The assessment and application of a bacteriocin typing scheme for<i>Clostridium perfringens</i>

Watson, G. N.

doi:10.1017/s0022172400061143

Cited by 21 publications

(11 citation statements)

References 17 publications

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“…An analogous method has • been recommended for a similar purpose also in other gram-positive species (Watson 1985).…”

Section: Discussionmentioning

confidence: 99%

Bacteriocin Typing of Staphylococci

Skalka¹

1992

Acta Vet. Brno

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Skalka, B.: Bacteriocin Typing of Staphylococci. Acta vet. Bmo, 61,1992: 179-187.The report describes bacteriocin typing of staphylococcal strains using the deferred method. The collection of active strains comprised 11 strains, Q of which were isolated by the author, namely 3 S. intermedius strains, 2 S. hominis strains and 1 strain each of S. epidermidis, S. simulans, S. sciuri and S. haemolyticus, and two additional strains, namely S. aureus producing bacteriocin Bac Rl and S. simulans biovar staphylolyticus. The active strains varied in spectrum and intensity of their effects on both indicator stains and examined strains. Using the active strains a total of 2 083 staphylococcal strains representing a great majority of known staphylococcal species were typed. The highest sensitivity was shown by coagulase-positive species, lower sensitivity was found in coagulase-negative, novobiocin-sensitive species and the lowest sensitivity was found in coagulase-negative, novobiocin-resistant species. The bacteriocin typing described in the report may prove useful for detailed characteristics of staphylococcal strains. Bacteriocin typing, deferred method, Staphylococcus spp., active strains, indicator strains, examined strainsOur previous reports on antibiotic products of staphylococci have described the characteristics of bacteriocins, also called staphylococcins, bacteriocin-like substances, together with information on their frequency within the genus Staphylococcus and their application in presumptive bacteriocin typing of staphylococci (Skalka 1985(Skalka , 1986a(Skalka , 1986b. Till now practical utilization of staphylococcins and staphylococcin-like staphylococcal antibiotic substances has been limited to laboratory studies in spite of some Folitary descriptions of their successful clinical use in staphylococcal infections (Lachowicz 1965, Gerber andNowak 1976) and in the therapy of a skin disease of Dermatophilus congolensis aetiology (Zaria 1991). Laboratory use of staphylococcin producers has been described by Ivanov (1970, J etten andVogels (1972), Pulverer and J eljaszewicz (l976) and Smarda and ObdrHlek (1981). The object of the present study is to present our own set of active strains and its use in bacteriocin typing of staphylococcal species. Materials and Methods Nutrient MediaBrain Heart Infusion Agar CM 375 (Oxoid) and Blood Agar Base No. 4 (Imuna) were used. Bacterial StrainsBacterial strains were divided into the following three groups: a set of active strains or producers of antibiotic substances, a set of known indicator strains and a set of strains to be examined. The identification of staphylococci and the biotyping of S. aureus were carried out on the basis of generally accepted recommendations (Devriese 1984, Kloos andSchleifer 1986).The list of active and indicator strains gives their working designation (W), species, original laboratory designation (L) and Collection code (C).

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“…An analogous method has • been recommended for a similar purpose also in other gram-positive species (Watson 1985).…”

Section: Discussionmentioning

confidence: 99%

Bacteriocin Typing of Staphylococci

Skalka¹

1992

Acta Vet. Brno

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“…Bacteriocin typing and sesotyping represent the two methods most commonly used for strain differentiation of Closhdiam pefingens (STRINGER et al, 1980;Scorn and UHONY, 1982;WATSON, 1985;LABBE, 1989). Plasmid typing appears to be suitable for the differentiation of C.pe@ngens strains (MAHONY et al, 1986(MAHONY et al, , 1987PHILLIPS JONES et al, 1989;EISGRUBER et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Strain Differentiation of Clostridium perfringens by Bacteriocin Typing, Plasmid Profiling and Ribotyping

Schalch

Eisgruber

Schau

et al. 1998

Journal of Veterinary Medicine, Series B

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Bacteriocin typing, plasmid profiling and ribotyping were used to type 34 food and patient Closhdium pe@ngensisolates from 10 food poisoning cases, respectively, outbreaks. In nine cases/outbreaks bacteriocin patterns showed identical main groups. Subgroups differed w i h all cases/outbreaks. Plasmid profiles were identical for all isolates within each of three outbreaks. In eight food poisoning cases and outbreaks, all the ribotypes of each food and stool isolate were found to be identical. All three typing methods give valuable results for the characterization of C pe&ngens beyond the species level. Bacteriocin typing represents a suitable addition to plasmid typing, particularly since the results do not show any correlation between losses of plasmids and changes in bacteriocin sensitivity patterns. Ribotyping was found to be a suitable tool to determine the genetic relationship of C. petjimgens isolates in the context of food-borne poisoning.

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“…A variety of typing methods for bacterial strains have been described in the literature. Common methods are biochemical characterization systems, which are also commercially available (e. g. Biolog, Hayward, CA, USA), A-148 (Erfurt) Type A-371 (Erfurt) Type C-408 (Erfurt) Type C-410 (Erfurt) 1 (27.9) 1 (7.1) 1 (27.9) 0 0 2 (33.0, 8.7) 0 2 (27.1, 23.9) 0 2 (10.3, 5.5) 2 (10.3, 5.5) 1 (9.3) 3 (21.2, 7.9, 1.2) bacteriocin typing (MAHONEY, 1974; SCO~T and MAHONEY, 1982; WATSON, 1985), antibiotic resistance profiles (GIACCA et al, 1987) and multilocus enzyme electrophoresis (BOERLIN and PIFFARETII, 1991). Examples for less frequently used typing procedures are polyacrylamide gel electrophoresis of bacterial proteins and highperformance liquid chromatography analysis of bacterial metabolic products or fatty acid profiles (BIRNBAUM et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

Plasmid Profiling for Strain Differentiation and Characterization of Clostridium perfringens Isolates

Eisgruber

Stolle

1996

Journal of Veterinary Medicine, Series B

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Summary Plasmid profiling was used for the characterization of 13 Clostridium perfringens collection strains as well as 85 clinical and food isolates. Methodological details and limitations of the plasmid isolation procedure are outlined and discussed. For 19 clinical isolates obtained during seven group outbreaks, a close connection between at least some strains from each individual outbreak was established for six of them; only results from one outbreak were completely inconclusive, due to missing plasmids in one of the two isolates. The presence of multiple plasmid types within seven out of 12 given food samples, from which at least two C. perfringens isolates were obtained, indicates the importance of multiple isolates for meaningful typing results in epidemiological investigations. By including results from a previous report from this laboratory, baseline data on plasmid profiles for a total of 133 isolates are provided. The results of this study revealed that 36 % of the food isolates unrelated to disease outbreaks carried no plasmids, as compared with 19–25 % among disease‐related isolates. A high prevalence (24.8 %) of a 8.9 ± 0.5 MDa plasmid was found among the 133 isolates, which contributed to one of four occurrences of identical plasmid profiles among strains that were initially considered unrelated. Two of these identical plasmid profiles were found among strains from the same culture collection, indicating the possibility of a common ancestor strain or cross‐contamination.

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The assessment and application of a bacteriocin typing scheme forClostridium perfringens

Cited by 21 publications

References 17 publications

Bacteriocin Typing of Staphylococci

Bacteriocin Typing of Staphylococci

Strain Differentiation of Clostridium perfringens by Bacteriocin Typing, Plasmid Profiling and Ribotyping

Plasmid Profiling for Strain Differentiation and Characterization of Clostridium perfringens Isolates

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