The assembly of integrated rat intestinal‐hepatocyte cultures

Kothari, Anjaney; Rajagopalan, Padmavathy

doi:10.1002/btm2.10146

Cited by 5 publications

(2 citation statements)

References 31 publications

(100 reference statements)

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“…95 Other useful systems have been developed to allow coculture of intestinal explants with other cell types, such as hepatocytes, or to introduce luminal flow. 96,97 Although the complexity of intestinal explants makes them the most physiological of in vitro models, the inclusion of all of the cell types can make interpretation of experimental results challenging. If a study would benefit from a focus on the epithelium or longer culture time, it can be advantageous to use cells instead of explants.…”

Section: Explantsmentioning

confidence: 99%

In VitroModels of the Small Intestine: Engineering Challenges and Engineering Solutions

Hewes

Wilson

Estes

et al. 2020

Tissue Engineering Part B: Reviews

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Pathologies affecting the small intestine contribute significantly to the disease burden of both the developing and the developed world, which has motivated investigation into the disease mechanisms through in vitro models. Although existing in vitro models recapitulate selected features of the intestine, various important aspects have often been isolated or omitted due to the anatomical and physiological complexity. The small intestine's intricate microanatomy, heterogeneous cell populations, steep oxygen gradients, microbiota, and intestinal wall contractions are often not included in in vitro experimental models of the small intestine, despite their importance in both intestinal biology and pathology. Known and unknown interdependencies between various physiological aspects necessitate more complex in vitro models. Microfluidic technology has made it possible to mimic the dynamic mechanical environment, signaling gradients, and other important aspects of small intestinal biology. This review presents an overview of the complexity of small intestinal anatomy and bioengineered models that recapitulate some of these physiological aspects. Keywords: gut-on-a-chip, microfluidic models of intestine, small intestine model, tissue engineered intestine Impact Statement There is a need for improved cell culture models of the small intestine. To develop such models, it is necessary to give engineers a better understanding of intestinal biology, and conversely, to give biologists an idea of what is possible with microfluidic devices and biomaterial platforms. This work provides a detailed overview of aspects of intestinal biology that are important for tissue engineers to understand when designing models of the small intestine and provides a summary of current approaches. This review highlights both unique and common features of various strategies for modeling the intestinal cell environment, not only using microfluidic chip-based systems but also several elegant designs that use a materials-based static culture approach.

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Section: Explantsmentioning

confidence: 99%

In VitroModels of the Small Intestine: Engineering Challenges and Engineering Solutions

Hewes

Wilson

Estes

et al. 2020

Tissue Engineering Part B: Reviews

View full text Add to dashboard Cite

show abstract

“…The intestinal segment of the sacrificed rat was removed and cleaned using Tyrode’s liquid to eradicate all the excretory products that were available in the duodenum. The intestinal duodenum part with a length of 9.6 ± 0.6 cm and diameter of 2.56 ± 0.31 mm was filled with the formulation and solution of the MG separately comprising 75 mg of the MG in 10 ml of SLNs preparation and SOL separately ( Kothari and Rajagopalan, 2020 ). This filled segment was then placed in 100 ml of the PBS (pH 7.4) that was kept at 37 ± 0.5 °C and shaken at 100 rpm on a magnetic stirrer.…”

Section: Methodsmentioning

confidence: 99%