2000
DOI: 10.1096/fasebj.14.2.242
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The balance sheet for transcription: an analysis of nuclear RNA metabolism in mammalian cells

Abstract: The control of RNA synthesis from protein-coding genes is fundamental in determining the various cell types of higher eukaryotes. The activation of these genes is driven by promoter complexes, and RNA synthesis is performed by an enzyme mega-complex-the RNA polymerase II holoenzyme. These two complexes are the fundamental components required to initiate gene expression and generate the primary transcripts that, after processing, yield mRNAs that pass to the cytoplasm where protein synthesis occurs. But althoug… Show more

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Cited by 144 publications
(103 citation statements)
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“…We can imagine a range of possibilities, from each site containing one unit (with 6 -17 engaged polymerases) to 6 -17 units (each with one engaged enzyme). The latter is consistent with our previous results (Cook, 1999;2002), and with observations that few eukaryotic units are associated with more than one engaged polymerase at any moment (reviewed by Jackson et al, 2000). Moreover, microarray analyses reveal only 73 of the several thousand active open reading frames in yeast are typically associated with one or more engaged polymerases (Bon et al, 2006b).…”
Section: Number Of Active Polymerases Per Sitesupporting
confidence: 92%
“…We can imagine a range of possibilities, from each site containing one unit (with 6 -17 engaged polymerases) to 6 -17 units (each with one engaged enzyme). The latter is consistent with our previous results (Cook, 1999;2002), and with observations that few eukaryotic units are associated with more than one engaged polymerase at any moment (reviewed by Jackson et al, 2000). Moreover, microarray analyses reveal only 73 of the several thousand active open reading frames in yeast are typically associated with one or more engaged polymerases (Bon et al, 2006b).…”
Section: Number Of Active Polymerases Per Sitesupporting
confidence: 92%
“…S6 and Materials and Methods). Moreover, the density of RNAP II molecules observed was found to be 37.2 ± 4.1 molecules per ÎŒm 3 (n = 12,482 molecules in eight cells), which translates to a total of 80,200 ± 8,800 RNAP II molecules in the entire nucleus (Materials and Methods), in good agreement with values reported in previous studies (13,15,37). Therefore, we conclude that we indeed exhaustively imaged most of the labeled RNAP II molecules in the optical section illuminated.…”
Section: One-to-one Labeling and Superresolution Imaging Of Rnap II Msupporting
confidence: 91%
“…Of all the RNA transcribed in human nuclei, only about 5% enters the cytoplasm 72 . Quality control mechanisms, such as nonsense-mediated decay, dispose of incompletely or improperly processed messages encoding flawed proteins 73 .…”
Section: Rna-directed Rewriting Of Rnamentioning
confidence: 99%