The bacterial communication system and its interference as an antivirulence strategy

Aggarwal, Suruchi; Mahajan, Pranav; Gupta, Puneet; Yadav, Alka; Dhawan, Gagan; Dhawan, Uma; Yadav, Amit Kumar Singh

doi:10.1016/b978-0-323-91806-0.00010-2

Cited by 2 publications

(2 citation statements)

References 57 publications

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“…[41]. However, S. aureus is also one of the top causes of hospital-and community-acquired infections globally due to its ability to grow in the circulation and in numerous tissues, causing significant disease [42,43]. It can trigger anything from a simple skin infection to more serious systemic infections that can even be fatal, such pneumonia, osteomyelitis, and endocarditis.…”

Section: Discussionmentioning

confidence: 99%

Studying Anti-virulence Activity of Meta-Bromo-Thiolactone against <em>Staphylococcus aureus</em> and MRSA Phenotypes

Alfaraj,

Eltayb,

AlFayez

et al. 2023

Preprint

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Of late, the focus has been shifted towards quorum sensing inhibitors which reduce bacterial virulence and lower the probability of resistance and refining infections. In this work, meta-bromo-thiolactone (mBTL), a potent quorum and virulence inhibitor against Staphylococcus aureus and MRSA phenotypes, was formulated in chitosan nanoparticles (ChNPs) using ionic gelation method. mBTL-loaded-ChNPs were characterized for particle size, polydispersity index, zeta potential. Morphology was visualized by Transmission Electron Microscopy (TEM), drug release profile and antibiofilm analysis using Confocal Laser Scanning Microscope (CLSM) and Scanning Electron Microscopy (SEM) were performed. Synthesized mBTL-loaded-CNPs showed homogenized nano-size particles ranging from 158+1.3 to 284+5.6 nm with spherical particles that exhibited sustainable release profile over 48 hr at 37 °C. These findings revealed successful preparation of mBTL-loaded-ChNPs, that further showed effective antibiofilm activity at MIC50 (0.5 mg/mL) where all strains displayed reduced biofilm formation compared to untreated strains. CLSM results showed a significant reduction in the number of viable cells, indicating the effectiveness of m-BTL as an antibacterial agent. SEM analysis permitted visualization of biofilm structure in relation to the spatial localization of important biofilm matrix components, the formed biofilms were clearly distinguished in the SEM images. Bacterial cells in the control group were enclosed in thick biofilms. In contrast, there was a considerable reduction in biofilm production when mBTL was present, where bacterial cells seemed less ordered and more scattered with no detectable biofilms. In conclusion, mBTL-loaded-ChNPs is a potential alternative treatment to overcome antimicrobial resistance and condensed MRSA infections.

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Section: Discussionmentioning

confidence: 99%

Studying Anti-virulence Activity of Meta-Bromo-Thiolactone against <em>Staphylococcus aureus</em> and MRSA Phenotypes

Alfaraj,

Eltayb,

AlFayez

et al. 2023

Preprint

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“…Pembuatan patch transdermal ekstrak etanol jahe emprit menggunakan tipe matriks, dimana zat aktif dilarutkan bersama dengan polimer, sehingga dapat menghindari lonjakan pelepasan zat aktif atau dumping dosage 24 . HPMC merupakan polimer hidrofilik yang ditujukan meningkatkan permebilitas matriks sehingga difusi obat melalui matriks lebih cepat dibandingkan dengan polimer hidrofobik dan erpengaruh dalam menentukan dan mengontrol kecepatan pelepasan obat 25 .…”

Section: Pembahasanunclassified

Formulation And Effectiveness Test of Tansdermal Patch of Ethanol Extract of Ginger (Zingiber officinale Rosc. Var Amarum) As Antiemetic

Syarifah,

L. Charisma,

Nabila

et al. 2023

IJPST

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Shogaol and gingerol, compounds in ginger, can act as antiemetic. Ginger extract was formulatedby patch transdermal to increase the effectiveness of therapy and to minimize the side effects. Inthis study, maceration with ethanol 96% was used as extraction method and phytochemical screeningwas carried out to the extract to identfy the exctracted compounds. The formulation of transdermalpatch used matrix method with ginger extract concentrations of 0,5 gr (F1), 1 gr (F2), and 1,5 gr (F3).Evaluation of patch was carried out by organoleptic test, patch weight, thickness, tensile strength,elongation at break, modulus young, moisturizer test, and folding endurance. Effectiveness test usedwistar mice by observed the pica response and a decreasing in kaolin consumption. The yield of theextract obtained was 6,46% and it contained essential oils, flavonoids, steroids, and polyphenols. Thebest patch characteristics of the concentration was F3 with a patch weight of 0,39 gr, thickness 0,23mm, tensile strength 1,32 N/mm2, elongation at break 129,52%, young's modulus 0,96 mPa, humidity1,32%, and folding endurance >200 times. F3 was also the most effective formulation with 6%response of pica and 93,11% of decreasing in kaolin consumption observed in day 9. In conclusion, F3can reduce nausea and vomiting.

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The bacterial communication system and its interference as an antivirulence strategy

Cited by 2 publications

References 57 publications

Studying Anti-virulence Activity of Meta-Bromo-Thiolactone against <em>Staphylococcus aureus</em> and MRSA Phenotypes

Studying Anti-virulence Activity of Meta-Bromo-Thiolactone against <em>Staphylococcus aureus</em> and MRSA Phenotypes

Formulation And Effectiveness Test of Tansdermal Patch of Ethanol Extract of Ginger (Zingiber officinale Rosc. Var Amarum) As Antiemetic

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