Objective: This study aimed to verify the association between specific field-based physical fitness test results and selected health biomarkers in college-aged males. Method: A total of 390 males participated in this research. The association between fitness test scores and anthropometric and health variables were examined. The fitness tests conducted were: Sit-and-reach test (S&R), standing long jump test (SLJ), Shuttle run test (SHR), and 20 m Multistage Fitness Test (BT) to estimate the maximum oxygen uptake (VO2max). The anthropometric and health variables assessed were: Weight (WE), height (HE), body mass index (BMI), body fat percentage (FAT), lean body mass (LBM), abdominal Girth (AG), waist-to-hip ratio (WHR), systolic blood pressure (SBP), diastolic blood pressure (DBP), oxygen saturation (SPO2), average blood pressure (A-BP), double product (DP), and fasting blood glucose (GLU). Results: S&R presented a weak significant correlation with SLJ, VO2max, and AG. SLJ maintained weak to moderate significant correlations with S&R, SHR, VO2max, HE, WE, LBM, WHR, BMI, FAT, AG, SBP, DBP, A-BP, DP, and GLU. SHR presented weak to moderate significant correlations with SLJ, VO2max, WE, BMI, AG, FAT, HE, SBP, DP, and GLU. VO2max maintained weak to moderate correlations with S&R, SLJ, SHR, WE, BMI, FAT, LBM, AG, and DP. Weak to moderate correlations were found between anthropometric and health variables, whereas the anthropometric variables presented significant correlations with each other, ranging from weak to very strong. Fitness test results presented weak to moderate correlations among themselves. Conclusion: SLJ and SHR present weak to moderate validity to predict the selected anthropometric markers and weak to predict the selected health indicators except for SPO2. VO2max has only weak validity to predict the selected anthropometric markers, whereas S&R is not valid to predict the selected health or anthropometric markers. Anthropometric measurements have weak validity in predicting the selected health markers. BMI and AG are valid, simple, and economical measurements to assess body fat. A positive interaction between the results obtained in the field-based fitness tests conducted was observed.