The ATP/ADP Antiporter is Involved in the Uncoupling Effect of Fatty Acids

Andreyev, Alexander Y.; Bondareva, T. O.; Dedukhova, V.I.; Mokhova, E. N.; Skulachev, Vladimir P.; Tsofina, L. M.; Volkov, N. I.; Vygodina, Tatiana V.

doi:10.1007/978-3-642-74539-3_13

Cited by 6 publications

(5 citation statements)

References 10 publications

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“…While birds do not possess the heat-producing brown adipose tissue of mammals (Johnston 1971;Olson et al 1988;Saarela et al 1991), avian analogues of the previously mentioned regulatory factors, such as avian uncoupling protein (avUCP), do exist. Uncoupling proteins cause significant inhibitor-sensitive (nucleotide-sensitive) inducible proton conductance when activated by small molecules such as fatty acids, alkenals (Andreyev et al 1989;Brand 2000;Esteves and Brand 2005;Echtay et al 2003;Skulachev 1998), or AMP (Cadenas et al 2000). Though the precise role of avUCP is not well-known, mammalian homologues UCP2 and UCP3 are thought to play a role in regulation of lipid as fuel substrates, control of insulin secretion, and/or in the control of reactive oxygen species (ROS) production .…”

Section: Developmental Patterns Of Gene Expressionmentioning

confidence: 99%

The 12-day thermoregulatory metamorphosis of Red-winged Blackbirds (Agelaius phoeniceus)

Sirsat

Crossley

et al. 2016

J Comp Physiol B

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We examined development of endothermy in altricial Red-winged Blackbirds (Agelaius phoeniceus) by measuring oxygen consumption [Formula: see text], body temperature and ventilation at ambient temperatures from 35 to 15 °C. Mitochondrial respiration of permeabilized skeletal muscle was also measured from breast (pectoralis) and thigh (femorotibialis) muscles. Animals were studied from the first day of hatching through fledging (12 days post-hatch, dph). Nestling whole-body metabolic rate began to show an endothermic response to cold temperature midway between hatching and fledging. Nestlings less than 5 dph were unable to maintain elevated [Formula: see text] and body temperature when exposed to gradually decreasing temperature, whereas 7 dph nestlings maintained [Formula: see text] until ~25 °C, after which [Formula: see text] decreased. From 10 dph to fledging, animals maintained elevated [Formula: see text] and body temperature when exposed to gradual cooling; full endothermic capacity was achieved. Ventilation followed a similar developmental trend to that of [Formula: see text], with increases in 10 dph fledglings occurring in tidal volume rather than ventilation frequency. LEAK respiration and oxidative phosphorylation (OXPHOS) through complex I of breast muscle mitochondria increased significantly after 3 dph. Expression of avUCP and PCG-1α mRNA increased significantly at 3 dph and remained elevated in both skeletal muscle types. Increased metabolic capacity at the cellular level occurred prior to that of the whole animal. This change in whole animal metabolic capacity increased steadily upon hatching as evidenced by the shift of metabolic rate from an ectothermic to endothermic phenotype and the increase of mitochondrial OXPHOS activity of the shivering muscles of this altricial avian species.

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Section: Developmental Patterns Of Gene Expressionmentioning

confidence: 99%

The 12-day thermoregulatory metamorphosis of Red-winged Blackbirds (Agelaius phoeniceus)

Sirsat

Crossley

et al. 2016

J Comp Physiol B

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“…6. Fatty-acid proton carriers have been proposed in brown adipose tissue, using the uncoupler protein 'thermogenin' to flip and return dissociated fatty-acid anions after proton release [13]. Media were inoculated at 1 mg dry weight/l.…”

Section: Discussionmentioning

confidence: 99%

“…This may be either by dissociation in the cytoplasm or as a membrane proton carrier. Fatty-acid proton carriers have been proposed in brown adipose tissue, using the uncoupler protein 'thermogenin' to flip and return dissociated fatty-acid anions after proton release [13]. No such protein has yet been reported in yeast, thus both mechanisms remain possible.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the inhibitory action on Saccharomyces cerevisiae of weak-acid preservatives, uncouplers, and medium-chain fatty acids

Stratford

1996

FEMS Microbiology Letters

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This study was initiated to establish whether inhibition of growth of yeasts by medium-chain fatty acids resembled that caused by weak-acid preservatives or uncouplers. Unlike sorbic acid and 2,4-dinitrophenol, decanoic acid caused rapid cell death at its inhibitory concentration. This suggested a mode of action by medium-chain fatty acids, distinct from both weak-acid preservatives and uncouplers. Sorbic acid and 2,4-dinitrophenol both increased lag and doubling times, reduced cell yields and inhibitory concentrations of both were highly pH sensitive. The possibility is discussed as to whether weak-acid preservatives and uncouplers share common modes of inhibition.

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“…The FFA-dependent H + re-cycling is a side function of these carriers and can be inhib-ited by their translocated substrates or specific inhibitors. Thus, the FFA-induced uncoupling could be mediated, at least in part, by the ATP/ADP antiporter [28] [18,20]. Addition of FFA to isolated respiring mitochondria results in mitochondrial uncoupling revealed by an increase in resting (state 4) respiration, a decrease in membrane potential and a decrease in the ADP/O ratio (Fig.…”

Section: Mitochondrial Uncoupling By Other Carriersmentioning

confidence: 96%

Uncoupling proteins in mitochondria of plants and some microorganisms.

Jarmuszkiewicz¹

2001

Acta Biochim Pol

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Uncoupling proteins, members of the mitochondrial carrier family, are present in mitochondrial inner membrane and mediate free fatty acid-activated, purine-nucleotide-inhibited H+ re-uptake. Since 1995, it has been shown that the uncoupling protein is present in many higher plants and some microorganisms like non-photosynthetic amoeboid protozoon, Acanthamoeba castellanii and non-fermentative yeast Candida parapsilosis. In mitochondria of these organisms, uncoupling protein activity is revealed not only by stimulation of state 4 respiration by free fatty acids accompanied by decrease in membrane potential (these effects being partially released by ATP and GTP) but mainly by lowering ADP/O ratio during state 3 respiration. Plant and microorganism uncoupling proteins are able to divert very efficiently energy from oxidative phosphorylation, competing for deltamicroH+ with ATP synthase. Functional connection and physiological role of uncoupling protein and alternative oxidase, two main energy-dissipating systems in plant-type mitochondria, are discussed.

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The ATP/ADP Antiporter is Involved in the Uncoupling Effect of Fatty Acids

Cited by 6 publications

References 10 publications

The 12-day thermoregulatory metamorphosis of Red-winged Blackbirds (Agelaius phoeniceus)

The 12-day thermoregulatory metamorphosis of Red-winged Blackbirds (Agelaius phoeniceus)

Comparison of the inhibitory action on Saccharomyces cerevisiae of weak-acid preservatives, uncouplers, and medium-chain fatty acids

Uncoupling proteins in mitochondria of plants and some microorganisms.

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