2013
DOI: 10.1016/j.mrgentox.2012.10.011
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The automated micronucleus assay for early assessment of genotoxicity in drug discovery

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Cited by 21 publications
(12 citation statements)
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“…). In agreement, publications by Diaz et al () and Tilmant et al () reported that the CBPI was less meaningful than the RCC and therefore they used the cell number to determine cytotoxicity. The apparent discrepancy between these cytotoxicity parameters is still unclear.…”
Section: Discussionsupporting
confidence: 74%
“…). In agreement, publications by Diaz et al () and Tilmant et al () reported that the CBPI was less meaningful than the RCC and therefore they used the cell number to determine cytotoxicity. The apparent discrepancy between these cytotoxicity parameters is still unclear.…”
Section: Discussionsupporting
confidence: 74%
“…In order to cover a broad test concentration range in our set up, we tested compounds in a ten point dose response, in duplicate, which allows for four compounds to be tested per plate. Throughput was our main reason to focus on the flow cytometric MN scoring method, though there are other automated methods that can be used to screen for MN formation, such as high content analysis (HCA) which has been investigated using the adherent hamster cell line CHO‐K1 [Mondal et al, ; Westerink et al, ; Tilmant et al, ]. The use of suspension cell lines, such as TK6, has not been described for the HCA method, whereas the flow cytometric method can be used for both adherent and suspension cell cultures [Bryce et al, ].…”
Section: Discussionmentioning
confidence: 99%
“…Since actual carcinogenicity can only be assessed in lengthy studies in animals, which are often performed later during development, it is essential to identify and deselect genotoxic compounds in early stages of drug discovery. The in vitro micronucleus assay has emerged as a widely accepted tool for genotoxicity testing, due to its high sensitivity and specificity, and ability to detect both aneugenic and clastogenic agents [23,24]. Micronuclei are either entire chromosomes that have failed to be incorporated properly in daughter nuclei during cell division, which is known as an aneugenic event, or they are pieces of damaged chromosomes resulting from double-strand breaks, which is known as a clastogenic event.…”
Section: Genotoxicitymentioning
confidence: 99%