priming site, similar to that found in retrotransposons of the Ty-copia group. The nucleotide sequence does not encode any extended polypeptides but contains ARS-consensus matches and a multiply repeated 'Spe' motif, which may be described as a symmetrically duplicated topoisomerase I recognition site. This motif was shown to be a target for illegitimate recombination events. The mobility of members of the MATE family is inferred from the observation that their chromosomal locations are highly variable between wild Aspergillus isolates. The inverted duplication AMA1 is present in laboratory strains derived from the Glasgow isolate but not in other wild isolates tested. This indicates that the inverted duplication AMA1 is of recent evolutionary origin and probably does not exert any conserved function in the chromosome. We discuss possible connections between structural features of AMA1 and its ability to promote extrachromosomal plasmid replication.

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Section: Introductionmentioning

confidence: 99%

The plasmid replicator AMA1 in Aspergillus nidulans is an inverted duplication of a low‐copy‐number dispersed genomic repeat

Aleksenko

Clutterbuck

1996

Molecular Microbiology

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Integrative and replicative transformation of Penicillium canescens with a heterologous nitrate-reductase gene

et al. 1995

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A wild isolate of Penicillium canescens was subjected to mutagenesis, and 150 chlorate-resistant mutants were isolated and classified in respect of their ability to utilize various nitrogen sources. Strains supposedly deficient in nitrate reductase have been transformed with the nitrate-reductase gene from Aspergillus niger. Transformation probably occurred by non-homologous integration of the transforming vector into the chromosome. Co-transformation with the AMA 1 replicating element from A. nidulans enhanced transformation frequency up to 2000-fold, and was shown to result in autonomous maintenance of replicating concatenates, one of which was re-isolated by transformation of E. coli.

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Recombinational stability of replicating plasmids in Aspergillus nidulans during transformation, vegetative growth and sexual reproduction

Aleksenko

Clutterbuck

1995

Curr Genet

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Plasmids containing the AMA1 replicon are capable of autonomous maintenance in Aspergillus nidulans. It has been reported previously that these plasmids can form concatenates by recombination in a transformed mycelium, and up to 10% of molecules are involved in such events. The present study demonstrates that plasmid recombination, although frequent during transformation, rarely occurs during vegetative growth. As a result, the structure and phenotypic stability of AMA1 plasmids generally remains unaltered for many asexual (conidial) generations. It is also evident that plasmid replication does not require specific recombination events in the AMA1 palindrome. However, during sexual reproduction, autonomous plasmids exhibit increased recombination, which results in both plasmid concatenation and integration into the chromosome.

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The ARp1 Aspergillus Replicating Plasmid

Cited by 5 publications

References 4 publications

The plasmid replicator AMA1 in Aspergillus nidulans is an inverted duplication of a low‐copy‐number dispersed genomic repeat

The plasmid replicator AMA1 in Aspergillus nidulans is an inverted duplication of a low‐copy‐number dispersed genomic repeat

Integrative and replicative transformation of Penicillium canescens with a heterologous nitrate-reductase gene

Recombinational stability of replicating plasmids in Aspergillus nidulans during transformation, vegetative growth and sexual reproduction

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