The aryl hydrocarbon receptor in β-cells mediates the effects of TCDD on glucose homeostasis in mice

Characterizing the effects of Dechlorane Plus on β-cells: a comparative study across models and species

“…All experiments involving primary mouse islets were approved by the Carleton University Animal Care Committee (AUP #113152 and #114477) and carried out in accordance with the Canadian Council on Animal Care guidelines. Islets were isolated by pancreatic duct injection with collagenase (1,000 units/ml; Sigma Aldrich, #C7657) dissolved in Hanks’ balanced salt solution (HBSS: 137 mM NaCl, 5.4 mM KCl, 4.2 mM NaH 2 PO 4 , 4.1 mM KH 2 PO 4 , 10 mM HEPES, 1 mM MgCl 2 , 5 mM dextrose, pH 7.2), as previously described 28 . Briefly, pancreas tissues were incubated at 37°C for 10.5–11 minutes, agitated, and the collagenase reaction quenched with ice-cold HBSS with 1 mM CaCl 2 .…”