The assembly of wheat-associated fungal community differs across growth stages

Zheng, Yuyin; Li, Xiangzhen; Cao, Huili; Lei, Li; Zhang, Xue; Han, Dejun; Wang, Junming; Yao, Minjie

doi:10.1007/s00253-021-11550-1

Cited by 9 publications

(4 citation statements)

References 46 publications

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“…Considering all samples collectively, ASVs were categorized into 'abundant taxa' if their average relative abundances were greater than 0.1%, 'rare taxa' if under 0.01%, and 'moderate taxa' between 0.01-0.1% across all samples (Jiao and Lu 2020; Zhao et al 2023). ASVs with a consistent presence in 80% of the samples among all varieties were de ned as the core taxa and the rest were non-core taxa according to the presence/absence-based approach (Neu et al 2021;Zheng et al 2021b). Fungal community composition at phylum and genus levels for each ASV category was visualized as alluvial stacked bar charts using 'ggplot2' package.…”

Section: Sequence Data Processing and Asv Classi Cationmentioning

confidence: 99%

“…The PCR ampli cation protocol consisted of an initial denaturation step at 94°C for 5 minutes, followed by 35 cycles of ampli cation (94°C for 30 s, 56°C for 30 s, and 68°C for 30 s), and a nal extension at 72°C for 10 minutes. The puri ed PCR products from all samples were combined in equal amounts and subjected to sequencing using an Illumina Hiseq sequencer (Illumina, San Diego, CA, USA) with 2 × 250 bp paired-end reads (Zheng et al 2021b).…”

Section: Sample Preparation Dna Extraction Its Gene Ampli Cation and ...mentioning

confidence: 99%

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Linking root-associated fungal communities, wheat evolutionary patterns, and productivity: overlooked roles of rare taxa

Kang,

Zheng,

Feng

et al. 2023

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Background and Aims The intricate relationships between root-associated fungal communities and wheat plants can potentially impact plant health, crop yield, and quality. However, the interplay among various wheat varieties, fungal communities, and wheat productivity remains understudied. Methods In this study, 95 wheat varieties in China were selected. Plant and soil samples were collected during two wheat growth stages (regreening and heading). DNA extraction and genotyping were performed, and fungal ASVs were categorized based on abundance. Fungal community diversity, assembly mechanisms, and relationships were examined. Co-occurrence networks were used to identify keystone taxa. Relationships between wheat genetics, quality, yield, and fungal communities were assessed. Broad-sense heritability (H2) of root-associated ASVs was calculated. Results Abundant and core taxa significantly influenced fungal community structure, with stochastic processes mainly driving the assembly. Regreening stage's rhizosphere harbored keystone taxa, though a weak but meaningful connection existed between wheat genetic distance and rare taxa at the heading stage. Wheat quality and yield were significantly (P < 0.05) influenced by rare rhizosphere taxa during regreening stage, while heading stage yield correlated positively with keystone taxa and Glomeromycota. Rhizosphere abundant subcommunity was more specialized in enriching taxa with low and moderate heritability, while rare subcommunity was better at recruiting high-heritability (H2 > 0.4) taxa. Conclusions Abundant and core taxa played a substantial role in shaping fungal communities during wheat growth, but less abundant rhizosphere species were also vital, as they attained keystone status, showed genetic ties to the host, and were related to wheat quality and yield with higher heritability.

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Section: Sequence Data Processing and Asv Classi Cationmentioning

confidence: 99%

Section: Sample Preparation Dna Extraction Its Gene Ampli Cation and ...mentioning

confidence: 99%

Linking root-associated fungal communities, wheat evolutionary patterns, and productivity: overlooked roles of rare taxa

Kang,

Zheng,

Feng

et al. 2023

Preprint

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“…DSI and DI were used to evaluate the effect extent of each enrichment process. The higher DSI value represents greater differences between each compartment niche and bulk soil, and the higher DI value represents a greater depleted effect (Xiong et al, 2021;Zheng et al, 2021a).…”

Section: Dsi and DI Indicesmentioning

confidence: 99%

The effects of P on wheat root-associated prokaryotic communities are dependent on soil P content, growth stage and variety

Feng¹,

Li²,

Li³

et al. 2022

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Background Wheat root-associated microbes play an indispensable role in helping plants obtain phosphorus (P) from soil. However, it is not clear how the effects of soil P on microorganisms vary with growth stage and variety. Therefore, revealing the interactive effects of soil P content, growth stage and variety on wheat root-associated microorganisms, which is important for further improving the P absorption strategy of plants. Methods Three wheat varieties were selected for pot experiment under three levels of soil P content, and sampling at seedling, flowering and mature stage, respectively. Using 16S rRNA gene profiling, we investigated the relative contribution of compartment niche, growth stage, variety and soil P content and their interaction effects on wheat root-associated prokaryotic communities. Results Wheat root-associated prokaryotic community assembly was mainly shaped by compartment niches. For each compartment niche, growth stage had higher contribution to the variations of prokaryotic communities than soil P content and wheat variety. The effects of P content on the prokaryotic communities varied by growth stage and variety. Meanwhile, the effects of wheat variety were also influenced by growth stage and soil P content. It is worth mentioning that low P content exacerbated the variation of prokaryotic communities among varieties. Conclusions This study revealed the interaction of host selection and soil P content on root-associated prokaryotic communities. The effects of soil P on wheat root-associated prokaryotic communities are dependent on P content, growth stage and variety. Our study provides a fundamental understanding on the relationship between soil P content and microorganisms.

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“…Changes in plant growth may further lead to variations in the plant-associated fungal community structure (Seghers et al, 2004 ; Larkin et al, 2012 ; Suryanarayanan and Shaanker, 2021 ). Furthermore, over different growth stages of the host plant, the plant endophytic fungal community is likely subject to dynamic changes over time (Singha et al, 2021 ; Sosso et al, 2021 ; Zheng et al, 2021 ). Several studies have provided some insights into how FEFs respond to high N supply.…”

Section: Introductionmentioning

confidence: 99%

Can nitrogen supersede host identity in shaping the community composition of foliar endophytic fungi in an alpine meadow ecosystem?

et al. 2022

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The availability of limiting nutrients plays a crucial role in shaping communities of endophytes. Moreover, whether fungal endophytes are host-specific remains controversial. We hypothesized that in a harsh and nitrogen (N)-deficient area, diversity and community composition of foliar endophytic fungi (FEFs) varied substantially among plots with experimentally elevated levels of macronutrients, and thus, N availability, instead of host species identity, would have a greater influence in structuring fungal communities at different scales. We also expected an important subset of taxa shared among numerous host species and N gradients to form a community-wide core microbiome. We measured the leaf functional traits and community structures of FEFs of three commonly seen species in an alpine meadow nested with a long-term N fertilization experiment. We found that host plant identity was a powerful factor driving the endophytic fungal community in leaves, even in habitats where productivity was strongly limited by nitrogen (p < 0.001). We also found that within the same host, nitrogen was an important driving force for the composition of the endophytic fungi community (p < 0.05). In addition, the leaf carbon content was the most important functional trait that limited the diversity of endophytic fungi (p < 0.001). Finally, we documented a distinct core microbiome shared among our three focal species and N gradients.

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The assembly of wheat-associated fungal community differs across growth stages

Cited by 9 publications

References 46 publications

Linking root-associated fungal communities, wheat evolutionary patterns, and productivity: overlooked roles of rare taxa

Linking root-associated fungal communities, wheat evolutionary patterns, and productivity: overlooked roles of rare taxa

The effects of P on wheat root-associated prokaryotic communities are dependent on soil P content, growth stage and variety

Can nitrogen supersede host identity in shaping the community composition of foliar endophytic fungi in an alpine meadow ecosystem?

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