The Augmenting Effects of the tDNA Insulator on Stable Expression of Monoclonal Antibody in Chinese Hamster Ovary Cells

Naderi, Fatemeh; Hashemi, Mehrdad; Bayat, Hadi; Mohammadian, Omid; Pourmaleki, Es’hagh; Etemadzadeh, Mohammad Hossein; Rahimpour, Azam

doi:10.1089/mab.2018.0015

Cited by 6 publications

(1 citation statement)

References 32 publications

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“…Therefore, selecting an appropriate promoter and optimizing the combination of promoters and different regulatory elements can improve the expression of recombinant antibodies and increase the stability of expression. Some chromatin-modifying elements can prevent transgene silencing, including matrix attachment regions (MAR) ( Buceta et al, 2011 ; Mohammadian et al, 2019 ; Zhang et al, 2020 ), locus control regions (LCR) ( Sharma et al, 2019 ; Morgan et al, 2020 ), ubiquitous chromatin opening elements (UCOEs) ( Pfaff et al, 2013 ; Harraghy et al, 2015 ; Nematpour et al, 2017 ; Rocha-Pizaña et al, 2017 ), stabilizing anti-repressor elements (STAR) ( Kwaks et al, 2003 ; Otte et al, 2007 ; Van Blokland et al, 2007 ), and insulators ( Benabdellah et al, 2014 ; Chetverina et al, 2014 ; Naderi et al, 2018 ; Pérez-González and Caro, 2019 ). Moreover, artificial chromosome expression (ACE) and targeted integration technology, including Flp-In and recombinase-mediated cassette exchange system (RMCE), can overcome the shortcomings of random integration ( Kennard et al, 2009 ; Soler et al, 2018 ; Reinhart et al, 2019 ; Ng et al, 2021 ).…”

Section: Construction and Optimization Of Expression Vectorsmentioning

confidence: 99%

Strategies and Considerations for Improving Recombinant Antibody Production and Quality in Chinese Hamster Ovary Cells

Zhang

Shan

Liang

et al. 2022

Front. Bioeng. Biotechnol.

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Recombinant antibodies are rapidly developing therapeutic agents; approximately 40 novel antibody molecules enter clinical trials each year, most of which are produced from Chinese hamster ovary (CHO) cells. However, one of the major bottlenecks restricting the development of antibody drugs is how to perform high-level expression and production of recombinant antibodies. The high-efficiency expression and quality of recombinant antibodies in CHO cells is determined by multiple factors. This review provides a comprehensive overview of several state-of-the-art approaches, such as optimization of gene sequence of antibody, construction and optimization of high-efficiency expression vector, using antibody expression system, transformation of host cell lines, and glycosylation modification. Finally, the authors discuss the potential of large-scale production of recombinant antibodies and development of culture processes for biopharmaceutical manufacturing in the future.

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