The Association Among Biomarkers of Renal and Heart Function in Patients With Heart Failure: the Role of NGAL

Oikonomou, Evangelos; Tsalamandris, Sotiris; Karlis, Dimitris; Siasos, Gerasimos; Chrysοhoou, Christina; Vogiatzi, Georgia; Dimitropoulos, Stathis; Charalambous, Georgios; Kouskouni, Evangelia; Tousoulis, Dimitris

doi:10.2217/bmm-2018-0100

Cited by 9 publications

(9 citation statements)

References 31 publications

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“…By MTT proliferation, wound healing and transwell assays, it was found that overexpression NGAL in NPC cells inhibited the proliferation and migration of NPC cells. These results suggest NGAL plays a cancer suppressor role in NPC, which is different from most solid tumors (12,15). Overexpression of NGAL also induces moderate apoptosis and further study demonstrated that overexpression of NGAL inhibited the caspase family activation.…”

Section: Discussionmentioning

confidence: 83%

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NGAL protects in nasopharyngeal carcinoma by inducing apoptosis and blocking epithelial‑mesenchymal transition

et al. 2020

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In recent years, neutrophil gelatinase-associated lipocalin (NGAL) has been considered to be a key molecule in different cancer types and its carcinogenesis may be related to the NGAL/MMP-9 complex. However, its expression pattern and role in nasopharyngeal carcinoma (NPC) has rarely been reported. In the current study, 158 tumor tissues from NPC patients were collected and immunohistochemistry was performed to determine the NGAL protein expression, to investigate the correlation between its expression and clinical and pathological parameters using Chi square analysis. Furthermore, by over-expressing NGAL in NPC cell lines, biological alteration of NPC cells with respect to cell proliferation, migration and invasion was analyzed. Results suggested that high expression of NGAL predicts better prognosis and longer survival. Overexpression of NGAL significantly reduced the proliferation and migration of NPC cells, and induced the apoptosis by activating caspase 3, 8 and 9, and blocking epithelial-mesenchymal transition by inhibiting mothers against decapentaplegic homolog 2/3 phosphorylation.

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Section: Discussionmentioning

confidence: 83%

“…NGAL, also known as lipocalin-2 (lcn2), is a 24 kDa glycoprotein in humans encoded by the lcn2 gene located at position 3P11 of chromosome 9. In recent years, it has become a biomarker for some benign and malignant diseases (13)(14)(15)(16)(17). The effect of NGAL in carcinogenesis is dependent on cancer type.…”

Section: Introductionmentioning

confidence: 99%

NGAL protects in nasopharyngeal carcinoma by inducing apoptosis and blocking epithelial‑mesenchymal transition

et al. 2020

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“…Neutrophil gelatinase-associated lipocalin (NGAL), also known as lipocalin-2 protein, is a 25-kDa protein involved in the host defense against some gram-negative bacteria through binding with iron-loaded bacterial siderophores, which limits the availability of this essential nutrient to bacteria, leading to inhibition of their growth and pathogenicity [5]. NGAL levels have been used to reflect tissue damage, particularly in the kidney, and more recently for cardiovascular disease manifestations, including hypertensive cardiac hypertrophy [6], coronary artery disease [7], and acute heart failure [8]. Oikonomou et al reported a positive correlation between NGAL and myocardial fibrosis and remodeling in heart failure patients [8].…”

Section: Introductionmentioning

confidence: 99%

“…NGAL levels have been used to reflect tissue damage, particularly in the kidney, and more recently for cardiovascular disease manifestations, including hypertensive cardiac hypertrophy [6], coronary artery disease [7], and acute heart failure [8]. Oikonomou et al reported a positive correlation between NGAL and myocardial fibrosis and remodeling in heart failure patients [8]. Shalenkova et al found that NGAL may be used as a marker for severity of cardiovascular conditions and heart remodeling in patients after exacerbation of ischemic heart disease [9].…”

Section: Introductionmentioning

confidence: 99%

Neutrophil Gelatinase-Associated Lipocalin2 Exaggerates Cardiomyocyte Hypoxia Injury by Inhibiting Integrin β3 Signaling

Geng

et al. 2019

Med Sci Monit

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Background The neutrophil inflammatory protein, lipocalin-2 (NGAL), is elevated in certain forms of cardiac hypertrophy and acute heart failure. However, the specific role of NGAL in cardiac hypoxia injury is unclear. This study aimed to elucidate the functional role of NGAL in cardiomyocyte hypoxia injury. Material/Methods Neonatal rat cardiomyocytes were transfected with adenovirus [(Ad-NGAL] to overexpress human-NGAL and then were exposed to hypoxia for 24 h to establish a hypoxia model. Cell inflammation was detected by RT-PCT and ELISA assay. Cell apoptosis was detected by TUNEL assay. Oxidative stress was also detected by commercial kits. Results An increased inflammatory response, apoptosis, and augmented oxidative stress were observed after exposure to hypoxia, while NGAL overexpression in cells increased the expression and release of inflammatory cytokines. NGAL overexpression also increased the number of apoptotic cells and the imbalance of Bax/Bcl-2 protein expression. Moreover, NGAL overexpression increased the levels of reactive oxygen species and oxidase activity, but reduced anti-oxidase activity. Mechanistically, we found that NGAL decreased the expression of integrin β3, but not the expression of integrin avβ3 and avβ5, thus inhibiting the downstream protein AKT. When we used the constitutively activated AKT overexpression adenovirus to activate AKT, the deteriorated phenotype by NGAL was counteracted. Conclusions NGAL can directly affect cardiomyocytes and cause cardiomyocyte deteriorated hypoxia injury through inhibiting integrin β3 signaling.

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“…In patients with CHF, an increase in all-cause mortality and hospitalization have been associated with NGAL activation in cardiomyocytes and an increase in its level [5].…”

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confidence: 99%

The relationship between systolic function and serum NGAL levels in patients with chronic heart failure of ischemic origin

Lysenko

Syvolap

Potapenko

2021

ZMJ

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Neutrophil gelatinase-associated lipocalin (NGAL) is considered one of the most informative biomarkers of chronic kidney disease (CKD). NGAL can also serve as a biomarker of cardiovascular disease and heart failure (HF). However, the relationship between systolic function and serum NGAL concentrations in patients with chronic HF (CHF) of ischemic origin remains insufficiently studied. The aim. To study the influence of tubulo-interstitial injury marker NGAL on systolic function in patients with CHF of ischemic origin. Materials and methods. The study included 51 patients with CHF, stage II AB, NYHA II-IV FC. Doppler echocardiographic examination was performed on the device Esaote MyLab Eight (Italy) according to standard methods. NGAL levels were analyzed using an ELISA kit (E-EL-H0096, Elabscience, USA). Depending to the concentration of serum NGAL, the patients were divided into 2 subgroups. In the first group (n = 37), the NGAL level was higher than 168 ng/ml, in the second (n = 14) – less than 168 ng/ml. Results. The mean serum NGAL concentration in the first subgroup was 192 (183; 200) ng/ml, in the second subgroup – 154 (134; 160) ng/ml. The patients with CHF of ischemic origin with tubulo-interstitial injury (according to the serum concentration of NGAL) did not differ significantly from the patients with CHF of ischemic origin without tubulo-interstitial injury in age (P = 0.950), height (P = 0.983), weight (P = 0.681), body surface area (P = 0.975). Most of left ventricular systolic function indicators showed a downward tendency (S 6.90 ± 2.85 cm/s vs. 7.67 ± 2.83 cm/s (P = 0.536); S lat 7.33 ± 2.08 cm/s vs. 11.00 ± 4.00 cm/s (P = 0.467); TEI LV 0.56 ± 0.26 c.u. vs. 0.49 ± 0.14 c.u. (P = 0.747)) in the patients with CHF of ischemic origin with elevated serum levels of NGAL compared to similar indicators in the patients with CHF of ischemic origin without tubulo-interstitial injury. The index of LVEF was significantly lower in the patients with CHF with elevated serum NGAL compared to that in the patients with CHF with normal serum NGAL (50.43 ± 17.85 % vs. 63.29 ± 13.24 % (P = 0.021)). Conclusions. Serum NGAL was not only the sensitive marker of tubulo-interstitial injury in patients with CHF of ischemic origin, but also appeared to be a predictor of changes in systolic heart function.

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The Association Among Biomarkers of Renal and Heart Function in Patients With Heart Failure: the Role of NGAL

Cited by 9 publications

References 31 publications

NGAL protects in nasopharyngeal carcinoma by inducing apoptosis and blocking epithelial‑mesenchymal transition

NGAL protects in nasopharyngeal carcinoma by inducing apoptosis and blocking epithelial‑mesenchymal transition

Neutrophil Gelatinase-Associated Lipocalin2 Exaggerates Cardiomyocyte Hypoxia Injury by Inhibiting Integrin β3 Signaling

The relationship between systolic function and serum NGAL levels in patients with chronic heart failure of ischemic origin

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