The association of haplotypes inIGFBP-3gene promoter region and tissue expressions in three pig breeds

Abstract

Bama Xiang pig (BM) and Tibetan mini-pig (TM) are used as experimental animals in China; however, the dwarf molecular mechanisms of these Chinese local pig breeds are unknown. IGFBP-3 affects animal growth, carcass and meat quality. The aim of this study was to identify the polymorphisms in the promoter of the IGFBP-3 and analyse their effect on the IGFBP-3 mRNA expression level in liver and muscle tissues. High-density single-nucleotide polymorphisms (SNPs) (31) and InDels (5) were detected in the promoter of the IGFBP-3 in the BM, TM and Junmu No. 1 White (JM, control) pig breeds from 114 individuals by re-sequencing. A perfect Linkage disequilibrium consisted of 13 SNPs was observed in the promoter region and 2 main haplotypes were identified, of which the h1 genotype (GCA-ATGTACATAT) was more prevalent in JM breed than in TM or BM breeds (P < .0001), h2 (ATGTGCACG--CGC) was the dominant haplotype in TM and BM breeds (P < .0001). Expression analysis showed that haplotype of the promoter region is highly associated with the IGFBP-3 mRNA expression level in liver and muscle tissues of pigs. The IGFBP-3 mRNA expression level was determined higher in the liver and muscle tissues of pigs with h2 genotype as compared to that in pigs with h1 genotype (P < .05). The results suggest that the SNPs and haplotypes in the promoter of the IGFBP-3 gene may serve as useful molecular markers for the body growth traits and the breeding in swine.ARTICLE HISTORY

High Altitude Adaptability and Meat Quality in Tibetan Pigs: A Reference for Local Pork Processing and Genetic Improvement

“…The differences in body composition among the different breeds were largely expected due to the breed itself and the different rearing conditions. The three pig breeds from low, intermediate, and high altitudes interestingly exhibited not only different body types, but different breeding intensities as well [13]. A workhorse of the pork industry, YP undergoes high-intensity modern breeding, whereas QYP is subjected to medium-intensity traditional breeding, and TP is bred at a low-intensity and is raised in a semi-wild state.…”

Non-synonymous polymorphism in IGFBP-3 gene associated with growth traits in striped catfish (Pangasianodon hypophthalmus, Sauvage, 1878)

“…The result proved that the non-synonymous SNP 704 C>G (p.Leu8Val) is significantly associated with the growth traits. In previous studies, the associations between SNPs in IGFBP-3 gene and the growth of human (van der Kaay et al, 2009), swine (Wu et al, 2016), common carp (Mehrabi et al, 2015) and chicken (Ou et al, 2009) were found at loci in non-coding region such as promoter, intron and 5'-UTR. In this study, we firstly reported a non-synonymous SNP of IGFBP-3 gene in P. hypophthalmus, which was associated with growth traits of this fish.…”