The association between RASSF1 gene polymorphisms and lung cancer susceptibility among people in Hubei Province of China

Abstract

The relationship between Ala/Ser polymorphism in 133 codon of exon 3 region of the RASSF1 gene and genetic susceptibility of lung cancer in Hubei province Han population was investigated by a case-control study. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was adopted to analyze the polymorphism of codon 133 of exon 3 in the RASSF1 gene of 100 pathologically diagnosed lung cancer patients, and 100 healthy controls. The relationship between different genotypes and the susceptibility of lung cancer was analyzed. Among 200 blood samples from Han people in Hubei Province, including 100 from lung cancer patients and 100 from healthy controls, the frequencies of Ala/Ala, Ala/Ser, Ser/Ser genotype of the RASSF1 in lung cancer patients were 83%, 16%, 1%, and those in healthy controls was 93%, 7%, 0% respectively, with the difference being statistically significant between two groups (P<0.05). The individuals with Ala/Ser genotype had higher risk of suffering from lung cancer, with an OR of 2.341, and 95% CI of 1.009-6.393 respectively. It was concluded that RASSF1Ala133Ser was a susceptible genetic factor of lung cancer. Ala/Ser genotype increased the risk of lung cancer.

Species and Life‐Stage Sensitivity of Chinese Rare Minnow (Gobiocypris rarus) to Chemical Exposure: A Critical Review

“…Mechanistic toxicity studies revealed that heavy metals such as cadmium, copper, and lanthanum could damage liver and gill tissue and act as antioxidants (Yin 2014; Zhang et al 2014; Jiang et al 2015a; Hua 2017). In the case of biocides, it is known that cypermethrin, fenvalerate, and chlorpyrifos damage the nervous system of various fish species, causing loss of equilibrium that may lead to death (Cao 2010; Jiang et al 2015b; Wang 2017). However, those neurotoxicants lose their specific activity in embryos and larvae with immature nervous systems (Yamagami 1981; Sobanska et al 2018).…”

Association between RASSF1A Ala133Ser Polymorphism and Cancer Susceptibility: A Meta-Analysis Involving 8,892 Subjects

“…Thus, a total of 14 case-control studies including 4572 cases and 4320 controls were analyzed in this meta-analysis (Schagdarsurengin et al, 2005;Kanzaki et al, 2006;Gao et. al, 2008;Xiao et al 2009;Bergqvist et al, 2010;Donninger et al, 2011;Bayram, 2012;Kawai et al 2012;Meyer et al, 2013;Zhou et al, 2013). The characteristics of selected studies are summarized in Table 1.…”

“…The sample size in these case-control studies varied considerably (range . All studies were case-control studies, including four breast cancer studies (Schagdarsurengin et al, 2005;Gao et al, 2008;Bergqvist et al, 2010;Donninger et al, 2011), two lung cancer studies (Kanzaki et al, 2006;Xiao et al 2009), two esophageal squamous cell carcinoma (ESCC) studies (Kanzaki et al, 2006;Zhou et al, 2013), and the others including colorectal cancer (Kanzaki et al, 2006), head and neck cancer (Kanzaki et al, 2006), renal cell carcinoma (Kawai et al 2012), hepatocellular carcinoma (Bayram, 2012), gastric cancer (Zhou et al, 2013), prostate cancer (Meyer et al, 2013). 8 studies conducted in the Asian population, 5 in Caucasian population, and 1 in mixed population.…”

The Association of Ala133Ser Polymorphism and Methylation in Ras Association Domain Family 1A Gene With Unfavorable Prognosis of Hepatocellular Carcinoma

“…The most widespread form of human genetic variation is single nucleotide polymorphism (SNP), which may contribute to tumor sensitivity. To date, many studies have demonstrated that SNPs of RASSF1A are associated with the risk of many types of cancers, including breast cancer ( 12 - 15 ), lung cancer ( 16 , 17 ), esophageal cancer ( 16 , 18 ), head and neck cancer ( 16 ), colorectal cancer ( 16 ), renal cell carcinoma ( 19 ), HCC ( 20 ), gastric cancer ( 18 ), and prostate cancer ( 21 ). It has been proven that guanine (G)/thymine (T) SNP at the first position of codon 133 in exon 3 of RASSF1A (dbSNP ID: rs2073498), leads to the replacement of an Ala residue (GCT) through the Ser residue (TCT) in the ataxia telangiectasia mutated (ATM) phosphorylated position, and can impact the function of RASSF1A ( 22 , 23 ).…”