The Baculovirus 10-kDa Protein

Oers, Monique M. van; Vlak, Just M.

doi:10.1006/jipa.1997.4675

Cited by 55 publications

(34 citation statements)

References 103 publications

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“…Sequence analysis of the p74 gene region also revealed the presence of an ORF (Cf-xiv) containing 315 nucleotides with a homology to the baculoviral fibrillin proteins. This protein owes its name to its fibrillar structure (van Oers and Vlak, 1997), and is located adjacent to the p74 gene, which encodes a highly conserved viral envelope protein. The presumed ChfuGV fibrillin gene codes a 104 amino acid protein with a molecular weight equal to 11811 Da.…”

Section: Resultsmentioning

confidence: 99%

Identification, Characterization and Phylogenic Analysis of Conserved Genes within the p74 Gene Region of Choristoneura fumiferana Granulovirus Genome

Rashidan¹,

Nassoury²,

Giannopoulos³

et al. 2004

BMB Reports

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Section: Resultsmentioning

confidence: 99%

Identification, Characterization and Phylogenic Analysis of Conserved Genes within the p74 Gene Region of Choristoneura fumiferana Granulovirus Genome

Rashidan¹,

Nassoury²,

Giannopoulos³

et al. 2004

BMB Reports

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“…With ClustalW, nl53 showed the highest amino acid identity to the Epiphyas postvittana MNPV (36) P10, but this was only 13.8%. Amino acid identity, however, is usually low for P10 proteins, and AcMNPV P10, for example, shares only 20.1% amino acid identity with SpltMNPV P10 (65). We cannot, therefore, rule out the possibility that nl53 may be a P10 homologue.…”

Section: Vol 78 2004 N Lecontei Npv Genome 7029mentioning

confidence: 95%

“…P10 is a very late protein that is generally poorly conserved at the amino acid level, but its size, hydrophilicity distribution, and secondary structure are conserved (72). Four structural domains implicated in various functions are usually found, including a coiled-coil domain at the amino-terminal end, followed by a proline-rich domain, a variable region not found in all P10s, and a positively charged carboxy terminus often containing serine or threonine residues (63,64,65,68). Known P10 proteins range in size from 70 aa for Bombyx mori NPV (BmNPV) (25) to 105 aa for SpltMNPV (50) and are usually located between p26 and p74 (65).…”

Section: Vol 78 2004 N Lecontei Npv Genome 7029mentioning

confidence: 99%

“…Four structural domains implicated in various functions are usually found, including a coiled-coil domain at the amino-terminal end, followed by a proline-rich domain, a variable region not found in all P10s, and a positively charged carboxy terminus often containing serine or threonine residues (63,64,65,68). Known P10 proteins range in size from 70 aa for Bombyx mori NPV (BmNPV) (25) to 105 aa for SpltMNPV (50) and are usually located between p26 and p74 (65). Nl53 showed a weak blastp match with P10 from Buzura suppressaria NPV (BusuNPV) (34), showing 47% identity over 26 of 54 amino acids, but amino acid identity over the entire ORF was much lower.…”

Section: Vol 78 2004 N Lecontei Npv Genome 7029mentioning

confidence: 99%

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Sequence and Organization of the Neodiprion lecontei Nucleopolyhedrovirus Genome

Lauzon

Lucarotti

Krell

et al. 2004

J Virol

133

124

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All fully sequenced baculovirus genomes, with the exception of the dipteran Culex nigripalpus nucleopolyhedrovirus (CuniNPV), have previously been from Lepidoptera. This study reports the sequencing and characterization of a hymenopteran baculovirus, Neodiprion lecontei nucleopolyhedrovirus (NeleNPV), from the redheaded pine sawfly. NeleNPV has the smallest genome so far published (81,755 bp) and has a GC content of only 33.3%. It contains 89 potential open reading frames, 43 with baculovirus homologues, 6 identified by conserved domains, and 1 with homology to a densovirus structural protein. Average amino acid identity of homologues ranged from 19.7% with CuniNPV to 24.9% with Spodoptera exigua nucleopolyhedrovirus. The conserved set of baculovirus genes has dropped to 29, since NeleNPV lacks an F protein homologue (ac23/ ld130). NeleNPV contains 12 conserved lepidopteran baculovirus genes, including that for DNA binding protein, late expression factor 11 (lef-11), polyhedrin, occlusion derived virus envelope protein-18 (odv-e18), p40, and p45, but lacks 21 others, including lef-3, me53, immediate early gene-1, lef-6, pp31, odv-e66, few polyhedra 25k, odv-e25, protein kinase-1, fibroblast growth factor, and ubiquitin. The lack of identified baculovirus homologues may be due to difficulties in identification, differences in host-virus interactions, or other genes performing similar functions. Gene parity plots showed limited colinearity of NeleNPV with other baculoviruses, and phylogenetic analysis indicates that NeleNPV may have existed before the lepidopteran nucleopolyhedrovirus and granulovirus divergence. The creation of two new Baculoviridae genera to fit hymenopteran and dipteran baculoviruses may be necessary.

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“…A phosphorylated protein encoded by the pep (polyhedral envelope protein) gene is a structural component of this envelope (Whitt & Manning, 1988). Also, the p10 gene appears to be involved in the formation of the calyx, though it is not itself a component of the calyx (Van Oers & Vlak, 1997). The calyx appears to lend physical stability to the OBs, but it has also been speculated that the calyx serves to delimit the boundaries of OBs and define their shape (Eason et al, 1998).…”

Section: Aberrant Ob Morphology In Other Baculovirusesmentioning

confidence: 99%

Isolation of an Adoxophyes orana granulovirus (AdorGV) occlusion body morphology mutant: biological activity, genome sequence and relationship to other isolates of AdorGV

Nakai

Harrison

Uchida

et al. 2015

Journal of General Virology

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A granulovirus (GV) producing occlusion bodies (OBs) with an unusual appearance was isolated from Adoxophyes spp. larvae in the field. Ultrastructural observations revealed that its OBs were significantly larger and cuboidal in shape, rather than the standard ovo-cylindrical shape typical of GVs. N-terminal amino acid sequence analysis of the OB matrix protein from this virus suggested that this new isolate was a variant of Adoxophyes orana granulovirus (AdorGV). Bioassays of this GV (termed AdorGV-M) and an English isolate of AdorGV (termed AdorGV-E) indicated that the two isolates were equally pathogenic against larvae of Adoxophyes honmai. However, AdorGV-M retained more infectivity towards larvae after irradiation with UV light than did AdorGV-E. Sequencing and analysis of the AdorGV-M genome revealed little sequence divergence between this isolate and AdorGV-E. Comparison of selected genes among the two AdorGV isolates and other Japanese AdorGV isolates revealed differences that may account for the unusual OB morphology of AdorGV-M.

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The Baculovirus 10-kDa Protein

Cited by 55 publications

References 103 publications

Identification, Characterization and Phylogenic Analysis of Conserved Genes within the p74 Gene Region of Choristoneura fumiferana Granulovirus Genome

Identification, Characterization and Phylogenic Analysis of Conserved Genes within the p74 Gene Region of Choristoneura fumiferana Granulovirus Genome

Sequence and Organization of the Neodiprion lecontei Nucleopolyhedrovirus Genome

Isolation of an Adoxophyes orana granulovirus (AdorGV) occlusion body morphology mutant: biological activity, genome sequence and relationship to other isolates of AdorGV

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