The Aspergillus niger niaD gene encoding nitrate reductase: upstream nucleotide and amino acid sequence comparisons

Unkles, Shiela E.; Campbell, Edward I.; Punt, Peter J.; Hawker, Kim L.; Contreras, Rodrigo A.; Hawkins, Alastair R.; Hondel, Cees A. M. J. J. van den; Kinghorn, James R.

doi:10.1016/0378-1119(92)90682-f

Cited by 58 publications

(26 citation statements)

References 22 publications

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“…Although homologs of A. nidulans nitrate assimilation and metabolism genes have been identified in several fungi (Diolez et al, 1993;Unkles et al, 1992) and the oomycete Phytophthora infestans (Pieterse et al, 1995), niiA and niaD linkage is not always conserved. Still, the fact that niiA and niaD clusters have been reported in the yeast Hansenula polymorpha (Brito et al, 1996) is evidence that a nutrient utilization pathway cluster can be widespread in the fungal kingdom.…”

Section: Id Nitrate Assimilationmentioning

confidence: 98%

Metabolic Pathway Gene Clusters in Filamentous Fungi

Keller

Höhn

1997

Fungal Genetics and Biology

556

370

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Section: Id Nitrate Assimilationmentioning

confidence: 98%

Metabolic Pathway Gene Clusters in Filamentous Fungi

Keller

Höhn

1997

Fungal Genetics and Biology

556

370

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“…The clustering of genes involved in nitrate assimilation occurs in some organisms like A. nidulans [63 A. niger [7] U. maydis [8] and the alga C. reinhardtii [24]. However, in N. crassa as well as in plants the genes involved in nitrate assimilation are unlinked [25].…”

Section: £ ! Molecular Cloning Of the Nitrate Reductase Gene (Ynri)mentioning

confidence: 99%

“…Two degenerated oligonucleotides were designed on the basis of protein sequence similarity of NRs from N. crassa, A. nidulans and A. niger [5][6][7]. The degenerated oligonucleotide mixtures were:…”

Section: Synthesis Of a Homologous Ynri Probe By Pcrmentioning

confidence: 99%

“…Several genes are responsible for the production of an active NR [4]: (a) the gene coding the NR apoenzyme; (b) the genes involved in the synthesis of the MoCo; (c) regulatory genes involved in the expression of the NR gene. Assimilatory NR genes have been isolated from filamentous fungi [5][6][7][8], plants [9][10][11] and algae [12] but not from yeasts. Due to the sophistication level of molecular genetic techniques in yeaat, the cloning of the gene YNRI encoding NR in this organism offers great possibilities to study further the nitrate assimilation pathway and the regulation of this enzyme.…”

Section: Introductionmentioning

confidence: 99%

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Cloning and disruption of the YNR1 gene encoding the nitrate reductase apoenzyme of the yeast Hansenula polymorpha

Ávila¹,

Pérez²,

Brito³

et al. 1995

FEBS Letters

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“…Six phenotypic classes of chlorate resistant mutants (nrtA, niiA, niaD, cnx, nirA and areA) were characterized and designated as in Aspergillus nidulans (9,13,20). Genetic studies revealed that niaD and niiA genes are the structural gene for nitrate and nitrite reductase enzymes respectively, (11,31). The transcription of both genes depends on two positively acting transcription factors, one is encoded by the nirA gene, (mediates nitrate and nitrite induction) and required for efficient transcription of niaD, niiA, and nrtA (6,27).…”

Section: Introductionmentioning

confidence: 99%

Isolation and growth characterization of chlorate and/or bromate resistant mutants generated by spontaneous and induced foreword mutations at several gene loci in Aspergillus niger

Kanan¹,

Al-Najjar²

2010

Braz. J. Microbiol.

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We aimed her mainly to evaluate the contribution of newly employed bromate selection system, in obtaining new Aspergillus niger nitrate/nitrite assimilation defective mutants, through Ultraviolet treatment (UV), 1, 2, 7, 8-Diepoxyoctane (DEO), phenols mixture (Phx)) and spontaneous treatments. The newly employed bromate selection system was able to specify only two putative novel mutant types designated brn (bromate resistant but chlorate sensitive (RS) strain, which may specify nitrite specific transporter) and cbrn mutants (bromate resistant and chlorate resistant strain, which may specify nitrate/nitrite bispecific system). The most relevant and innovative findings of this research work involve the isolation of the RR (cbrn) mutants (a new type of nitrate assimilation defective mutants), that could be useful for studying the bispecific nitrate /nitrite transporter system. The majority of obtained bromate resistant mutants (93.3% of the total mutants obtained by all treatments) were of the brn type, whereas the remaining percentage (6.76%) was given to cbrn strains. The highest percentages of brn mutant strains (48% and 58.6% of the total RS strains) were obtained with UA after spontaneous and Phx treatment, whereas Trp has generated 29% and 42% of RS strains after UV and DEO treatments, respectively. The obtained ratios of cbrn mutants were higher (i.e. in the range of 8.4%-11.64% of the total bromate mutants) with chemical treatments, especially when U.A or Pro was serving as sole N-sources at 25ºC rather than 37ºC. A 69% mutants` yield of Aspergillus niger mutant strains representing nine gene loci (niaD, cnx-6 loci, nrt and nirA) were selected on the bases of chlorate (600 mM) toxicity. All chlorate resistant mutants were completely sensitive to bromate (250 mM). The niaD mutants showed the highest percentage (73.97%) of chlorate resistant mutants obtained with all tested treatments. The UV treatment has generated the highest ratio (86.9%) of niaD mutants, whereas, the least (61%) was obtained with Phx treatment. The highest percentage of cnx mutants (32%) was obtained with Phx treatment. The DEO treatment as compared to other tested treatments was the best to use for obtaining the highest ratios of either nrt (13.8%) mutants or nirA (1.9%) mutants.

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The Aspergillus niger niaD gene encoding nitrate reductase: upstream nucleotide and amino acid sequence comparisons

Cited by 58 publications

References 22 publications

Metabolic Pathway Gene Clusters in Filamentous Fungi

Metabolic Pathway Gene Clusters in Filamentous Fungi

Cloning and disruption of the YNR1 gene encoding the nitrate reductase apoenzyme of the yeast Hansenula polymorpha

Isolation and growth characterization of chlorate and/or bromate resistant mutants generated by spontaneous and induced foreword mutations at several gene loci in Aspergillus niger

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