The bacterial type VI secretion machine: yet another player for protein transport across membranes

Filloux, Alain; Hachani, Abderrahman; Bleves, Sophie

doi:10.1099/mic.0.2008/016840-0

Cited by 331 publications

(327 citation statements)

References 74 publications

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“…The image is representative of two independent experiments. type IV and type VI secretion systems, may promote host cell membrane ruffling (Angot et al, 2007;Filloux et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Heterogeneity of type III secretion system (T3SS)-1-independent entry mechanisms used by Salmonella Enteritidis to invade different cell types

et al. 2011

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Salmonella causes a wide range of diseases from acute gastroenteritis to systemic typhoid fever, depending on the host. To invade non-phagocytic cells, Salmonella has developed different mechanisms. The main invasion system requires a type III secretion system (T3SS) known as T3SS-1, which promotes a Trigger entry mechanism. However, other invasion factors have recently been described in Salmonella, including Rck and PagN, which were not expressed under our bacterial culture conditions. Based on these observations, we used adhesion and invasion assays to analyse the respective roles of Salmonella Enteritidis T3SS-1-dependent and -independent invasion processes at different times of infection. Diverse cell lines and cell types were tested, including endothelial, epithelial and fibroblast cells. We demonstrated that cell susceptibility to the T3SS-1-independent entry differs by a factor of nine between the most and the least permissive cell lines tested. In addition, using scanning electron and confocal microscopy, we showed that T3SS-1-independent entry into cells was characterized by a Trigger-like alteration, as for the T3SS-1-dependent entry, and also by Zipper-like cellular alteration. Our results demonstrate for what is believed to be the first time that Salmonella can induce Trigger-like entry independently of T3SS-1 and can induce Zipper-like entry independently of Rck. Overall, these data open new avenues for discovering new invasion mechanisms in Salmonella.

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“…The image is representative of two independent experiments. type IV and type VI secretion systems, may promote host cell membrane ruffling (Angot et al, 2007;Filloux et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Heterogeneity of type III secretion system (T3SS)-1-independent entry mechanisms used by Salmonella Enteritidis to invade different cell types

et al. 2011

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“…It suggests that Salmonella express nonidentified invasins able to mediate a Zipper process and factor(s) other than the T3SS-1 that induce Trigger-like invasion process(es). Type IV or type VI secretion systems are good candidates to induce Trigger-like cellular structures as they are able to translocate proteins directly into the host cell cytosol and as they are major virulence determinants involved in the pathogenesis of diverse Gram-negative bacteria (Oliveira et al, 2006;Filloux et al, 2008;Blondel et al, 2010).…”

Section: Non-identified Invasion Factorsmentioning

confidence: 99%

The Different Strategies Used by Salmonella to Invade Host Cells

Rosselin¹,

Abed²,

Namdari³

et al. 2012

Salmonella - Distribution, Adaptation, Control Measures and Molecular Technologies

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“…However, less is known about the organization, function, and mechanism of the T6SS system first discovered by Mekalanos and coworkers in 2006 (3). After their initial report, a number of bioinformatics-based comparative analyses of the T6SS gene clusters between bacterial strains have been made to assess possible function (4)(5)(6)(7)(8)(9)(10). In this issue of PNAS, both Leiman et al (11) and Pell et al (12) use structure-based analysis to demonstrate the existence of a structure/function relationship between the molecular components of T6SS and the tail proteins of bacteriophages T4 (11) and (12).…”

mentioning

confidence: 99%

Structural similarity of tailed phages and pathogenic bacterial secretion systems

Kanamaru¹

2009

Proc. Natl. Acad. Sci. U.S.A.

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The bacterial type VI secretion machine: yet another player for protein transport across membranes

Cited by 331 publications

References 74 publications

Heterogeneity of type III secretion system (T3SS)-1-independent entry mechanisms used by Salmonella Enteritidis to invade different cell types

Heterogeneity of type III secretion system (T3SS)-1-independent entry mechanisms used by Salmonella Enteritidis to invade different cell types

The Different Strategies Used by Salmonella to Invade Host Cells

Structural similarity of tailed phages and pathogenic bacterial secretion systems

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