2018
DOI: 10.1016/j.smallrumres.2018.01.016
|View full text |Cite
|
Sign up to set email alerts
|

The association between 171 K polymorphism and resistance against scrapie affection in Greek dairy sheep

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1

Citation Types

0
6
0

Year Published

2018
2018
2021
2021

Publication Types

Select...
5

Relationship

1
4

Authors

Journals

citations
Cited by 5 publications
(6 citation statements)
references
References 34 publications
0
6
0
Order By: Relevance
“…Real-Time PCR is fast, sensitive and suitable for SNP detection. This method has been also used in sheep for the detection of polymorphisms at codons 136, 154 and 171 in the PRNP gene [ 37 ]. Each SNP Genotyping Assay consisted of a mix of sequence-specific forward and reverse primers to amplify the polymorphic sequence of interest and two TaqMan ® MGB (minor groove binder) probes, FAM (6-carboxyfluorescein) and VIC (2′-chloro-7′phenyl-1,4-dichloro-6-carboxy-fluorescein) dye-labeled to detect the amplified product ( Table 1 ).…”
Section: Methodsmentioning
confidence: 99%
“…Real-Time PCR is fast, sensitive and suitable for SNP detection. This method has been also used in sheep for the detection of polymorphisms at codons 136, 154 and 171 in the PRNP gene [ 37 ]. Each SNP Genotyping Assay consisted of a mix of sequence-specific forward and reverse primers to amplify the polymorphic sequence of interest and two TaqMan ® MGB (minor groove binder) probes, FAM (6-carboxyfluorescein) and VIC (2′-chloro-7′phenyl-1,4-dichloro-6-carboxy-fluorescein) dye-labeled to detect the amplified product ( Table 1 ).…”
Section: Methodsmentioning
confidence: 99%
“…The authors reported that K171 was the second most resistant allele after arginine (R > K > H > Q). 6 Evaluation of IHC-stained tissues from IC-inoculated sheep revealed intergenotypic differences in PrP Sc phenotypes (pattern and distribution). White matter immunolabeling was largely present in the KK and QK genotype, contrasting a relative absence in sheep with the QQ genotype.…”
Section: Discussionmentioning
confidence: 99%
“…14,21,32 However, little is known about the contribution of lysine (K) other than the presence of naturally occurring scrapie affecting heterozygous K171 sheep in Greek and Italian sheep. 1,6 K171 has a relatively higher incidence in some Mediterranean breeds and also occurs in other breeds seen in the United States, including Barbado, Barbado/St Croix, Dorper, and Suffolk crosses. 2,17 Nonrandom sampling of flocks in the United States has also identified the polymorphism in American Blackbelly, Black Hawaiian, Painted Desert, and Texas Dall sheep (D. Sutton and D. Norden, USDA APHIS, personal communication, unpublished data, 2017).…”
mentioning
confidence: 99%
See 1 more Smart Citation
“…Despite these three codons having a strong association with CS, codon 171 has a greater influence compared to the other two codons, where homozygous QQ sheep are susceptible to scrapie and the RR genotype confers resistance ( Table 1 ) [ 13 ]. In 2003, a novel variation was detected in codon 171 (lysine-K) by Guo et al (2003) [ 52 ] and later, Boukouvala et al (2018) [ 53 ] reported that dairy sheep with K171 showed a 75% reduction in the risk of developing scrapie when compared with individuals with Q171. Recently, Cassmann et al (2019) [ 14 ] showed that individuals homozygous for K171 (ARK/ARK) were resistant to CS after oronasal inoculation with PrP Sc .…”
Section: Neuropathologymentioning
confidence: 99%