The assay of glutethimide in plasma

Disposition of glutethimide in man

“…7 In brief, glutethimide was extracted from the aqueous solutions into dichloromethane or, for milk only, chloroform. The extract was washed with alkali, acid, and water before concentration by evaporation.…”

“…Glutethimide was assayed in plasma, whole blood, urine, milk, plasma dialysate, and saturated aqueous solutions by solvent extraction and gas chromatography; the method has been described in full elsewhere. 7 In brief, glutethimide was extracted from the aqueous solutions into dichloromethane or, for milk only, chloroform. The extract was washed with alkali, acid, and water before concentration by evaporation.…”

“…The late absorption of a small proportion of a dose was, however, recognized earlier as a complicated factor in the use of plasma level data in measuring rates of metabolism and excretion. 7 The wide variety of times to reach the peak level suggested the occurrence of a corresponding wide range in absorption rates. Fig.…”

“…However, it became possible only recently to measure concentrations of the unchanged drug in plasma after single doses; a new gas chromatographic method was devised, and first investigations 7 of plasma levels after test doses have been reported. The present paper is concerned with: (1) plasma levels over the first 24 hours after dosage and the effect of dose on plasma levels during the first 2lh hours; (2) protein binding, red cell localization, and placental transfer; ( 3) excretion in urine and breast milk; and (4) pKa values.…”

Drug Abuse Screening Programs: Detection Procedures, Development Costs, Street-Sample Analysis, and Field Tests

“…Gas Chrom Q coated with 2 % XE-60, 3 % SE-30, or 5z DC-200 was used as the packing material. The determination of glutethimide6 in biological fluids by GLC also was reported (98,99). Recently, Baselt and Casarett (100) reported GLC methods for the routine identification of barbituratesand amphetamines; these methods are adaptations of techniques reported by Lebish e f a/.…”