The Bactericidal Effect of 2780 and 940 nm Laser Irradiation on<i>Enterococcus faecalis</i>in Bovine Root Dentin Slices of Different Thicknesses

Gutknecht, Norbert; Al-Karadaghi, Tamara Sardar; Al‐Maliky, Mohammed Abbood; Conrads, Georg; Franzen, René

doi:10.1089/pho.2015.3960

Cited by 29 publications

(18 citation statements)

References 36 publications

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“…Therefore, it is recommended that similar studies be carried out in curved root canals, too, to make it possible to extend the results to real clinical conditions. Furthermore, in previous studies, application of lasers, photodynamic therapy and agitation of the irrigation solution with ultrasonic devices have drawn attention as antimicrobial adjuncts and it has been reported that they increase penetration of irrigation solutions into more apical areas and root canal irregularities (20,28). It is suggested that the efficacy of these procedures in association with NaOCl, as the most effective canal irrigation solution, be evaluated in relation to old and mature biofilms and mixed bacterial species of the root canal system so that the best antimicrobial protocol can be identified.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial efficacy of different concentration of sodium hypochlorite on the biofilm of Enterococcus faecalis at different stages of development

Froughreyhani¹,

Ghasemi²,

Barhaghi³

et al. 2016

J Clin Exp Dent

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BackgroundPersistent infection of the root canal due to the presence of resistance bacterial species, such as Enterococcus faecalis, has always been one of the most important reasons for endodontic treatment failure. This study investigated the antimicrobial efficacy of 1%, 2.5 % and 5% sodium hypochlorite in eliminating E. faecalis biofilms at different stages of development.Material and MethodsIn this study 4-, 6- and 10-week-old E. faecalis biofilms were subjected to one of the following approaches: phosphate-buffered saline solution (PBS) or 1%, 2.5% and 5% NaOCl. Dentin chip suspensions were used for colony forming unit (CFU) counting to estimate remaining E. faecalis counts. Statistical comparison of the means was carried out with Kruskal-Wallis test, and pair-wise comparisons were made by Mann-Whitney U test, at a significance level of P<0.05.ResultsThe results showed that 2.5% and 5% NaOCl completely eliminated E. faecalis biofilms in three stages of biofilm development, whereas 1% NaOCl resulted in 85.73%, 81.88% and 78.62% decreases in bacterial counts in 4-, 6- and 10-week-old biofilms, respectively, which was significantly more than those with PBS (p<0.05).Conclusions The bacteria in mature and old biofilms were more resistant to 1% NaOCl than were the bacteria in young biofilms. Overall survival rate and residual bacteria increased with biofilm aging. Key words:Antibacterial, biofilm, E. faecalis, sodium hypochlorite.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial efficacy of different concentration of sodium hypochlorite on the biofilm of Enterococcus faecalis at different stages of development

Froughreyhani¹,

Ghasemi²,

Barhaghi³

et al. 2016

J Clin Exp Dent

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“…Unlike the conventional method, lasers of suitable wavelengths have an excellent capacity for bacterial reduction at elimination depths exceeding 1000 μm in deep dentin tubules [13,21,22]. However, despite the potential benefits of lasers, too high, a dose of the absorbed energy may cause thermal damage to periodontal ligaments and lad to bone necrosis [15,23,24].…”

Section: Introductionmentioning

confidence: 99%

Temperature elevation during root canal treatment with a 445-nm diode laser—an in vitro study

2018

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Objective This study investigated the thermal effects of a 445-nm diode laser on tooth roots of endodontic treatment. Material and methods A total of 15 human single-rooted extracted teeth were included in the survey. Samples were sectioned at the cementoenamel junction. The root canals were instrumented using a rotary system. Each root was fitted into a resin mold and was fixed in a vertical position using clamps. All samples were placed on a support in a thermal path at 37°C. The root canals were randomly divided into three groups (n = 5 each): samples were irradiated with 0.6 W continuous wave (CW), 0.4 W CW, and 1.2 W gated mode, at a repetition rate of 10 Hz with a pulse duration of 50 ms. The time-dependent temperature elevations of the cervical, middle, and apical thirds of the roots were recorded at a sampling rate of 2 Hz during laser irradiation with a K-type thermocouple measurement system. Results The mean temperature rise inside the roots in this study was below 10°C, whereas the maximum temperature rise of 9.83°C (0.6 W CW) and 9.81°C (1.2 W gated mode, repetition rate 10 Hz, and pulse duration 50 ms) was registered in lower incisors, and 4.97°C was detected at 0.4 W CW. The thermal changes seen in the apical thirds of the root were statically significantly (p < 0.001) and were greater than those at the coronal and middle thirds. Conclusion The results of this study suggest that 445-nm diode lasers may provide a safe temperature for endodontic applications.

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“…Endodontia tem evoluído com objetivo de promover a desinfecção do sistema de canais radiculares (Hmud, Kahler et al, 2010;Asnaashari e Safavi, 2013;Gutknecht, Al-Karadaghi et al, 2016). O laser diodo em diferentes comprimentos de onda (970nm e 808nm) foi desenvolvido para aplicação em Endodontia, apresentando fibras ópticas finas, com 200-320 µm de diâmetro, que permitem adaptação às dimensões reduzidas e curvaturas dos canais radiculares, possibilitando a descontaminação ao longo do conduto (Brugnera, Zanin et al, 2003), bem como a ação do laser no terço apical do canal radicular (Beer, Buchmair et al, 2012;Maenosono, Bim Junior et al, 2015;Lopes, Roperto et al, 2016).…”

Section: Dentro Desse Contexto O Potencial De Aplicação Do Laser No unclassified

“…Com a finalidade de promover a descontaminação efetiva do SCR, terapias alternativas e coadjuvantes ao tratamento endodôntico foram propostas, como o uso de diferentes tipos de lasers (HMUD et al, 2010;ASNAASHARI et al, 2013;GOMES et al, 2013;ARNEIRO et al, 2014;LLENA et al, 2015;GUTKNECHT et al, 2016;MORAGO et al, 2016), entre eles o laser diodo, que ao atuar em camadas mais profundas da dentina (ALFREDO et al, 2009), permite a redução dos microrganismos no interior do SCR (SCHOOP et al, 2006;KANUMURU et al, 2014;MASHALKAR et al, 2014;ASNAASHARI et al, 2016).…”

Section: Análise Estatísticaunclassified

“…A ação antibacteriana do laser diodo 970nm está diretamente relacionada ao aquecimento do substrato dentinário ao qual as bactérias estão ligadas (KANUMURO et al, 2014;GUTKNECHT et al, 2016), e à interação de um sensibilizador fotoativado com o substrato biológico, o que leva à produção de oxigênio altamente reativo, que pode danificar moléculas celulares essenciais, incluindo proteínas, lipídios da membrana e ácido nucleico (BAGO et al, 2013;ASNAASHARI et al, 2016). Segundo SCHOOP et al (2006), ao avaliarem o efeito antibacteriano do laser diodo 980nm, evidenciaram redução significativa no número de bactérias, principalmente quando irradiado com potência de 1,5W.…”

Section: Análise Estatísticaunclassified

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Efeito de diferentes comprimentos de onda do laser diodo na descontaminação de dentina radicular infectada com <i>Enterococcus Faecalis</i>

Borges¹

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Ao meu orientador, Prof. Dr. Jesus Djalma Pécora, exemplo de dedicação e perseverança, pelos ensinamentos e orientações durante o desenvolvimento deste projeto. Ao Prof. Dr. Carlos Estrela, por todo incentivo e direcionamento durante o desenvolvimento deste projeto, por todas as orientações, por exercer papel fundamental na minha formação, desde minha graduação e contribuído para minha escolha por esta especialidade. Ao Prof. Dr. Manoel Damião de Sousa Neto, pela oportunidade da realização do curso de mestrado, pelos conhecimentos compartilhados que exerceram um papel fundamental na minha formação científica. À Profa. Dra. Cyntia Rodrigues De Araújo Estrela por todo direcionamento e conhecimento passado durante o trabalho experimental. Agradeço imensamente a grande atenção, disponibilidade e dedicação. À Profa. Dra. Regina Guenka Palma Dibb, exemplo de trabalho, sempre disposta a passar seus conhecimentos. Muito obrigada pela abertura de realizar discussões, tirar dúvidas, pela agradável convivência, e pelo constante aprendizado.

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The Bactericidal Effect of 2780 and 940 nm Laser Irradiation onEnterococcus faecalisin Bovine Root Dentin Slices of Different Thicknesses

Abstract: The results of this in vitro study showed that the dual wavelength laser system obtained a significantly higher bactericidal effect on E. faecalis than Er,Cr:YSGG laser, reaching a depth of 1000 μm of dentin.

Cited by 29 publications

References 36 publications

Antimicrobial efficacy of different concentration of sodium hypochlorite on the biofilm of Enterococcus faecalis at different stages of development

Antimicrobial efficacy of different concentration of sodium hypochlorite on the biofilm of Enterococcus faecalis at different stages of development

Temperature elevation during root canal treatment with a 445-nm diode laser—an in vitro study

Efeito de diferentes comprimentos de onda do laser diodo na descontaminação de dentina radicular infectada com <i>Enterococcus Faecalis</i>

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