The assessment of the efficacy of STRs panels recommended by the ISAG for canine pedigrees analysis for forensic casework

Radko, Anna; Podbielska, Angelika; Wierzbowska, Izabela A.

doi:10.2478/aoas-2021-0085

Cited by 2 publications

(2 citation statements)

References 19 publications

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“…Thus, the MP value for mixed‐breed dog samples in the study by Kanthaswamy et al. (2018) was 1.25 × 10 −21 , and, in the work of Polish colleagues (Radko et al., 2022), it ranged from 1.25 × 10 −20 (for a standard panel) to 3.5 × 10 −23 (for an extended panel). High MP values are easily explained by the high allelic diversity of dinucleotide markers compared to tetranucleotide repeats.…”

Section: Resultsmentioning

confidence: 89%

A panel of tetranucleotide STR markers as an alternative approach to forensic DNA identification of wolf and dog

Hrebianchuk,

Parfionava,

Zabauskaya

et al. 2024

Animal Genetics

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Commercial panels of microsatellite (STR) loci are focused on the use of DNA of the domestic dog (Canis lupus familiaris) and are often inapplicable for genotyping the DNA of the gray wolf (Canis lupus lupus). We propose a CPlex test system, including one hexa‐ and 12 tetranucleotide autosomal STR loci, as well as two sex loci, that is equally efficient in DNA identification of biological samples of the wolf and the dog. Analysis of molecular variance between samples revealed significant differentiation values (FST = 0.0784, p < 0.001), which allows to use the panel to differentiate wolf and dog samples. Population subdivision coefficients (θ‐values) were calculated for each of the 13 STR loci of the developed test system. It was shown that the values of the genotype frequency for dogs and wolves, without and with considering the θ‐value, differ by three orders of magnitude (for dogs 8.9 × 10−16 and 2.1 × 10−14 and for wolves 1.9 × 10−15 and 4.5 × 10−14, respectively). The use of population subdivision coefficients will allow to identify the most reliable results of an expert identification study and the power of exclusion provided by the STR loci of the CPlex test system makes it possible to achieve a reliable level of evidence in forensic DNA analysis of both wolves and dogs. The test system has been validated for use in forensic identification of the dog and wolf based on biological traces found at crime scenes, as well as for individual identification and establishing biological relationship of animals of these species.

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Section: Resultsmentioning

confidence: 89%

A panel of tetranucleotide STR markers as an alternative approach to forensic DNA identification of wolf and dog

Hrebianchuk,

Parfionava,

Zabauskaya

et al. 2024

Animal Genetics

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“…Microsatellite analysis was performed on a sample of 223 Kazakh Tazy dogs for 18 microsatellite loci recommended by the International Society for Animal Genetics (ISAG) for dogs [30]. The allele frequencies at these loci were used to assess genetic variability within the population (Table 1).…”

Section: Resultsmentioning

confidence: 99%

Advanced median-based genetic similarity analysis in Kazakh Tazy dogs: A novel approach for breed conformity assessment

Perfilyeva,

Mussabayev,

Bespalova

et al. 2024

Preprint

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The breed conformity evaluation is crucial for the preservation of the traits that characterise each dog breed. The use of genetic markers for this purpose provides a precision and objectivity that can surpass the reliability of phenotypic evaluations. In this study, we present a new simple algorithm for assessing breed conformity. The algorithm creates a similarity matrix based on genotypic data and then uses the median to calculate the percentage of genetic similarity that an individual has in relation to the genetic diversity of the breed. To validate the proposed algorithm, we applied it to the genotypic data of 18 microsatellites and 43,691 single nucleotide polymorphisms (SNPs) of the Kazakh Tazy dog, a breed of great cultural and historical importance to Kazakhstan that is now threatened with extinction due to crossbreeding. The algorithm showed a moderate correlation between the microsatellite and SNP genotyping methods, reflecting the different aspects of genetic similarity. In particular, the SNP-based evaluations agreed better with the expert judgements, highlighting their potential for accurate analysis of breed conformity. The proposed algorithm provides easily interpretable results, is flexible, adapts to different genetic markers and may provide an evaluation mechanism for breed conformity in situations where there is no reference population, incomplete pedigrees, unidentified meta-founders and high genetic diversity in the population.Author summaryOur research was initiated by the urgent concern for the possible extinction of the Kazakh Tazy dog, a breed with deep historical roots and cultural significance in Kazakhstan. Information at the DNA level may lead to faster genetic improvement of the breed than relying only on phenotypic data and pedigrees. Genotypic data can be processed to provide valuable insights into genetic diversity, relatedness and ancestry. However, existing methods do not provide a measure of percentage similarity that can be used to assess how closely a particular individual matches the typical genetic composition of the breed. These challenges have led us to propose an approach that overcomes the limitations of existing methods and allows genetic similarity to be assessed based on genotypes. It is based on a median-based approach to analyze genetic data and has been applied to microsatellite and SNP markers but can also be adapted to other genetic markers. This method provides results even in the absence of a defined reference population, complete pedigrees or identified meta-pedigrees and during high genetic diversity within a breed. It can provide breeders and researchers with a tool to maintain the genetic purity of unique breeds.

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The assessment of the efficacy of STRs panels recommended by the ISAG for canine pedigrees analysis for forensic casework

Cited by 2 publications

References 19 publications

A panel of tetranucleotide STR markers as an alternative approach to forensic DNA identification of wolf and dog

A panel of tetranucleotide STR markers as an alternative approach to forensic DNA identification of wolf and dog

Advanced median-based genetic similarity analysis in Kazakh Tazy dogs: A novel approach for breed conformity assessment

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