The Association of Bovine PPARGC1A and OPN Genes with Milk Composition in Two Independent Holstein Cattle Populations

Khatib, Hasan; Zaitoun, Ismail; Wiebelhaus-Finger, J.; Chang, YM; Rosa, Guilherme J. M.

doi:10.3168/jds.2006-812

Cited by 90 publications

(105 citation statements)

References 15 publications

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“…Weikard et al (2005) showed that SNP T>C of PPARGC1A gene had a significant effect on milk fat yield in the German Holstein population. Khatib et al (2007) reported significant associations between SNP A>C of PPARGC1A gene and milk yield, milk protein percentage, and SCS in the North American Holstein population. Leonard et al (2005) reported significant associations between SNP A>C of OPN gene and milk protein percentage and milk fat percentage in the North American Holstein population.…”

Section: Introductionmentioning

confidence: 99%

“…Many studies have reported that the highest number of these QTL have been located on chromosome 6 (Khatkar et al 2004). Osteopontin (OPN) and peroxisome proliferator activated receptor-γ coactivator-1α (PPARGC1A termed PGC-1α) are located in the middle of chromosome 6 of bovine about 6Mb apart, which is approximately 12 cM (Khatib et al 2007). PPARGC1A is as activator of various nuclear hormone receptors and mediator in expression of genes involved in oxidative metabolism, adipogenesis, and gluconeogenesis.…”

Section: Introductionmentioning

confidence: 99%

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Association of bovine PPARGC1A and OPN genes with milk production and composition in Holstein cattle

Pasandideh¹,

Mohammadabadi²,

Esmailizadeh³

et al. 2015

CZECH J ANIM SCI

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ABSTRACT:Several studies have reported quantitative trait loci on chromosome 6 affecting milk production and composition traits in dairy cattle. Osteopontin (OPN) and peroxisome proliferator activated receptor-γ coactivator-1α (PPARGC1A) genes have been located on this chromosome and identified as positional candidates for milk traits. We investigated the associations of single nucleotide polymorphism (SNP) T>C at position 1892 and SNP A>C at position 3359 in PPARGC1A gene as well as SNP C>T at position 8514 in OPN gene with milk production and composition. Hence, 398 Iranian Holstein cows were genotyped through polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). The Least Squares methods were used to examine the effects of genotypes on milk traits. The frequencies of the C allele at position 1892, A allele at position 3359, and T allele at position 8514 were 0.56, 0.64, and 0.53, respectively. In this study, c.1892T>C genotypes indicated significant associations with milk fat content adjusted for two milkings per day (FAT P2X ; %), estimated breeding value for milk (EBV M ; kg), milk protein yield adjusted for mature body weight (PRO ME ; kg), milk protein yield adjusted for 305 days (PRO 305 ; kg) (P < 0.05), and estimated breeding value for milk fat content (EBV FP ; %) (P < 0.01). There were significant associations between c.3359A>C genotypes and FAT P2X , EBV FP (P < 0.01). Moreover, significant associations were shown between c.8514C>T genotypes in OPN gene and FAT P2X (P < 0.05), and PRO PER305 (P < 0.01). Thus, these SNPs would provide an excellent opportunity for marker assisted selection programs in dairy cattle.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Association of bovine PPARGC1A and OPN genes with milk production and composition in Holstein cattle

Pasandideh¹,

Mohammadabadi²,

Esmailizadeh³

et al. 2015

CZECH J ANIM SCI

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“…Khatib et al (2007) observaram frequências genotípicas do gene OPN de 26, 51 e 23%, respectivamente, para os genótipos TT, CT e CC desse SNP divergindo das frequências genotípicas encontradas nos animais girolando, nos quais houve preponderância do alelo T.…”

Section: Resultsunclassified

“…Posteriormente, essa associação foi confirmada em famílias co-segregantes da raça holandesa da Polônia, onde foi detectado efeito significativo do alelo T do gene OPN com o maior peso corporal em novilhas e touros jovens com 3, 6 e 12 meses de idade (Pareek et al, 2008b). O alelo C desse mesmo SNP (C/T) do gene OPN foi associado ao aumento no percentual de gordura e proteína do leite em duas diferentes populações da raça holandesa (Leonard et al, 2005;Khatib et al, 2007).…”

Section: Resultsunclassified

“…Um polimorfismo de um único nucleotídeo (SNP) C/T no gene OPN localizado no íntron 4 foi associado ao aumento nos percentuais de proteína e gordura no leite, sendo o alelo C responsável por efeito aditivo das características (Leonard et al, 2005). Khatib et al (2007) confirmaram os resultados encontrados por Leonard et al (2005) na raça holandesa, reforçando a importância desse gene na expressão destas características em bovinos leiteiros.…”

Section: Introductionunclassified

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Análise de diversidade genética do gene da osteopontina em bovinos da raça girolando

et al. 2011

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RESUMO -Objetivou-se obter os índices de diversidade genética para o SNP (single nucleotide polymorphism) do íntron 4 do gene da osteopontina (OPN) para 434 animais (87 touros e 347 vacas) participantes do Teste de Progênie da raça girolando no Brasil. Para a amplificação, foram utilizados primers descritos para a raça holandesa, e a diferenciação dos alelos C/T desse SNP foi obtida por meio da técnica de PCR-RFLP. As frequências genotípicas TT (52,53%), CT (38,71%) e CC (8,76%) e as frequências alélicas de T (71,9%) e C (28,1%) indicam que a população encontra-se em Equilíbrio de Hardy-Weinberg (EHW).Apesar de o loco do gene OPN estar em EHW, a frequência superior do alelo T do SNP nesses animais pode sugerir uma tendência de fixação do alelo T na raça. Não foi observada diferenciação entre o grupo de touros e vacas (F ST = -0,018), corroborando a estimativa de equilíbrio da população. Considerando os valores estimados pelo F IS (0,043), é possível que ocorram altos números de indivíduos homozigotos para o alelo T observados na população, em virtude da provável herança desse alelo vindo da raça zebuína, e não a endogamia. Assim, para melhor caracterização do polimorfismo do gene OPN, devem ser realizadas avaliações em maior número de animais, uma vez que só foram avaliados animais participantes do teste de progênie.Palavras-chave: bovinos leiteiros, polimorfismo, produção de leite, SNP, SPP1 Analysis of genetic diversity of osteopontin gene in girolando cattleABSTRACT -The objective was to obtain the indices of genetic diversity for the SNP (single nucleotide polymorphism) of the 4 intron osteopontin gene (OPN) for 434 animals (87 bulls and 347 cows) participants in the Teste de Progênie da raça girolando (Girolando Progeny Test) in Brazil. For amplification, primers used were described for the Holstein breed, and differentiation of alleles C/T SNP that was obtained by PCR-RFLP. Genotype frequencies of TT (52.53%), CT (38.71%) and CC (8.76%) and allele frequencies of T (71.9%) and C (28.1%) indicate that the population is in Hardy-Weinberg principle (HWP). Although the OPN gene locus is in HWP, the higher frequency of allele T of SNP in these animals may suggest a settingtrend of allele T in the race. No difference was observed between the group of bulls and cows (F ST = -0.018), supporting the estimate of population balance. Considering the values estimated by the F IS (0.043), it is likely that high numbers of individuals homozygous for the T allele observed in the population occur because of possible inheritance of this allele coming from the zebu breed, rather than inbreeding. Thus, to better characterize the OPN gene polymorphism, assessments in a larger number of animals must be performed, since only animals that participated in the Progeny Test were assessed.Key Words: dairy cattle, polymorphism, milk production, SNP, SPP1 Revista Brasileira de Zootecnia IntroduçãoAnimais da raça girolando destacam-se no cenário nacional por sua representatividade na produção de leite, pois são responsáveis por 80% da p...

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Effect ofABCG2,PPARGC1A,OLR1 andSCD1 gene polymorphism on estimated breeding values for functional and production traits in Polish Holstein-Friesian bulls

Komisarek

Dorynek

2009

J Appl Genet

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This study investigated the impact of 6 polymorphisms located in the ABCG2, PPARGC1A, OLR1 and SCD1 genes on estimated breeding values for milk production, longevity, somatic cell count and reproductive traits. The analysis was conducted on 453 Polish Holstein-Friesian bulls. Genotypes were identified using PCR-RFLP, and haplotype inferences were performed for 3 linked mutations of PPARGC1A. The most significant associations were found between the A/C polymorphism located in exon 14 of ABCG2 and milk fat production traits as well as calving-to-first insemination interval, and between the T/C substitution in intron 9 of the PPARGC1A and non-return rate in heifers.

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The Association of Bovine PPARGC1A and OPN Genes with Milk Composition in Two Independent Holstein Cattle Populations

Cited by 90 publications

References 15 publications

Association of bovine PPARGC1A and OPN genes with milk production and composition in Holstein cattle

Association of bovine PPARGC1A and OPN genes with milk production and composition in Holstein cattle

Análise de diversidade genética do gene da osteopontina em bovinos da raça girolando

Effect ofABCG2,PPARGC1A,OLR1 andSCD1 gene polymorphism on estimated breeding values for functional and production traits in Polish Holstein-Friesian bulls

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