The escalation of anthropogenic heat emissions poses a significant threat to the urban thermal environment as cities continue to develop. However, the impact of urban spatial form on anthropogenic heat flux (AHF) in different urban functional zones (UFZ) has received limited attention. In this study, we employed the energy inventory method and remotely sensed technology to estimate AHF in Beijing’s central area and utilized the random forest algorithm for UFZ classification. Subsequently, linear fitting models were developed to analyze the relationship between AHF and urban spatial form indicators across diverse UFZ. The results show that the overall accuracy of the classification was determined to be 87.2%, with a Kappa coefficient of 0.8377, indicating a high level of agreement with the actual situation. The business/commercial zone exhibited the highest average AHF value of 33.13 W m−2 and the maximum AHF value of 338.07 W m−2 among the six land functional zones, indicating that business and commercial areas are the primary sources of anthropogenic heat emissions. The findings reveal substantial variations in the influence of urban spatial form on AHF across different UFZ. Consequently, distinct spatial form control requirements and tailored design strategies are essential for each UFZ. This research highlights the significance of considering urban spatial form in mitigating anthropogenic heat emissions and emphasizes the need for customized planning and renewal approaches in diverse UFZ.