The bacterial flora of the midgut of two Danish populations of healthy fifth instar larvae of the turnip moth, Scotia segetum

Charpentier, R.; Charpentier, Bernadette; Zethner, O.

doi:10.1016/0022-2011(78)90174-x

Cited by 11 publications

(4 citation statements)

References 8 publications

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“…Indications of this microbial diversity were already obtained in early studies by Steinhaus, in which 83 different bacteria were isolated by cultivation from the digestive tracts of 30 insect species (Steinhaus, 1941). Since then, many more bacteria have been characterized from the guts of insects (see, for examples, Charpentier et al. , 1978; Ulrich et al.…”

Section: Introductionmentioning

confidence: 99%

Diversity and phylotype consistency of bacteria in the guts of three bee species (Apoidea) at an oilseed rape field

Mohr

Tebbe

2005

Environmental Microbiology

240

239

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The gut of insects may harbour one of the largest reservoirs of a yet unexplored microbial diversity. To understand how specific insects select for their own bacterial communities, the structural diversity and variability of bacteria found in the gut of different bee species was analysed. For three successive years, adults and larvae of Apis mellifera ssp. carnica (honey bee), and Bombus terrestris (bumble bee), as well as larvae of Osmia bicornis (red mason bee) were collected at a flowering oilseed rape field. Total DNA was extracted from gut material and the bacterial diversity was analysed, independent of cultivation, by genetic profiling with single-strand conformation polymorphism (SSCP) of polymerase chain reaction (PCR)-amplified partial 16S rRNA genes. The SSCP profiles were specific for all bee species and for larvae and adults. Qualitative and quantitative differences were found in the bacterial community structure of larvae and adults of A. mellifera, but differences in B. terrestris were mainly quantitative. Sequencing of the PCR products revealed a dominance of Alpha-, Beta-, and Gammaproteobacteria, Bacteroidetes, and Firmicutes in all bee species. Single-strand conformation polymorphism profiles suggested a higher abundance and diversity of lactobacilli in adults of A. mellifera than in larvae. Further phylogenetic analyses indicated common bacterial phylotypes for all three bee species, e.g. those related to Simonsiella, Serratia, and Lactobacillus. Clades related to Delftia acidovorans, Pseudomonas aeruginosa or Lactobacillus intestinalis only contained sequences from larvae. Several of the bee-specific clusters also contained identical or highly similar sequences from bacteria detected in other A. mellifera subspecies from South Africa, suggesting the existence of cosmopolitan gut bacteria in bees.

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Section: Introductionmentioning

confidence: 99%

Diversity and phylotype consistency of bacteria in the guts of three bee species (Apoidea) at an oilseed rape field

Mohr

Tebbe

2005

Environmental Microbiology

240

239

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“…Of these isolates from guts, 168 were categorized into 11 bacterial species, including Enterococcuts spp., Serrlatia liquejaciens, Pseiudoinonais spp., and Entelobactier spp. Other investigators reported that hindguts of fifth-instar larvae of turnip moths (Scotia segetuin) contained up to 4 x 105 bacteria, which typically grouped as Enterobacteriaceac and Str-eptococcius ftiecalis, but included 10 species of fungi and a species of yeast (5). The recurrence of certain microbial groups in insects has led some investigators to propose the existence of "entomogenous" bacteria, specific forms that are common in insects and have close ecological interactions with these host insects (11).…”

mentioning

confidence: 99%

The cutworm Peridroma saucia (Lepidoptera: Noctuidae) supports growth and transport of pBR322-bearing bacteria

Armstrong

Porteous

Wood

1989

Appl Environ Microbiol

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Variegated cutworms were exposed to bean plants in microcosms sprayed with pBR322-carrying strains of Enterobacter cloacae, Klebsiella planticola, and Erwinia herbicola. The three bacterial species exhibited differential survival on leaves, in soil, and in guts and fecal pellets (frass) of the insects. High numbers of Enterobacter cloacae(pBR322) were detected in all samples, while the other species were unable to establish residence in the insect. To assess the impact of this colonization on site-to-site transport of microorganisms, larvae were fed plants that had been sprayed with the bacteria and then were transferred to uninoculated plants. Cutworms were efficient carriers of Enterobacter cloacae(pBR322), as indicated by its rapid appearance on uninoculated leaves and continued persistence in the insects for 3 days after transfer. Few Erwinia herbicola(pBR322) and K. planticola(pBR322) were obtained from larvae after transfer, although up to 103 CFU/g were detected in soil and on plants. Differences in bacterial survival and growth were confirmed by incubating frass overnight and observing the change in population numbers. The proportion of total samples showing at least a 25-fold increase during incubation was 68% for Enterobacter cloacae(pBR322), 39% for K. planticola(pBR322), and 0% for Erwinia herbicola(pBR322). Our results emphasize the role that cutworms and possibly other insects have in persistence and growth of microorganisms in the environment.

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“…The digestive system of these larvae is relatively free of common microflora (R. E. Andrews and K. D. Spence, unpublished data). This is by no means the case with many other insects (3,5). For example, common microbes found in the midgut of Scotia segetum (turnip moth) larvae include Streptococcus faecalis, Escherichia coli, Citrobacter freundii, Klebsiella pneumoniae, Proteus spp., Erwinia spp., and Pseudomonas spp.…”

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confidence: 99%

Some Effects of Douglas Fir Terpenes on Certain Microorganisms

Andrews

Parks

Spence

1980

Appl Environ Microbiol

147

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The Douglas fir terpene α-pinene was shown to inhibit the growth of a variety of bacteria and a yeast. Other terpenes of the Douglas fir, including limonene, camphene, and isobornyl acetate, were also inhibitory to Bacillus thuringiensis . All terpenes were inhibitory at concentrations normally present in the fir needle diet of Douglas fir tussock moth larvae. The presence of such terpenes in the diet of these insects was found to strongly influence the infectivity of B. thuringiensis spores for the Douglas fir tussock moth larvae. The terpene α-pinene destroyed the cellular integrity and modified mitochondrial activity in certain microorganisms.

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The bacterial flora of the midgut of two Danish populations of healthy fifth instar larvae of the turnip moth, Scotia segetum

Cited by 11 publications

References 8 publications

Diversity and phylotype consistency of bacteria in the guts of three bee species (Apoidea) at an oilseed rape field

Diversity and phylotype consistency of bacteria in the guts of three bee species (Apoidea) at an oilseed rape field

The cutworm Peridroma saucia (Lepidoptera: Noctuidae) supports growth and transport of pBR322-bearing bacteria

Some Effects of Douglas Fir Terpenes on Certain Microorganisms

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