Biomotors and Their Nanobiotechnology Applications 2023
DOI: 10.1201/9780429203367-14
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The ATPase of the phi29 DNA Packaging Motor Is a Member of the Hexameric AAA+ Superfamily

Abstract: The AAA+ superfamily of proteins is a class of motor ATPases performing a wide range of functions that typically exist as hexamers. The ATPase of phi29 DNA packaging motor has long been a subject of debate in terms of stoichiometry and mechanism of action. Here, we confirmed the stoichiometry of phi29 motor ATPase to be a hexamer and provide data suggesting that the phi29 motor ATPase is a member of the classical hexameric AAA+ superfamily. Native PAGE, EMSA, capillary electrophoresis, ATP titration, and binom… Show more

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