The Association of Serum TNF-α Levels and Blood Multi-Elements Modified by TNF-α Gene Polymorphisms in Metal Industrial Workers

Chen, Tzu-Hua; Huang, Joh-Jong; Kung, Wei-Shyang; Lee, Su-Shin; Sun, Hung-Yu; Chuang, Hung-Yi

doi:10.3390/ijerph16214079

Cited by 7 publications

(7 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Data on how rs1800630 affects TNF gene expression levels in the hippocampus taken from the eQTL database were consistent with the previously mentioned findings in the existing literature in that the C allele upregulates TNFα ( Chen et al., 2019 ) in comparison to the homozygous genotype including the alternative allele.…”

Section: Variants With Demonstrated Associations With the Risk Of Neurological Or Psychiatric Diseasessupporting

confidence: 87%

“…(2012) C allele was associated with COX2 overexpression by disrupting micro-RNA-mediated mRNA degradation (not replicated by Piranda et al. (2017) ) rs1800630 (= -863C/A) TNF = LTA Chronic inflammatory response, neurogenesis CC (homozygous reference) Chen et al. (2019) CC genotype was associated with higher serum TNFα levels Higuchi et al.…”

Section: Resultsmentioning

confidence: 99%

“…For the first one, rs1800630, the HPC0A07/03C cells are homozygous for the reference allele (CC). This genotype has been related both to higher serum TNFα levels in response to toxic metal exposure ( Chen et al., 2019 ) and to decreased transcriptional promoter activity following concanavalin A exposure ( Higuchi et al., 1998 ). Furthermore, the C allele of this SNP has been implicated in increased risk of AD ( Tsutsumi et al., 2007 ).…”

Section: Variants With Demonstrated Effects On Gene or Cellular Level As Well As Associations With The Risk Of Neurological Or Psychiatrimentioning

confidence: 99%

See 2 more Smart Citations

The effects of genotype on inflammatory response in hippocampal progenitor cells: A computational approach

Lee

Metz

McDiarmid

et al. 2021

Brain, Behavior, & Immunity - Health

View full text Add to dashboard Cite

Cell culture models are valuable tools to study biological mechanisms underlying health and disease in a controlled environment. Although their genotype influences their phenotype, subtle genetic variations in cell lines are rarely characterised and taken into account for in vitro studies. To investigate how the genetic makeup of a cell line might affect the cellular response to inflammation, we characterised the single nucleotide variants (SNPs) relevant to inflammation-related genes in an established hippocampal progenitor cell line (HPC0A07/03C) that is frequently used as an in vitro model for hippocampal neurogenesis (HN). SNPs were identified using a genotyping array, and genes associated with chronic inflammatory and neuroinflammatory response gene ontology terms were retrieved using the AmiGO application. SNPs associated with these genes were then extracted from the genotyping dataset, for which a literature search was conducted, yielding relevant research articles for a total of 17 SNPs. Of these variants, 10 were found to potentially affect hippocampal neurogenesis whereby a majority (n=7) is likely to reduce neurogenesis under inflammatory conditions. Taken together, the existing literature seems to suggest that all stages of hippocampal neurogenesis could be negatively affected due to the genetic makeup in HPC0A07/03C cells under inflammation. Additional experiments will be needed to validate these specific findings in a laboratory setting. However, this computational approach already confirms that in vitro studies in general should control for cell lines subtle genetic variations which could mask or exacerbate findings.

show abstract

Section: Variants With Demonstrated Associations With the Risk Of Neurological Or Psychiatric Diseasessupporting

confidence: 87%

Section: Resultsmentioning

confidence: 99%

Section: Variants With Demonstrated Effects On Gene or Cellular Level As Well As Associations With The Risk Of Neurological Or Psychiatrimentioning

confidence: 99%

See 1 more Smart Citation

The effects of genotype on inflammatory response in hippocampal progenitor cells: A computational approach

Lee

Metz

McDiarmid

et al. 2021

Brain, Behavior, & Immunity - Health

View full text Add to dashboard Cite

show abstract

“…With regard to the mechanism, Ma et al demonstrated that there was a positive correlation between urinary Cu and plasma C-reactive protein (CRP) [ 28 ] and that CRP was associated with MetS [ 38 ]. Furthermore, our previous research showed that blood Cu concentration was positively associated with serum TNF-α [ 39 ]. We proposed that the association of Cu exposure with MetS may be on the basis of systemic inflammation.…”

Section: Discussionmentioning

confidence: 99%

The Relationship between Metabolic Syndrome and Plasma Metals Modified by EGFR and TNF-α Gene Polymorphisms

Chen

Kung²,

Sun

et al. 2021

Toxics

View full text Add to dashboard Cite

With the escalating global prevalence of metabolic syndrome (MetS), it is crucial to detect the high-risk population early and to prevent chronic diseases. Exposure to various metals has been indicated to promote MetS, but the findings were controversial, and the effect of genetic modification was not considered. Epidermal growth factor receptor (EGFR) was proposed to be involved in the pathway of metabolic disorders, and tumor necrotic factor-α (TNF-α) was regarded as an early inflammatory biomarker for MetS. This research aimed to analyze the impact of EGFR and TNF-α gene polymorphisms on the prevalence of MetS under environmental or occupational exposure to metals. We gathered data from 376 metal industrial workers and 639 non-metal workers, including physical parameters, biochemical data, and plasma concentrations of six metals. According to the genomic database of Taiwan Biobank, 23 single nucleotide polymorphisms (SNPs) on EGFR gene and 6 SNPs on TNF-α gene were incorporated in our research. We applied multivariable logistic regression to analyze the probability of MetS with various SNPs and metals. Our study revealed some susceptible and protective EGFR and TNF-α genotypes under excessive exposure to cobalt, zinc, selenium, and lead. Thus, we remind the high-risk population of taking measures to prevent MetS.

show abstract

“…In this context, metalworkers are exposed to a wide range of compounds, such as heavy metals, solvents, and metalworking fluids (MWF), that can negatively affect their health (Fornander et al, 2013;Hopf et al, 2019). Metals such as lead, cadmium, arsenic, and manganese are commonly related to metallurgy and are recognized as agents that can damage many target organs in the human body (Chen et al, 2019). In addition, a study conducted by Hamzah et al (2016) showed that cobalt and chromium levels were above the allowed exposure limit and that they were related to decreased lung function in workers exposed.…”

Section: Introductionmentioning

confidence: 99%

Accelerated apoptosis, oxidative stress, and cholinergic inflammation in blood of metalworkers

et al. 2021

View full text Add to dashboard Cite

Metalworkers are exposed to numerous chemicals in their workplace environment, such as solvents, heavy metals, and metalworking fluids, that have a negative impact on their health. Furthermore, there is an increase in the prevalence of chronic diseases among metalworkers; however, the molecular mechanisms involved in this increased predisposition to chronic diseases are unclear. Considering that occupational exposure represents a potential risk for metalworkers, the aim of this study was to measure biomarkers of oxidative stress, inflammation, and cytotoxicity in the peripheral blood of metalworkers from Southern Brazil. The study included 40 metalworkers and 20 individuals who did not perform activities with any recognized exposure to chemical substances, such as those working in administration, commerce, and education, as controls. Cellular and molecular biomarkers as leukocyte viability, intracellular production of reactive species, mitochondrial mass and membrane potential and plasma lipid peroxidation, sulfhydryl groups, total antioxidant capacity, and butyrylcholinesterase activity were evaluated in the blood of metalworkers and controls. Metalworkers were found to have higher rates of apoptosis, increased production of reactive species, and increased mitochondrial potential and mass in leukocytes associated with decreased antioxidant defenses and increased activity of the butyrylcholinesterase enzyme in their plasma. It can be concluded that cytotoxicity, oxidative stress, and inflammation are involved in the multiplicity of health outcomes related to chemical exposure in the metalworking industry.

show abstract

The Association of Serum TNF-α Levels and Blood Multi-Elements Modified by TNF-α Gene Polymorphisms in Metal Industrial Workers

Cited by 7 publications

References 40 publications

The effects of genotype on inflammatory response in hippocampal progenitor cells: A computational approach

The effects of genotype on inflammatory response in hippocampal progenitor cells: A computational approach

The Relationship between Metabolic Syndrome and Plasma Metals Modified by EGFR and TNF-α Gene Polymorphisms

Accelerated apoptosis, oxidative stress, and cholinergic inflammation in blood of metalworkers

Contact Info

Product

Resources

About