The autolysin of Porphyromonas gingivalis is involved in outer membrane vesicle release

Hayashi, Jun-ichiro

doi:10.1016/s0378-1097(02)01041-8

Cited by 17 publications

(20 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Autolysins are murein hydrolases that cleave covalent bonds in the peptidoglycan and they are responsible for the cell wall remodeling, peptidoglycan turnover, cell division and peptidoglycan cell wall reparation (Shockman, 1994). In agreement with their role in cell wall remodeling, this autolysin mutant strain exhibits impaired cell division (Hayashi et al, 2002). The imbalance between cell wall turnover and OM biosynthesis could be responsible for the increased OMV secretion (Hayashi et al, 2002).…”

Section: Peptidoglycan Fragments Accumulationmentioning

confidence: 89%

“…The accumulation of the peptidoglycan fragments in the periplasmic space could therefore exert a turgor pressure strong enough to bend the OM and produce OMVs (Figure 2A) (Zhou et al, 1998). An increase in OMV production was observed when peptidoglycan fragments accumulated because of the incomplete degradation of the peptidoglycan in a Porphyromonas gingivalis autolysin mutant strain (Hayashi et al, 2002). Autolysins are murein hydrolases that cleave covalent bonds in the peptidoglycan and they are responsible for the cell wall remodeling, peptidoglycan turnover, cell division and peptidoglycan cell wall reparation (Shockman, 1994).…”

Section: Peptidoglycan Fragments Accumulationmentioning

confidence: 99%

See 1 more Smart Citation

Prokaryotic membrane vesicles: new insights on biogenesis and biological roles

Haurat¹,

Elhenawy

Feldman

2014

Biological Chemistry

125

123

View full text Add to dashboard Cite

Biogenesis and trafficking of membrane vesicles are essential and well-studied processes in eukaryotes. In contrast, vesiculation in bacteria is not well understood. Outer membrane vesicles (OMVs) are produced in Gramnegative bacteria by blebbing of the outer membrane. In addition to the roles in pathogenesis, cell-to-cell communication and stress response, recent work has suggested that OMVs play important roles in immunomodulation and the establishment and balance of the gut microbiota. In this review we discuss the known and novel roles of OMVs and the different biogenesis models proposed, and address the evidence for cargo selection into OMVs. We also discuss the growing evidence for the existence of membrane vesicles in Gram-positive bacteria and Archaea. Due to their biological importance and promising applications in vaccinology, the biogenesis of OMVs is an important topic in microbiology.

show abstract

Section: Peptidoglycan Fragments Accumulationmentioning

confidence: 89%

Section: Peptidoglycan Fragments Accumulationmentioning

confidence: 99%

Prokaryotic membrane vesicles: new insights on biogenesis and biological roles

Haurat¹,

Elhenawy

Feldman

2014

Biological Chemistry

125

123

View full text Add to dashboard Cite

show abstract

“…Surface blebbing describes the extrusion of the outer membrane that leads to shedding of MVs, which are prominent in many Gram-negative bacteria and may contain virulence factors such as proteases, toxins, LPS and even DNA (Grenier & Mayrand 1987, Kadurugamuwa & Beveridge 1995, Beveridge 1999, Keenan et al 2000, Yaron et al 2000, Hayashi et al 2002. The percentage of blebbing F. psychrophilum cells was generally very high, but for strain NCMB1947 a marked increase was observed in cultures grown under iron limitation produced by supplementing the medium with dipyridyl or serum, indicating that certain stress factors may increase surface blebbing and MVs release.…”

Section: Discussionmentioning

confidence: 99%

Characterisation of surface blebbing and membrane vesicles produced by Flavobacterium psychrophilum

Møller¹,

Barnes²,

Dalsgaard³

et al. 2005

Dis. Aquat. Org.

View full text Add to dashboard Cite

The surface of Flavobacterium psychrophilum was examined by electron microscopy to determine if previous findings of haemagglutination positive (HA+) and haemagglutination negative (HA-) abilities could be correlated with expression of pili or of a capsular layer. A thin capsular layer was observed in both HA+ and HA-strains but typical pili were absent. However, long, tubular blebs that released membrane vesicles (MVs) into the supernatant were observed on up to 94% of cells within 1 sample. The surface blebbing was increased for 1 strain following growth on media with restricted iron availability. The MVs had an intact membrane bilayer and were released from blebbing cells of both strains. The protein profiles of MVs, while containing some banding similarity with the profile of outer membrane preparations (OMPs) and of lysed whole cells (WCs), showed several bands that reacted strongly with rabbit anti-whole-cell antisera. Two distinct bands of approximately 62 and 58 kDA were highly expressed in the MVs and not seen in the OMP. MVs contained proteolytic activity towards gelatine but not towards casein and elastin, which were only degraded by live cells. Low molecular weight lipopolysaccharides (LPS) or lipooligosaccharides (LOS) were associated with the MVs. Only the MVs of the HA+ strain possessed haemagglutinin activity. These findings suggest that the F. psychrophilum may, through surface blebbing, release antigenic MVs that contain some proteolytic activity and may aid the bacterium in releasing nutrients from its surrounding environment as well as playing a role in impeding the immune response of its host. KEY WORDS: Flavobacterium psychrophilum · Membrane vesicles · Surface blebbing · Electron microscopy Resale or republication not permitted without written consent of the publisherDis Aquat Org 64: [201][202][203][204][205][206][207][208][209] 2005 tion positive (HA+) strains and absent on HA-strains (Møller et al. 2003). Lectins and haemagglutinins are often expressed on or in connection with the tip of the long, threadlike surface structures known as pili. Flavobacterium (formerly known as Cytophaga as well as Flexibacter) is generally considered to lack pili expression (Henrichsen & Blom 1975, Pate 1985, McBride 2001 and F. psychrophilum was previously reported to lack pili (Holt et al. 1993, Lorenzen et al. 1997. One member of this group of bacteria, the fish pathogenic agent of bacterial gill disease, Flavobacterium branchiophila (now F. branchiophilum) is, however, reported to possess pili (Heo et al. 1990). A thin, more or less compact slime or capsular layer surrounds F. psychrophilum and may aid in adhesion (Crump et al. 2001). The present study was initiated to determine, by electron microscopy, whether the haemagglutinating ability present in some F. psychrophilum strains was correlated with pili or capsular expression.Several pathogenic Gram-negative bacteria including Escherichia coli O157:H7 (Kolling & Matthews 1999), Pseudomonas aeruginosa (Kadurugamuwa & Beveridge 1995), Nei...

show abstract

“…Zhou et al (1998) studied the biogenesis of OMVs from Porphyromonas gingivalis and proposed a model that suggested that OMV production was due to turnover of the cell wall. This model was further revisited by showing that autolysin mutants of Porphyromonas gingivalis produced fewer OMVs than wild-type strains (Hayashi et al, 2002). During cell wall synthesis, peptidoglycan and muramic acid exert a turgor pressure onto the OM.…”

Section: Omvs As a Product Of Cell Wall Turnovermentioning

confidence: 99%

Biogenesis and multifaceted roles of outer membrane vesicles from Gram-negative bacteria

2014

View full text Add to dashboard Cite

Outer membrane vesicles (OMVs) released from Gram-negative bacteria consist of lipids, proteins, lipopolysaccharides and other molecules. OMVs are associated with several biological functions such as horizontal gene transfer, intracellular and intercellular communication, transfer of contents to host cells, and eliciting an immune response in host cells. Although hypotheses have been made concerning the mechanism of biogenesis of these vesicles, research on OMV formation is far from complete. The roles of outer membrane components, bacterial quorum sensing molecules and some specific proteins in OMV biogenesis have been studied. This review discusses the different models that have been proposed for OMV biogenesis, along with details of the biological functions of OMVs and the likely scope of future research.

show abstract

The autolysin of Porphyromonas gingivalis is involved in outer membrane vesicle release

Cited by 17 publications

References 25 publications

Prokaryotic membrane vesicles: new insights on biogenesis and biological roles

Prokaryotic membrane vesicles: new insights on biogenesis and biological roles

Characterisation of surface blebbing and membrane vesicles produced by Flavobacterium psychrophilum

Biogenesis and multifaceted roles of outer membrane vesicles from Gram-negative bacteria

Contact Info

Product

Resources

About