The assessment of the composition of the microbiota of the facial skin in patients with seborrheic dermatitis and metabolic syndrome

Abstract

Our study revealed associations with dysregulation of the community of commensals connected with seborrheic dermatitis, metabolic syndrome (MS) and type 2 diabetes mellitus.The aim of the study was to identify the association of the severity of SD and changes in the bacterial composition of the skin microbiota in patients with facial SD, with facial SD and metabolic syndrome and in patients with facial SD with MS and type 2 diabetes mellitus.Material and methods.The study included 90 patients with seborrheic dermatitis, divided into 3 groups . The severity of the disease was determined by SEDASI score . Metabolic syndrome was diagnosed based on the criteria of NCEP ATP III. The diagnosis of diabetes mellitus was established according to the diagnostic criteria of diabetes mellitus and other glycemic disorders . A modified Leming-Notman dense medium was used to isolate yeast fungi of Malassezia spp. The cultures were obtained according to the Gold’s method. The species identification from clinical isolates was carried out using the mass spectrometry method on the MALDI-TOF MS "Biotyper RTC" device.Results.Significant changes in the number of bacterial species were observed in patients with moderate to severe diabetes mellitus. We found that the colonization of microorganisms on the facial skin significantly increased in patients of groups 2 and 3, compared with group 1. Among the isolated staphylococci in patients of groups 2 and 3, the growth of S. аureus, S. epidermidis, and S. hominis prevailed.Conclusion.The severity of the duration and widespread lesions on the facial skin in patients with seborrheic dermatitis, metabolic syndrome and with seborrheic dermatitis, metabolic syndrome and type 2 diabetes mellitus are interrelated with dysbiosis of the microbiota. The growth of microorganisms in patients with seborrheic dermatitis and impaired carbohydrate metabolism is associated with severe facial SD and plays a significant role in the pathogenesis of the disease.