The assimilation of radio‐active phosphorus deficiency in rats

Gaunt, W. E.; Griffith, H. D.; Irving, J. T.

doi:10.1113/jphysiol.1942.sp003949

Cited by 20 publications

(4 citation statements)

References 9 publications

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“…We derived the minimum urinary excretion rate for N from data presented by Womack et al (1953) on loss of N experienced by rats in negative N balance and the minimum levels of urinary P excretion, from the studies of Gaunt et al (1942) on P-deprived rats. The minimal rates of urinary excretion of N and P are calculated as linear functions of the animal's metabolic rate.…”

Section: Absorption Of Nutrientsmentioning

confidence: 99%

Nutritional Ecology of Microtine Rodents: A Simulation Model of Mineral Nutrition for Brown Lemmings

Barkley¹,

Batzli²,

Collier³

1980

Oikos

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Section: Absorption Of Nutrientsmentioning

confidence: 99%

Nutritional Ecology of Microtine Rodents: A Simulation Model of Mineral Nutrition for Brown Lemmings

Barkley¹,

Batzli²,

Collier³

1980

Oikos

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“…Comparative effect of mineral intake and vitamin D on the forming incisor tooth and bone There is evidence to show that developing teeth and bone react differently to dietary changes in Ca, P and vitamin D (Gaunt & Irving, 1940;Gaunt, Griffith & Irving, 1942;Irving, 1957;McRoberts & Hill, 1962;Ferguson & Hartles, 1964, 1966.…”

Section: Growthmentioning

confidence: 99%

The effect of vitamin D in rats maintained on diets with different mineral content but with the same calcium to phosphorus ratio of unity

Harrand

Hartles

1970

Br J Nutr

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I. Eight groups of ten weanling rats were maintained for 60 d on diets containing calcium and phosphorus in the ratio I : I (w/w) at four different levels of mineral, namely o.08,0*12, 0.24 and 0.36 yo Ca, with and without the addition of ergocalciferol. 2.Provision of vitamin D increased final body-weight except at the highest level of mineral intake. Similarly, increasing the mineral intake increased final body-weight except at the highest level.3. Vitamin D raised the serum Ca concentration at all levels of mineral intake but in the experiment as a whole had no significant effect on serum P concentration. Serum Ca concentration was significantly higher at the two higher levels of mineral intake than at the two lower levels. Increasing dietary mineral caused a progressive increase in serum P concentration, except at the highest level of intake. 4.In general, the addition of vitamin D to the diets had no effect on the fresh or dry weights of the humeri; there was an indication, however, that at the two lower levels of mineral intake, vitamin D reduced the fresh weight of bone. The addition of the vitamin caused a reduction in bone volume, and an increase in bone length, bone ash and the ratio of the weight of ash to the weight of organic matter in dry, fat-free bone. With increasing dietary mineral there was an increase in the value of all the bone measurements except bone volume, which was decreased.5. The addition of vitamin D and increasing dietary mineral both caused an increase in tooth mass and ash content.6. The ratios of tooth mass to bone mass, and tooth ash to bone ash were unaffected by vitamin D, but decreased with increasing mineral intake. 7.Histological examination of the bones showed that the animals receiving 0.08 % of Ca and P were markedly rachitic although the presence of vitamin D ameliorated the condition. When the dietary mineral intake was raised to O * I Z~~ Ca and P the animals were less severely rachitic and in the presence of vitamin D the bones appeared normal.8. The incisor teeth were abnormal at the two lower levels of mineral intake, but when vitamin D was provided, the histological appearance was improved. When the Ca and P level in the diet was 0.24 yo, the incisal dentine appeared normal but the apical predentine was slightlywider thannormal; addition of vitamin D produced teeth of normal appearance. The incisor teeth were normal in appearance in the remaining groups. 9.These results are compared with those obtained in two previous studies where the Ca to P ratios were I : 10 and 10: I (Harrand & Hartles, 1968, 1969).The effect of vitamin D on the bones and teeth of the rat varies with the state of calcium and phosphorus nutrition of the animal. We have reported on the effect of the vitamin at differing levels of mineral intake at two extremes of Ca to P ratio, namely I : 10 and 10 : I by weight (Harrand & Hartles, 1968, 1969). The experiments now reported involved the maintenance of weanling rats on diets with and without vitamin D, at four different levels of dietary mineral...

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“…In the present study the procedure of Gaunt et al (1942) was followed and amplified, determinations of activity being made separately in dentine and enamel of teeth at different stages of development from normal sheep and from sheep in which a deficiency had been induced by feeding diets low in either calcium, phosphorus or phosphorus and vitamin D. Where possible the data on uptake of 32 P was correlated with the clinical appearances of the teeth described in a previous communication (McRoberts, Hill & Dalgarno, 1965a). EXPERIMENTAL Some animals from Exps.…”

mentioning

confidence: 99%

The effects of diets deficient in phosphorus, phosphorus and vitamin D, or calcium, on the skeleton and teeth of the growing sheep IV. The uptake of radioactive phosphorus by the bones and teeth

McRoberts¹,

Hill²,

Dalgarno³

1965

J. Agric. Sci.

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Gaunt, Griffith & Irving (1942) found a 27% greater uptake of 32 P by the incisors of rats fed on a diet containing 0-12% calcium and phosphorus than by the incisors of similar rats fed on a diet containing 0-30 % of these elements, although there was no difference between groups in the percentage of ash of these teeth. The authors concluded that the use of radioactive isotopes in investigating the mineral metabolism of teeth was more sensitive than ash analyses.In the present study the procedure of Gaunt et al. (1942) was followed and amplified, determinations of activity being made separately in dentine and enamel of teeth at different stages of development from normal sheep and from sheep in which a deficiency had been induced by feeding diets low in either calcium, phosphorus or phosphorus and vitamin D. Where possible the data on uptake of 32 P was correlated with the clinical appearances of the teeth described in a previous communication (McRoberts, Hill & Dalgarno, 1965a). EXPERIMENTAL Some animals from Exps. 2 and 3 of a study described previously (McRoberts et al. 1965a) were used in the present study, together with those of a further experiment, Exp. 4. There were four 6-month-old sheep from Exp. 2; two had been fed from weaning at 2 months on a diet low in phosphorus (group C) and two, weaned at the same age, fed on a control diet (group A). From Exp. 3 there were three groups of six 18-month-old sheep; group A had been fed a control diet, group B a diet deficient in phosphorus and vitamin D and group E a diet deficient in calcium for 12 months in each case. In Exp. 4, two groups of six sheep from the same batch as those used for Exp. 3 were treated in the same manner as those of groups A and B of * Present addresses: Hy-Line Poultry Farms, Johnston, Iowa, U.S.A.f Present address: Royal Veterinary College, University of London.Exp. 3, but were killed at 12 months of age compared with 18 months in Exp. 3. A summary of the treatments and the numbers of animals involved is given in Table 1. In Exp. 3, group E animals were given a dietary supplement of calcium carbonate for 2 days prior to injecting with 32 P in order to provide an internal environment suitable for 32 P uptake by new mineralization as well as by exchange.Each animal was given a dose of 32 P as phosphate by intravenous injection; blood samples were taken at 1, 4, 24 and 48 hr., after dosing with 32 P, for the determination of inorganic phosphorus and total 3a P activity, and the animals were killed when the 48 hr. sample was taken. Surface counts were made on the flesh-free radii, mandible and cervical vertebrae for Exps. 3 and 4, using a thin, mica endwindow counter. Samples of bones and teeth were prepared for determinations of activity by boiling in a 3 % potassium hydroxide solution in ethylene glycol, washing in tsopropyl alcohol, drying and dissolving the mineral fraction remaining in 3N-HC1. Separate samples of dentine and enamel were prepared from certain teeth of Exps. 3 and 4 by the method of Manly & Hodge (1939). The activities ...

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The assimilation of radio‐active phosphorus deficiency in rats

Cited by 20 publications

References 9 publications

Nutritional Ecology of Microtine Rodents: A Simulation Model of Mineral Nutrition for Brown Lemmings

Nutritional Ecology of Microtine Rodents: A Simulation Model of Mineral Nutrition for Brown Lemmings

The effect of vitamin D in rats maintained on diets with different mineral content but with the same calcium to phosphorus ratio of unity

The effects of diets deficient in phosphorus, phosphorus and vitamin D, or calcium, on the skeleton and teeth of the growing sheep IV. The uptake of radioactive phosphorus by the bones and teeth

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