The Association of Bovine Osteopontin (OPN) Gene with Milk Production Traits in Iranian Holstein Bulls

Salehi, Abdolreza; Nasiri, Khadijeh; Aminafshar, Mehdi; Sayaadnejad, Mohammad Bagher; Sobhani, Rohoallah

doi:10.15171/ijb.1092

Cited by 10 publications

(12 citation statements)

References 20 publications

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“…Similarly, Boleckova et al [20] also could not find any significant effect for milk yield and fat yield in case of OPN gene variants in Czech Fleckvieh cows. However, the present observations are against the reports of lower milk yield associated with C allele compared with T allele [18,25,26,27].…”

Section: Association Analysiscontrasting

confidence: 97%

“…In the present study, no significant association was found out for FP (P = 0.71). However, the previous association studies in cattle [16,18,26] explained that C allele was associated with higher milk fat percentage. Any reports regarding the effect of g.8514C > T polymorphism with SNFP or LACP in milk were found out to compare with the present study.…”

Section: Association Analysismentioning

confidence: 82%

“…In agreement with the present results, Łuczak and Hanna [22] and Kulaj et al [25] also could not derive any significant results for g.8514C > T polymorphism with regard to milk composition traits in Jersey cattle. In contrast, the influence of C allele on protein content was explained in CDDR Holstein [16,18] and Iranian Holstein [20,26,27] populations where significantly higher protein percent was noticed in CC genotyped animals. In the present study, no significant association was found out for FP (P = 0.71).…”

Section: Association Analysismentioning

confidence: 82%

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Effect of Osteopontin gene variants on milk production traits in Holstein Friesian crossbred cattle of Kerala

Lali¹,

Anilkumar²,

Aravindakshan³

2020

Turk J Vet Anim Sci

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Osteopontin gene (OPN) is located in the quantitative trait loci (QTL) for milk production traits in bovine chromosome 6. In the present study the impact of cytosine to thymine transition in the intron-4 of OPN gene (g.8514C > T) on milk production traits was analysed in the Holstein Friesian crossbred cattle of Kerala. Genomic DNA was isolated and a fragment of 290 bp enclosing the polymorphic site was amplified and genotyped by restriction fragment length polymorphism (RFLP) using endonuclease, BSeN1. The genetic variants were distributed according to Hardy-Weinberg equilibrium. The T allele was found to be the major one (T/0.76, C/0.24) and the genotype frequencies were TT/0.60, CT/0.32, and CC/0.08. The amplicons of genotypes were sequenced by sangers dideoxy termination technique and confirmed the mutation. Association study using the General Linear Model-Analysis of Variance (GLM-ANOVA) considering marker, season of calving, parity and herd as fixed factors and dairy trait as dependent variable revealed that none of the yield traits (305 day milk yield, peak yield, fat yield, protein yield, solids not fat yield, lactose yield, daily milk yield) or composition traits (fat percent, protein percent, solids not fat percent and lactose percent) of milk production analysed were significantly differed between CT and TT genotyped animals. The OPN gene polymorphism (g.8514C > T) can be suggested for marker assisted selection (MAS) for future breeding programmes for dairy cattle only after extensive association studies.

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Section: Association Analysiscontrasting

confidence: 97%

Section: Association Analysismentioning

confidence: 82%

Section: Association Analysismentioning

confidence: 82%

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Effect of Osteopontin gene variants on milk production traits in Holstein Friesian crossbred cattle of Kerala

Lali¹,

Anilkumar²,

Aravindakshan³

2020

Turk J Vet Anim Sci

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“…It was shown that acute and chronic inflammations are associated with greater expression of OPN on T cells, macrophages, NK and dendritic cells, and an increase in the OPN level in bodily fluids (O'Regan et al 1999;O'Regan and Berman, 2000). This protein is found in blood, milk, urine, bile and semen (Salehi et al 2015). It was also determined that OPN is involved in processes such as angiogenesis, cell adhesion, apoptosis, inflammation regulation and wound healing (Denhardt et al 2001), regulation of growth and foetal development, and maintenance of pregnancy to full term (Johnson et al 2003;Leonard et al 2005), and the development of bovine in-vitro embryos (Monaco et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Effects of the c.8514C > T polymorphism in the osteopontin gene (OPN) on milk production, milk composition and disease susceptibility in Holstein-Friesian cattle

Kułaj

Pokorska

Ochrem

et al. 2019

Italian Journal of Animal Science

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Studies into the polymorphism of the bovine osteopontin (OPN) gene have so far been focussed on the association between milk production and milk composition, lactation persistency and the rate of growth and weight gain in young cattle. Results obtained by some authors have shown different associations for various milk parameters. Even though osteopontin is an essential component of immunological response, the relationship between the polymorphism of the osteopontin gene and the incidence of diseases in cows has not been researched as yet. The purpose of this study was to analyse the c.8514 C > T polymorphism and its association with milk parameters such as: average daily yield, average protein and fat percentage and average somatic cell count in Holstein-Friesian cattle. The study also included an analysis of the impact of polymorphism on the incidence of the most frequent cattle diseases such as: clinical mastitis (CM), ovarian cysts (OC), clinical hypocalcaemia (CH) and pyometra (P). The study population of cows was found to have two alleles in the analysed locus of the osteopontin gene: C (0.529) and T (0.471), and three genotypes: CC, CT and TT (0.288, 0.482 and 0.230, respectively). Although the study showed no significant association between the polymorphism of the OPN gene and the incidence of diseases or milk parameters, it is an important contribution to research aimed at identifying the most essential SNPs which could be used for marker-assisted selection in dairy cattle herds. HIGHLIGHTS The study population was found to have two alleles in the locus of the osteopontin gene (c.8514C>T): C and T, and three genotypes: CC, CT and TT. CT was the most frequent genotype of the osteopontin gene (c.8514C>T), identified in about 48% of the study population of cows. No significant differences were focused between the genotypes in the c.8514C>T locus and the milk parameters and the incidence of cattle diseases.

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“…No associations were observed between the singlenucleotide polymorphisms in analysed loci and the milk performance traits; however, the findings showed some tendencies towards reaching lower or higher values of a given trait by an individual of a given genotype. Salehi, et al, [31] reported association of bovine osteopontin (OPN) gene with milk production traits in Iranian Holstein Bulls. A total of 100 Semen samples from progeny tested Iranian Holstein bull's were collected.…”

Section: Association Studies With Different Traits Production Traitmentioning

confidence: 99%

Role of Osteopontin in Bovines and their Association with Different Traits: A Review

Kumar¹,

Mukherjee²,

Kumar³

et al. 2018

biop

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The Association of Bovine Osteopontin (OPN) Gene with Milk Production Traits in Iranian Holstein Bulls

Cited by 10 publications

References 20 publications

Effect of Osteopontin gene variants on milk production traits in Holstein Friesian crossbred cattle of Kerala

Effect of Osteopontin gene variants on milk production traits in Holstein Friesian crossbred cattle of Kerala

Effects of the c.8514C > T polymorphism in the osteopontin gene (OPN) on milk production, milk composition and disease susceptibility in Holstein-Friesian cattle

Role of Osteopontin in Bovines and their Association with Different Traits: A Review

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