The association of speckle-type POZ protein with lymph node metastasis and prognosis in cancer patients

Cheng, Fei; Zeng, Chunyan; Wu, Chayan; Chen, Youxiang

doi:10.1097/md.0000000000017439

Cited by 4 publications

(2 citation statements)

References 32 publications

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“…26 A meta-analysis of nine studies including 928 patients with various cancers suggested that SPOP protein may be a useful prognostic biomarker in cancer patients. 27 We previously found that SPOP downregulation or S119N mutation resulted in loss of function in HB. We carried out various experiments to verify SPOP as an anti-oncogene and S119N as a loss-of-function mutation.…”

Section: Discussionmentioning

confidence: 97%

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S119N Mutation of the E3 Ubiquitin Ligase SPOP Suppresses SLC7A1 Degradation to Regulate Hepatoblastoma Progression

Zhang

Liu

et al. 2020

Molecular Therapy - Oncolytics

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A previous study on hepatoblastoma revealed novel mutations and cancer genes in the Wnt pathway and ubiquitin ligase complex, including the tumor suppressor speckle-type BTB/POZ (SPOP). Moreover, the SPOP gene affected cell growth, and its S119N mutation was identified as a loss-of-function mutation in hepatoblastoma. This study aimed to explore more functions and the potential mechanism of SPOP and its S119N mutation. The in vitro effects of SPOP on cell proliferation, invasion, apoptosis, and in vivo tumor growth were investigated by western blot analysis, Cell Counting Kit-8, colony formation assay, flow cytometry, and xenograft animal experiments. The substrate of SPOP was discovered by a protein quantification assay and quantitative ubiquitination modification assay. The present study further proved that SPOP functioned as an anti-oncogene through the phosphatidylinositol 3-kinase/Akt signaling pathway to affect various malignant biological behaviors of hepatoblastoma both in vitro and in vivo . Furthermore, experimental results also suggested that solute carrier family 7 member 1 (SLC7A1) might be a substrate of SPOP and influence cell phenotype by regulating arginine metabolism. In conclusion, these findings demonstrated the function of SPOP and revealed a potential substrate related to hepatoblastoma tumorigenesis, which might thus provide a novel therapeutic target for hepatoblastoma.

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Section: Discussionmentioning

confidence: 97%

“… 26 A meta-analysis of nine studies including 928 patients with various cancers suggested that SPOP protein may be a useful prognostic biomarker in cancer patients. 27 …”

Section: Discussionmentioning

confidence: 99%

S119N Mutation of the E3 Ubiquitin Ligase SPOP Suppresses SLC7A1 Degradation to Regulate Hepatoblastoma Progression

Zhang

Liu

et al. 2020

Molecular Therapy - Oncolytics

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Prevalence of SPOP and IDH Gene Mutations in Prostate Cancer in a Jordanian Population

Alorjani,

Al Bashir,

Al-Zaareer

et al. 2024

Biochem Genet

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Speckle-type POZ (SPOP) is described as an essential tumor suppressor factor in gastric cancer, colorectal cancer, and prostate cancer (PCa). SPOP gene mutations were reported in primary human PCa. Isocitrate dehydrogenase-1 (IDH1) oncogene mutations were detected in gliomas, acute myeloid leukemia, some benign and malignant cartilaginous tumors, and only 1% of PCa. This study aimed to investigate the prevalence of mutations of SPOP and IDH1 genes in PCa in the Jordanian population. One hundred formalin-fixed paraffin-embedded tissue samples were collected from patients diagnosed with prostate adenocarcinoma. The obtained specimens were subjected to genomic DNA extraction, PCR amplification, and direct sequencing of exons 4, 5, 6, and 7 of the SPOP gene and exon 6 of the IDH1 gene. SPOP gene mutations were found in 17% of PCa cases, while no mutation was detected in the screened exon 6 of the IDH1 gene. Clinicopathological data demonstrated a strong correlation between prostate-specific antigen (PSA) levels and both Gleason score (GS) and the International Society of Urological Pathology (ISUP) grade group (GG). There was no significant correlation between PSA levels and age (p = 0.816) nor there were significant associations for SPOP mutational status with age (p = 0.659), PSA levels (p = 0.395), GS (p = 0.259), and ISUP GG (p = 0.424) in the tested population. The study found a strong correlation between PSA levels and both GS and ISUP GG. It also identified a high frequency (17%) of SPOP gene mutations in Jordanian Arab PCa patients, mainly in exon 7. No IDH1 mutations were detected in exon 6.

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Clinicopathological and prognostic significance of speckle-type POZ protein in cancers: a systematic review and meta-analysis

Peng

Xia

et al. 2021

Cancer Cell Int

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Background Controversial findings have been reported in the impact of speckle-type POZ protein (SPOP) on clinicopathological features and prognosis in diverse cancers. We conducted this meta-analysis to confirm whether SPOP was an effective biomarker to predict clinical stage, cancer differentiation and survival. Methods We searched studies published before June 2021 through Medline, Embase, the Cochrane library register of controlled trials and Wanfang databases. The corrections of SPOP expression with expression disparity, tumor differentiation, clinical stage and survival were analyzed. Results Our meta-analysis found that higher expression of SPOP was significantly associated with earlier clinical stage, well differentiation and better overall survival. Subgroup analysis showed that the SPOP expression of adjacent tissue was significantly higher than that in cancer tissues of prostate and liver. However, renal cancer presented improved expression of SPOP in cancer tissue. Conclusions SPOP has the potential function to act as a novel and effective biomarker for cancer diagnosis and prognostic stratification.

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The association of speckle-type POZ protein with lymph node metastasis and prognosis in cancer patients

Abstract: Supplemental Digital Content is available in the text

Cited by 4 publications

References 32 publications

S119N Mutation of the E3 Ubiquitin Ligase SPOP Suppresses SLC7A1 Degradation to Regulate Hepatoblastoma Progression

S119N Mutation of the E3 Ubiquitin Ligase SPOP Suppresses SLC7A1 Degradation to Regulate Hepatoblastoma Progression

Prevalence of SPOP and IDH Gene Mutations in Prostate Cancer in a Jordanian Population

Clinicopathological and prognostic significance of speckle-type POZ protein in cancers: a systematic review and meta-analysis

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