The arsenal of virulence factors deployed by<i>Listeria monocytogenes</i>to promote its cell infection cycle

Camejo, Ana; Carvalho, Frédéric A.; Reis, Olga; Leitão, Elsa; Sousa, Sandra; Cabanes, Didier

doi:10.4161/viru.2.5.17703

Cited by 196 publications

(227 citation statements)

References 219 publications

(252 reference statements)

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“…A comprehensive set of 115 genes identified as putative or confirmed virulence factors were used from the two studies (Camejo et al, 2011;Maury et al, 2016). The gene sequences were extracted from L. monocytogenes EGD-e (accession NC_003210.1) apart from the LIPI3 clusters of gene is extracted from L. monocytogenes F2365.…”

Section: Published Virulence Factorsmentioning

confidence: 99%

Closing gaps for performing a risk assessment on Listeria monocytogenes in ready‐to‐eat (RTE) foods: activity 3, the comparison of isolates from different compartments along the food chain, and from humans using whole genome sequencing (WGS) analysis

Nielsen

Björkman

Kiil

et al. 2017

EFSA Supporting Publications

111

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This report presents the results of the project "Closing gaps for performing a risk assessment on Listeria monocytogenes in ready-to-eat (RTE) foods: activity 3, the comparison of isolates from different compartments along the food chain, and from humans using whole genome sequencing (WGS) analysis". The main objective was to compare L. monocytogenes isolates collected in the EU from ready-to-eat (RTE) foods, compartments along the food chain and from human cases by the use of WGS. A total of 1,143 L. monocytogenes isolates were selected for the study, including 333 human clinical isolates and 810 isolates from the food chain. The isolates were whole genome sequenced. The phylogeny showed a clear delineation between L. monocytogenes lineages and between clonal complexes within lineages. A range of typing methods were applied to the sequence data, providing the framework to answer questions on genetic diversity and epidemiological relationships. Retrospective analysis of nine outbreaks showed that WGS is a powerful tool in national and international outbreak investigations as WGS can accurately rule isolates in or out of outbreaks. Source attribution models showed bovine reservoir to be the main source of human disease although other sources also contributed and generally confidence intervals were high. Numerous consistent genetic linkages between a priori unlinked strains were identified, some of which involved isolates from multiple countries. The presence of putative markers conferring the potential to survive/multiply in the food chain and/or cause disease in humans was explored by detecting the presence of putative virulence genes, AMR genes and factors conferring the ability to persist in the food processing chain. This study has demonstrated one of the major benefits of WGS, which is the ability to address a wide range of questions including those on virulence, antimicrobial resistance, source attribution, surveillance and outbreak detection and investigation, in a single experiment.

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Section: Published Virulence Factorsmentioning

confidence: 99%

Closing gaps for performing a risk assessment on Listeria monocytogenes in ready‐to‐eat (RTE) foods: activity 3, the comparison of isolates from different compartments along the food chain, and from humans using whole genome sequencing (WGS) analysis

Nielsen

Björkman

Kiil

et al. 2017

EFSA Supporting Publications

111

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“…They include the secreted listeriolysin O (LLO) and two phospholipases involved in the disruption of phagosomal membranes and bacterial escape to the cytoplasm Mengaud et al 1987;Váz-quez-Boland et al 1992), the surface protein ActA that mediates the polymerization of cytoplasmic actin (Domann et al 1992;Kocks et al 1992) and favors cell-to-cell spread (Tilney and Portnoy 1989) as well as PrfA, the transcriptional activatorof bacterial virulence genes (Leimeister-Wächter et al 1990;Mengaud et al 1991). Comparative genomic approaches on the pathogenic L. monocytogenes and the nonpathogenic Listeria innocua has led in recent years to the identification of many additional bacterial factors required for infection (Camejo et al 2011;Cossart 2011). In this article, we will summarize our current understanding of bacterial effectors and cellular signaling cascades that are subverted by L. monocytogenes in order to invade mammalian epithelial cells.…”

mentioning

confidence: 99%

Entry of Listeria monocytogenes in Mammalian Epithelial Cells: An Updated View

Pizarro‐Cerdá

Kühbacher

Cossart

2012

Cold Spring Harbor Perspectives in Medicine

226

203

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Listeria monocytogenes is a bacterial pathogen that promotes its internalization into host epithelial cells. Interaction between the bacterial surface molecules InlA and InlB and their cellular receptors E-cadherin and Met, respectively, triggers the recruitment of endocytic effectors, the subversion of the phosphoinositide metabolism, and the remodeling of the actin cytoskeleton that lead to bacterial engulfment. Additional bacterial surface and secreted virulence factors also contribute to entry, albeit to a lesser extent. Here we review the increasing number of signaling effectors that are reported as being subverted by L. monocytogenes during invasion of cultured cell lines. We also update the current knowledge of the early steps of in vivo cellular infection, which, as shown recently, challenges previous concepts generated from in vitro data.

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“…1 Lm employs an arsenal of virulence factors to invade, survive, and multiply in both phagocytic and non-phagocytic cells, 2 hijacking host signaling pathways to establish and sustain infection. 3 Several bacterial pathogens were shown to modulate the host cell cycle to support infection.…”

Section: Introductionmentioning

confidence: 99%

Listeria monocytogenesinduces host DNA damage and delays the host cell cycle to promote infection

et al. 2014

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The arsenal of virulence factors deployed byListeria monocytogenesto promote its cell infection cycle

Abstract: Cabanes (2011) The arsenal of virulence factors deployed by Listeria monocytogenes to promote its cell infection cycle, Virulence, 2:5, 379-394,

Cited by 196 publications

References 219 publications

Closing gaps for performing a risk assessment on Listeria monocytogenes in ready‐to‐eat (RTE) foods: activity 3, the comparison of isolates from different compartments along the food chain, and from humans using whole genome sequencing (WGS) analysis

Closing gaps for performing a risk assessment on Listeria monocytogenes in ready‐to‐eat (RTE) foods: activity 3, the comparison of isolates from different compartments along the food chain, and from humans using whole genome sequencing (WGS) analysis

Entry of Listeria monocytogenes in Mammalian Epithelial Cells: An Updated View

Listeria monocytogenesinduces host DNA damage and delays the host cell cycle to promote infection

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