The Array Biosensor: Portable, Automated Systems

Ligler, Frances S.; Sapsford, Kim E.; Golden, Joel P.; Shriver‐Lake, Lisa C.; Taitt, Chris R.; Dyer, Maureen A.; Barone, Salvatore; Myatt, C. J.

doi:10.2116/analsci.23.5

Cited by 132 publications

(92 citation statements)

References 41 publications

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“…This generates an optical signal, the strength of which is proportional to specific analyte binding. To convert these assays from a laboratory-based 96-well plate format to a smaller, more portable biosensor system, optical fibers have been employed for the detection of whole bacterial cells (35,36). Fiber optic biosensors (FOB) typically comprise a source of light which passes through optical fibers containing immobilized bioreceptors to a photon detector.…”

Section: Optical Biosensorsmentioning

confidence: 99%

Biosensors for Whole-Cell Bacterial Detection

et al. 2014

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SUMMARY Bacterial pathogens are important targets for detection and identification in medicine, food safety, public health, and security. Bacterial infection is a common cause of morbidity and mortality worldwide. In spite of the availability of antibiotics, these infections are often misdiagnosed or there is an unacceptable delay in diagnosis. Current methods of bacterial detection rely upon laboratory-based techniques such as cell culture, microscopic analysis, and biochemical assays. These procedures are time-consuming and costly and require specialist equipment and trained users. Portable stand-alone biosensors can facilitate rapid detection and diagnosis at the point of care. Biosensors will be particularly useful where a clear diagnosis informs treatment, in critical illness (e.g., meningitis) or to prevent further disease spread (e.g., in case of food-borne pathogens or sexually transmitted diseases). Detection of bacteria is also becoming increasingly important in antibioterrorism measures (e.g., anthrax detection). In this review, we discuss recent progress in the use of biosensors for the detection of whole bacterial cells for sensitive and earlier identification of bacteria without the need for sample processing. There is a particular focus on electrochemical biosensors, especially impedance-based systems, as these present key advantages in terms of ease of miniaturization, lack of reagents, sensitivity, and low cost.

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Section: Optical Biosensorsmentioning

confidence: 99%

Biosensors for Whole-Cell Bacterial Detection

et al. 2014

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“…Array biosensor (Naval Research Laboratory) 24 uses a sandwich assay for detection of biological agents which are detected by a CCD camera. This device can simultaneously detect and identify different targets on the same slide, if multiple 'capture' or 'tracer' antibodies for different BW agent are used.…”

Section: Biosensor/immunosensormentioning

confidence: 99%

Systems for Detection and Identification of Biological Aerosols (Review Paper)

Švábenská¹

2012

DSJ

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Easy and inexpensive manufacturing of biological weapons, their complicated detection, expensive protection, difficult treating of affected individuals, selective impact only for people, animals or plants, are all factors making an effective defense against biological warfare agents very difficult. The aim of this study is an introduction to the systems for the detection and identification of biological aerosols containing dangerous bioagents. The basic techniques used for detection and identification of bioagents are described, including physical, molecular, immunochemical, and other ligand assays. Measuring systems and equipment for the individual techniques are summarised.

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“…A few commercial biosensors have been developed specifically for food analysis. One such example is the surface plasmon resonance (SPR)Ͳ based Biacore Q (Ferguson et al, 2005) with others being various adaptations of the NRL array biosensor (Ligler et al, 2007). These biosensors have many attributes highly sought after for routine food contaminant analysis such as easeͲofͲuse, fast assay times, sensitivity, good repeatability and reproducibility (GE Healthcare, 2011) with the prototype NRL array biosensor showing multiplexing capabilities (Taitt et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Development of a rapid low cost fluorescent biosensor for the detection of food contaminants

McGrath¹,

Andersson

Campbell³

et al. 2013

Biosensors and Bioelectronics

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A prototype fluorescent based biosensor has been developed for the antibody based detection of food related contaminants. Its performance was characterised and showed a typical antibody binding signal of 200Ͳ2000 mV, a short term noise of 9.1 mV, and baseline slope of Ͳ0.016 mV/s over 4 hours. Bulk signal detection repeatability (n=23) and reproducibility (n=3) were less than 2.4 %CV. The biosensor detection unit was evaluated using two food related model systems proving its ability to monitor both binding using commercial products and inhibition through the development of an assay. This assay development potential was evaluated by observing the biosensor's performance whilst appraising several labelled antibody and glass slide configurations. The molecular interaction between biotin and an antiͲbiotin antibody was shown to be inhibited by 41% due to the presence of biotin in a sample. A food toxin (domoic acid) calibration curve was produced, with %CVs ranging from 2.7 to 7.8%, and a midpoint of approximately 17 ng/ml with further optimisation possible. The ultimate aim of this study was to demonstrate the working principles of this innovative biosensor as a potential portable tool with the opportunity of interchangeable assays. The biosensor design is applicable for the requirements of routine food contaminant analysis, with respect to performance, functionality and cost.

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The Array Biosensor: Portable, Automated Systems

Cited by 132 publications

References 41 publications

Biosensors for Whole-Cell Bacterial Detection

Biosensors for Whole-Cell Bacterial Detection

Systems for Detection and Identification of Biological Aerosols (Review Paper)

Development of a rapid low cost fluorescent biosensor for the detection of food contaminants

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