2022
DOI: 10.1101/2022.03.11.484019
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The Balbiani body is formed by microtubule-controlled molecular condensation of Buc in early oogenesis

Abstract: Molecular condensates provide new paradigms in biology, but their cellular regulation is unclear. Condensates undergo phase separation, decreasing their solubility and compartmentalizing their content. In vertebrate oocytes, RNA-protein (RNP) granules form condensates by phase separation, but the underlying mechanisms are unknown. RNP granules localize to the Balbiani body (Bb), a conserved membraneless organelle, that establishes oocyte polarity. Bb loss results in symmetrical eggs and embryonic lethality. Bb… Show more

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Cited by 2 publications
(2 citation statements)
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References 159 publications
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“…Additional advantages of the zebrafish model include the high accessibility of developing ovaries that are present in swimming juvenile fish, and the flat and transparent anatomy of the ovary, which is ideal for advanced quantitative and live microscopy, as we demonstrated previously ( Elkouby and Mullins, 2017a , b ; Elkouby et al, 2016 ; Mytlis and Elkouby, 2021 ; Deis and Elkouby, 2022 preprint; Mytlis et al, 2022 ), and in this work. Furthermore, in zebrafish germline stem cells are maintained and actively produce oocytes throughout life and during regeneration ( Beer and Draper, 2013 ; Cao, et al, 2019 ).…”
Section: Discussionmentioning
confidence: 58%
See 1 more Smart Citation
“…Additional advantages of the zebrafish model include the high accessibility of developing ovaries that are present in swimming juvenile fish, and the flat and transparent anatomy of the ovary, which is ideal for advanced quantitative and live microscopy, as we demonstrated previously ( Elkouby and Mullins, 2017a , b ; Elkouby et al, 2016 ; Mytlis and Elkouby, 2021 ; Deis and Elkouby, 2022 preprint; Mytlis et al, 2022 ), and in this work. Furthermore, in zebrafish germline stem cells are maintained and actively produce oocytes throughout life and during regeneration ( Beer and Draper, 2013 ; Cao, et al, 2019 ).…”
Section: Discussionmentioning
confidence: 58%
“…Owing to multiple experimental advantages, the zebrafish ovary is as an excellent model for the study of oogenesis ( Elkouby and Mullins, 2017b ; Li and Ge, 2020 ), and the toolbox for the investigation of zebrafish oogenesis has been significantly expanded. Advances in genetics ( Jamieson-Lucy et al, 2022 ; Leerberg et al, 2019 ; Beer and Draper, 2013 ), quantitative and live ovarian imaging ( Mytlis et al, 2022 ; Mytlis and Elkouby, 2021 ), live manipulations of cultured ovaries ( Deis and Elkouby, 2022 preprint), and various proteomic and genomic approaches ( Jamieson-Lucy et al, 2022 ; Liu et al, 2022 ; Bogoch et al, 2022 ) have impacted the field greatly. Nevertheless, a fundamental understanding of the cyst is still needed.…”
Section: Introductionmentioning
confidence: 99%