The association of Th17/Treg cells expression in peripheral blood and chronic spontaneous urticaria

Yu, Qianying; Lin, Wenxia; Zhang, Jie; Peng, Li; Kong, Qiao-Qiao; Zhong, Rubin; Lan, Yan; Xiao, Min; Chen, Mingling

doi:10.1097/md.0000000000022014

Cited by 7 publications

(6 citation statements)

References 9 publications

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“…Conversely, Treg cells exhibited a significant negative correlation pruritus level, disease severity, and quality of life in CSU patients. These results suggest that the dysregulation of Th17/Treg in peripheral blood may contribute to the pathogenesis of CSU 38 . Similar associations between Th17/Treg cells and disease severity has been reported in other skin diseases, such as atopic dermatitis, where the ratio of Th17 cells is positively associated with disease severity, while the proportion of Treg cells is inversely correlated 39 .…”

Section: Discussionsupporting

confidence: 82%

“…These results suggest that the dysregulation of Th17/Treg in peripheral blood may contribute to the pathogenesis of CSU. 38 Similar associations between Th17/Treg cells and disease severity has been reported in other skin diseases, such as atopic dermatitis, where the ratio of Th17 cells is positively associated with disease severity, while the proportion of Treg cells is inversely correlated. 39 In psoriasis, the absence of Treg cells has been shown to contribute the disease maintenance and exacerbation.…”

Section: Discussionsupporting

confidence: 65%

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The correlation between Th17/Treg immune dysregulation and the disease severity in chronic spontaneous urticaria patients

Yang

Wang

et al. 2023

Immunity Inflam & Disease

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Objective Chronic spontaneous urticaria (CSU) has a profound impact on the sleep quality, productivity and overall quality of life of affected individuals. This study aimed to investigate the correlation between serum Th17/Treg immune dysregulation and the severity of CSU in patients. Methods Clinical baseline data of 120 CSU patients and matched healthy controls were recorded. The pruritus level, disease severity, and quality of life of CSU patients were assessed using the visual analogue scale, weekly Urticaria Activity Score and chronic urticaria quality of life questionnaire, respectively. The Th17/Treg cell ratio was detected by flow cytometry. ELISA was used to measure the levels of serum Th17 cytokines (IL‐17, IL‐21) and Treg cytokines (TGF‐β1, IL‐35). Pearson's correlation analysis was conducted to examine the associations between these indicators. Results No significant differences were identified in terms of sex, age, and BMI between the two groups. However, CSU patients exhibited a significant increase in the Th17 cell ratio, as well as the elevated serum levels of TGF‐β1, IL‐17 and, IL‐21. Conversely, the proportion of Treg cells and the levels of IL‐35 were remarkably decreased in CSU patients. Peripheral blood Th17 cells were negatively correlated with Treg cells. The severity of pruritus, life quality, and disease severity in CSU patients were positively correlated to Th17 cell ratio, and inversely correlated with Treg cell proportion. Conclusions A positive correlation was found between the percentage of peripheral blood Th17 cell in CSU patients and the pruritus level, life quality, and disease severity. In constrast, there was a negative correlation between the proportion of peripheral blood Treg cells and these clinical parameters.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionsupporting

confidence: 65%

The correlation between Th17/Treg immune dysregulation and the disease severity in chronic spontaneous urticaria patients

Yang

Wang

et al. 2023

Immunity Inflam & Disease

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“…We found decreased levels of mRNA for FOXP3 and IL10 genes. Earlier it was shown that circulating FOXP3+ Tregs are reduced and/or defective under CU [35]. Arshi and colleagues (2014) demonstrated a significant reduction of circulating CD4 + CD25 + FOXP3 + T cells in patients with CU compared to control individuals [36].…”

Section: Discussionmentioning

confidence: 99%

Analysis of the Transcriptional Activity of Immune Response Genes in the Blood of Patients with Acute Urticaria

Petruk

Kamyshna

Shkilna

et al. 2022

Open Access Maced J Med Sci

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OBJECTIVE: Alterations in the transcriptional activity of some immunoregulatory genes can play a key in the pathogenesis of acute urticarial (AU). Minimally-invasive markers of the transcriptional activity of immune response genes are essential not only for predicting the severity and activity of the disease but also as a potential target for therapy. METHODS: In our research, we applied a pathway-specific polymerase chain reaction PCR array (Human Innate and Adaptive Immune Responses RT2 Profiler PCR Array, QIAGEN, Germany) to detect and verify innate and adaptive immune responses pathway-focused genes expression in patients suffering from AU and control group. RESULTS: The AU development was accompanied by an increase in the transcriptional activity of genes for a number of costimulation molecules such as CD40, CD40LG, CD80 (B7-1), and C-reactive protein and myeloperoxidase genes either. Under AU conditions, transcriptional induction of genes of several cytokines was also observed: Interferon gamma, interleukin (IL4), IL5, IL17A, tumor necrosis factor, and also chemokine CXCL8. This process was also accompanied by an increase in the transcriptional activity of the RAR-related orphan receptor C Th17 differentiation regulator, the NLRP3 inflammasome genes, and the NFKB1 transcription factor. Such changes occurred against the background of transcriptional repression of the FOXP3 gene and the Treg-dependent suppressor cytokine IL10. The expression of other studied genes did not differ significantly from the controls. CONCLUSIONS: The development of acute urticaria led to the transcriptional activation of pro-inflammatory signaling against the background of a deficiency of the suppressor link. Detected changes in gene expression can be important for targeted therapy.

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“…Chen et al found that the Th1 and Th17 cytokines in plasma were significantly increased in CSU 24 . The meta‐analysis by Yu et al suggested that the imbalance of Th17/Treg cells in peripheral blood is involved in the pathogenesis of CSU 27 . These results imply that Th17 cells and IL‐17 may play a role in the pathogenesis of CSU, which, however, remains to be further studied due to limited evidence.…”

Section: Discussionmentioning

confidence: 99%

The hub genes and their potential regulatory mechanisms in chronic spontaneous urticaria revealed by integrated transcriptional expression analysis

Cao

et al. 2023

Experimental Dermatology

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Chronic spontaneous urticaria (CSU) is a recurrent disease characterized by wheals and or angioedema, and its pathogenesis is still unclear. The microarray datasets of skin tissue from CSU patients and healthy controls were integrated and analysed in Gene Expression Omnibus (GEO). Differentially expressed genes (DEGs) were identified using the NetworkAnalyst tool. Then, the Gene Ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed. Subsequently, a protein-protein interaction (PPI) network of DEGs was constructed by STRING and the related hub genes were identified through the MOCDE tool. The potential miRNAs targeting hub genes were predicted based on the intersection of three online databases, namely TargetScanHuman, TargetBase and miRNet. Differentially expressed lncRNAs (DElncRNAs) was performed using the GEO2R tool. The potential miRNAs targeting DElncRNAs were predicted through miRNet. Finally, the shared miRNAs targeting both hub genes and DElncRNAs were used to construct an mRNA/miRNA/lncRNA regulatory network. A total of 296 DEGs were obtained, which were mainly enriched in inflammatory and immune responses.Further, 14 hub genes were identified by the PPI network of DEGs. Clinical correlation analysis showed that the mRNA expressions of S100A7, S100A8, S100A9, S100A12, IL6 and SOCS3 in CSU were positively correlated with the 7-day urticaria activity score (UAS7), and their potential diagnostic value was supported by the receiver operating characteristic curve (ROC) analysis. Five up-regulated lncRNAs in the cytoplasm were obtained by DElncRNAs analysis. The ROC analysis showed that PVT1, SNHG3 and ZBTB20 − AS1 was of potential diagnostic value for CSU. Eight shared miRNAs targeting both hub genes and DElncRNAs were identified and used to construct a competing endogenous RNA (ceRNA) network. It was found that the IL-6/ miR − 149 − 5p/ZBTB20 − AS1 axis might play an important role in the activation of | 841 LIU et al.

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The association of Th17/Treg cells expression in peripheral blood and chronic spontaneous urticaria

Cited by 7 publications

References 9 publications

The correlation between Th17/Treg immune dysregulation and the disease severity in chronic spontaneous urticaria patients

The correlation between Th17/Treg immune dysregulation and the disease severity in chronic spontaneous urticaria patients

Analysis of the Transcriptional Activity of Immune Response Genes in the Blood of Patients with Acute Urticaria

The hub genes and their potential regulatory mechanisms in chronic spontaneous urticaria revealed by integrated transcriptional expression analysis

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