The autotrophic growth of <i>Micrococcus denitrificans</i> on Methanol

Cox, R. B.; Quayle, J. R.

doi:10.1042/bj1500569

Cited by 102 publications

(51 citation statements)

References 18 publications

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“…In future work with Thiobacillus A2, its reported ability (Taylor & Hoare, 1969) to grow on methanol needs reinvestigation : if it proves capable of growth on methanol, it will be of considerable importance to determine if carbon assimilation from methanol as well as formate proceeds by the Calvin cycle as in Paracoccus denitrzjicans (Cox & Quayle, 1975;van Verseveld & Stouthamer, 1978), or whether it is able to develop the ribulose monophosphate or serine pathway for methanol metabolism as in the methylotrophs (Whitten-…”

Section: Discussionmentioning

confidence: 99%

Autotrophic Metabolism of Formate by Thiobacillus Strain A2

Kelly

Wood

Gottschal

et al. 1979

Journal of General Microbiology

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Thiobacillus A2 grew on formate as its sole source of carbon and energy. Growth was autotrophic : formate and carbon dioxide were equivalent as carbon sources during growth on formate. Carbon dioxide was fixed by the Calvin cycle in formate-or thiosulphate-grown bacteria which contained comparable high specific activities of ribulosebisphosphate carboxylase. Formate incorporated by bacteria growing heterotrophically or on thiosulphate showed more restricted metabolism, particularly providing carbon for purines. Bacteria growing on formate or thiosulphate assimilated acetate but showed disproportionately high incorporation into glutamate, proline, arginine, leucine, pyrimidines and lipid. Growth kinetics on formate were studied using extended cultures held at constant formate concentrations at pH 7-8. Yield was a function of substrate concentration and growth rate, which were linearly related in the range 4 to 40 mmformate. Formate was an inhibitory substrate at higher concentrations. A computer analysis of the inhibited growth kinetics indicated K, and values of 26.5 and 187 mmformate, respectively, and a true pmar of 0.299 he1.

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Section: Discussionmentioning

confidence: 99%

Autotrophic Metabolism of Formate by Thiobacillus Strain A2

Kelly

Wood

Gottschal

et al. 1979

Journal of General Microbiology

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“…In the present paper the important theoretical studies of van Dijken & Harder (1975) have been modified and extended in response to the many recent developments in our knowledge of the biochemistry of methylotrophs which include elucidation of a variety of carbon assimilation pathways (Strarm, Ferenci & Quayle, 1974;Colby & Zatman, 1975;Cox & Quayle, 1975;Anthony, 1975a), the understanding of the two different methane hydroxylase systems (Tonge, Harrison & Higgins, I977b;Colby, Dalton & Whittenbury, 1975;Colby & Dalton, 1976;Colby, Stirling & Dalton, 1977) and the relatively low P/O ratios associated with NADH oxidation indicated in some methylotrophs (Tonge, Drozd & Higgins, 1977a;O'Keeffe & Anthony, 1978;Anthony & O'Keeffe, 1977).…”

Section: Introductionmentioning

confidence: 99%

The Prediction of Growth Yields in Methylotrophs

Anthony

1978

Journal of General Microbiology

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Equations have been developed for assimilation of methane, methanol, methylamine, dimethylamine, trimethylamine, formaldehyde and formate into cell material by bacteria using the ribulose monophosphate pathway, the ribulose bisphosphate pathway and the serine pathway of carbon assimilation. The equations have been used for predicting the effect on cell yields (Y,, Yo2 and Yc0J of various P/O ratios, systems for substrate oxidation and assimilation pathways. The generalizations relating P/O ratios and cell yields, which have been used previously for such calculations, are not always applicable to methylotrophs. In particular, for the majority of methylotrophs the growth yield is determined by NAD(P)H supply as well as ATP supply, and for some methylotrophs growth yields may be exclusively NAD(P)H-limited. Because of this NAD(P)H limitation, the concept of YATP in methylotrophs should be used with extreme caution.

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“…denirr(ficans was supplemented with 0.01 % (w/v) yeast extract (Cox & Quayle, 1975). For autotrophic growth on methanol, formate or H , / C 0 2 , the medium was further supplemented with 0.02% (w/v) K H C 0 3 .…”

mentioning

confidence: 99%

Methanol Dissimilation in Xanthobacter H4-14: Activities, Induction and Comparison to Pseudomonas AMI and Paracoccus denitrificans

Weaver

Lidstrom

1985

Microbiology

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Methanol dissimilatory enzymes detected in the methanol autotroph Xanthobacter H4-14 were a typical phenazine methosulphate-linked methanol dehydrogenase, a NAD+-linked formate dehydrogenase, and a dye-linked formaldehyde dehydrogenase that could be assayed only by activity stains of polyacrylamide gels. This same methanol dehydrogenase activity was found in ethanol-grown cells and was apparently utilized for ethanol oxidation. Formaldehyde dehydrogenase activities were investigated in Paracoccus denitrficans, Xanthobacter H4-14, and Pseudomonas AM 1. P. denitrificans contained a previously reported NAD+-linked, GSHdependent activity, but both Xanthobacter H4-14 and Pseudomonus AM 1 contained numerous activities detected by activity stains of polyacrylamide gels. Induction studies showed that in Xanthobacter H4-14, a 10 kDal polypeptide, probably a dehydrogenase-associated cytochrome L', was co-induced with methanol dehydrogenase, but the formaldehyde and formate dehydrogenases were not co-regulated. Analogous induction experiments revealed similar patterns in P. denitrificans, but no evidence for co-regulation of dissimilatory activities in Pseudomonus AM 1.

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The autotrophic growth of Micrococcus denitrificans on Methanol

Abstract: Ribulose bisphosphate carboxylase is present at a high specific activity in extracts of methanol-grown Microccus denitrificans. Enzymic and physiological evidence indicates that, during growth on methanol, the ribulose bisphosphate cycle is the route of carbon assimilation.

Cited by 102 publications

References 18 publications

Autotrophic Metabolism of Formate by Thiobacillus Strain A2

Autotrophic Metabolism of Formate by Thiobacillus Strain A2

The Prediction of Growth Yields in Methylotrophs

Methanol Dissimilation in Xanthobacter H4-14: Activities, Induction and Comparison to Pseudomonas AMI and Paracoccus denitrificans

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