The Association between Micro-RNA Gene Polymorphisms and Development of Hepatocellular Carcinoma in Egyptian Patients

Abstract

IntroductionThe development and progression of hepatocellular carcinoma (HCC) is a multistage process involving the deregulation of genes that are crucial to cellular processes. Multiple risk factors are correlated with HCC. MicroRNA is differentially expressed in development of different types of malignancies, including hepatic malignancy. Single nucleotide polymorphisms (SNPs) are the most common sequence variation in human genome. SNPs in miRNAs may affect transcription, processing or target recognition and result in malignant diseases. Aim: To determine the association between micro-RNA gene polymorphisms and development of HCC in Egyptian Patients.Material and methodsThis study included 200 individual who were matched in age and sex. Tumor staging was done using BCLC staging system. Quantification and genotyping of Micro-RNA were performed.ResultsAmong the 200 patients, 2 groups were described: group I included 90 HCC patients with a male majority (72.2%) and 110 controls in group II. Diabetes and hypertension were detected as risk factors. Three microRNA SNPs were assayed. There was a significant association between rs10061133 miR-499b and the risk of HCC. The genotypes GG or G allele were associated significantly to an increased risk of HCC (GG: OR (95% CI) = 2.91 (1.23-4.22), p = 0.013; G allele: OR (95% CI) = 1.79 (1.12- 2.15), p = 0.026) compared with the genotype of AA or AG or A allele.ConclusionsThere is an association between the mi-RNA SNPs and the susceptibility to HCC, aiming to explore some roles and mechanisms of SNPs within miRNAs in the occurrence and development of HCC.

Red cell distribution width as a predictor of outcome in hospitalized cirrhotic patients

“…(1) The patients in our study were part of a single-center study. (2) We had some missing data such as iron or vitamin B 12 deficiency and other diseases that may affect RCDW values. (3) We analyzed all hospitalized patients together despite their etiologies.…”

“…Most causes of morbidity and mortality in chronic hepatitis patients are related to liver fibrosis, liver cirrhosis and portal hypertension [1][2][3]. Hepatitis C virus (HCV) infection is a severe public health issue that affects 170 million individuals globally [4,5].…”

Hepatocellular Carcinoma Incidence and Risk Factors: Insights into the Significance of Genetic Heterogeneity

“…Consequently, genetic differences in miRNAs have been related to the development of HCCs. Numerous studies have shown that susceptibility genes and abnormal gene expression are risk factors for HCC (77).…”

“…At least 12 kinds of cancer, including HCC, have been linked to variations in the TLR4 gene (81). In another study, miRNA499SNPs were shown to decrease the risk of HCC; thus, researchers set out to learn more about the function of miRNAs and the processes by which they contribute to the formation of primary liver cancer (82). The development of HCC is believed to be influenced by various factors, including persistent infections caused by hepatitis B or hepatitis C viruses, exposure to carcinogenic substances, cirrhosis, and multiple SNPs (83).…”

The TM6SF2 variant as a risk factor for hepatocellular carcinoma development in chronic liver disease patients

“…Unfortunately, most HCC patients in Egypt are first diagnosed in the intermediate or advanced stages [1]. For early diagnosis and to reduce HCC-related mortality, new HCC diagnostic and prognostic biomarkers are crucial [2]. Multiple risk factors are correlated with HCC, including alcohol abuse, hepatitis B virus (HBV) or hepatitis C virus (HCV) infection, aflatoxin exposure, nonalcoholic steatohepatitis, and metabolic diseases [2].…”