School bullying is on the rise around the world. The phenomenon of school bullying is a microcosm of the social environment, which means that the bullying phenomenon in the school can be magnified to the whole society, inducing and presenting as a crime, so it must be studied, controlled and prevented. In this paper, horizontal and vertical comparisons were made in the three dimensions of bullies, victims and irrelevant personnel, so as to study and analyze the psychological status of victims by means of scale measurement, and then reveal the relationship between victims and their own anti-social personality. Students from three different middle schools in X city, S Province were randomly selected as the research objects, who had finished the questionnaires of Middle School Bullying Questionnaire, Chinese Big Five Personality Inventory Brief Version, and Anti-social Screening Questionnaire Self-evaluation version. The current bullying situations were obtained through data analysis; significant differences were found in the dimensions of neuroticism, conscientiousness, agreeableness, and extraversion among bullies, victims, and non-bullied; therefore, it was concluded that the bullying victims and their anti-social behavior tendencies were in positive correlation; school bullying had severe impacts on the social activities and personality formations of the victims. The results have provided the theoretical basis for the research on personality building of adolescent victims of school bullying.