The Bacterial Heterotrimeric Amidotransferase GatCAB: functions, structures and mechanism-based inhibitors

Pham, Van Hau

doi:10.29328/journal.hjb.1001003

Cited by 4 publications

(1 citation statement)

References 39 publications

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“…GatCAB, an essential heterotrimeric, glutamine-dependent enzyme complex involved in the transamidation of Glu-

and Asp-

, was also investigated. GatA and GatB, subunits of GatCAB that play roles in ammonia production and misacylated tRNA activation and transamidation, respectively, exhibited down-regulation, possibly reducing the formation of Glu-

and Asp-

[ 23 , 24 ]. Furthermore, 14 proteins related to aminoacyl-tRNA biosynthesis were down-regulated, indicating that SAEW treatment might impede peptide chain elongation in protein synthesis ( Tables S2 and S3 ).…”

Section: Discussionmentioning

confidence: 99%

Quantitative Proteomic Analysis on the Slightly Acidic Electrolyzed Water Triggered Viable but Non-Culturable Listeria monocytogenes

Chang,

Gui,

Huang

et al. 2023

IJMS

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This study undertakes a comprehensive exploration of the impact of slightly acidic electrolyzed water (SAEW) on Listeria monocytogenes, a common foodborne pathogen, with a particular focus on understanding the molecular mechanisms leading to the viable but nonculturable (VBNC) state. Given the widespread application of SAEW as an effective disinfectant in the food industry, uncovering these molecular pathways is crucial for improving food safety measures. We employed tandem mass tags (TMT), labeling proteomic techniques and LC-MS/MS to identify differentially expressed proteins under two doses of SAEW conditions. We indicated 203 differential expressed proteins (DEPs), including 78 up-regulated and 125 down-regulated DEPs. The functional enrichment analysis of these proteins indicated that ribosomes, biosynthesis of secondary metabolites, and aminoacyl-tRNA biosynthesis were enriched functions affected by SAEW. Further, we delved into the role of protein chlorination, a potential consequence of reactive chlorine species generated during the SAEW production process, by identifying 31 chlorinated peptides from 22 proteins, with a dominant sequence motif of Rxxxxx[cY] and functionally enriched in translation. Our findings suggest that SAEW might prompt alterations in the protein translation process and trigger compensatory ribosome biosynthesis. However, an imbalance in the levels of elongation factors and AARSs could hinder recovery, leading to the VBNC state. This research carries substantial implications for food safety and sanitation, as it adds to our understanding of the SAEW-induced VBNC state in L. monocytogenes and offers potential strategies for its control.

show abstract

“…GatCAB, an essential heterotrimeric, glutamine-dependent enzyme complex involved in the transamidation of Glu-

and Asp-

Section: Discussionmentioning

confidence: 99%

Quantitative Proteomic Analysis on the Slightly Acidic Electrolyzed Water Triggered Viable but Non-Culturable Listeria monocytogenes

Chang,

Gui,

Huang

et al. 2023

IJMS

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Transcriptomics analysis provides insights into the heat adaptation strategies of an Antarctic bacterium, Cryobacterium sp. SO1

et al. 2023

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A quest for novel antimicrobial targets: Inhibition of Asp-tRNAAsn/Glu-tRNAGln amidotransferase (GatCAB) by synthetic analogs of aminoacyl-adenosine in vitro and live bacteria

Sangsuwan,

Taweesablamlert,

Boonkerd

et al. 2024

Bioorganic Chemistry

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The Bacterial Heterotrimeric Amidotransferase GatCAB: functions, structures and mechanism-based inhibitors

Cited by 4 publications

References 39 publications

Quantitative Proteomic Analysis on the Slightly Acidic Electrolyzed Water Triggered Viable but Non-Culturable Listeria monocytogenes

Quantitative Proteomic Analysis on the Slightly Acidic Electrolyzed Water Triggered Viable but Non-Culturable Listeria monocytogenes

Transcriptomics analysis provides insights into the heat adaptation strategies of an Antarctic bacterium, Cryobacterium sp. SO1

A quest for novel antimicrobial targets: Inhibition of Asp-tRNAAsn/Glu-tRNAGln amidotransferase (GatCAB) by synthetic analogs of aminoacyl-adenosine in vitro and live bacteria

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