2018
DOI: 10.3390/v10070343
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The Bacteriophage T4 MotB Protein, a DNA-Binding Protein, Improves Phage Fitness

Abstract: The lytic bacteriophage T4 employs multiple phage-encoded early proteins to takeover the Escherichia coli host. However, the functions of many of these proteins are not known. In this study, we have characterized the T4 early gene motB, located in a dispensable region of the T4 genome. We show that heterologous production of MotB is highly toxic to E. coli, resulting in cell death or growth arrest depending on the strain and that the presence of motB increases T4 burst size 2-fold. Previous work suggested that… Show more

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Cited by 8 publications
(22 citation statements)
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“…Deletion of the motB gene led to a decreased burst size [76]. The T4 protein Arn represents an interesting example of a phage-encoded DNA mimic protein and was shown to directly interact with E. coli H-NS [77].…”
Section: How To Overcome Silencing?mentioning
confidence: 99%
“…Deletion of the motB gene led to a decreased burst size [76]. The T4 protein Arn represents an interesting example of a phage-encoded DNA mimic protein and was shown to directly interact with E. coli H-NS [77].…”
Section: How To Overcome Silencing?mentioning
confidence: 99%
“…One such gene is the phage T4 motB , which encodes an early gene product. Although nonessential under normal laboratory conditions, our previous work has demonstrated that a motB knockdown yields a 2-fold lower burst than wild type (WT) T4, indicating that it contributes to phage fitness and that purified MotB binds tightly to both unmodified and T4 modified (5-glucosylated, hydroxymethylated cytosine) DNA [ 2 ]. Given that motB encodes an early protein, these results would be consistent with a role for motB in T4 gene expression, as is seen for the T4 early genes asiA and motA , which are required for middle gene activation [ 3 ].…”
Section: Introductionmentioning
confidence: 99%
“…In fact, motB was named as a modifier of transcription, from very early work suggesting that a large T4 genomic deletion that removed motB affects some T4 middle gene expression [ 4 ]. However, our global transcriptomic analyses have demonstrated that a T4 motB knockdown does not significantly affect T4 RNA levels [ 2 ].…”
Section: Introductionmentioning
confidence: 99%
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