The Association Study of Targeted DNA Methylation and Thrombophilia

Abstract

Objective The aim of this study is to investigate the relationship of leukocytes DNA methylation in targeted sites and thrombophilia. Methods Eight thrombophilia patients and their kin-related individuals as the healthy control. Targeted DNA methylation from peripheral leukocytes were examined with MassArray. Multivariate correlation analysis was used to estimate targeted gene methylation as an independent risk factor of thrombophilia. Receiver operating characteristic curve analysis was used to calculate the accuracy of biomarkers in the prediction of thrombophilia. Results The age of thrombophilia group was higher than control group (P < 0.001). F5.24.CpG.10 and Protein S.44.CpG.29–33 methylation were significantly associated with thrombophilia negatively and positively (r = -0.7289, P < 0.01 and r = 0.5667, P < 0.05). F5.24.CpG.10 methylation was higher in control group (P < 0.01), but Protein S.44.CpG.29–33 methylation increased in thrombophilia group (P < 0.05). The areas under curve of ROC were 0.9297 and 0.8437, respectively. Conclusion Target DNA methylation in Protein S.44.CpG.29–33 island is associated with an elevated risk of thrombophilia.