The Basal Cells in the Papillae Fungiformes of the Tongue of the Common Frog, &lt;i&gt;Rana temporaria&lt;/i&gt; L.

Goossens, N.; Vandenberghe, M. P.

doi:10.1679/aohc1950.36.173

Cited by 18 publications

(6 citation statements)

References 21 publications

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“…The presence of biogenic monoamines in taste buds of frogs, fish, and rabbits has been investigated by the Falck-Hillarp fluorescence histochemical method (DeHan and Graziadei, 1973;Savushkina et al, 1974;Goossens and Vandenberghe, 1974;Nada and Hirata, 1975;Fujimoto et al, 1987). However, there has been controversy about the Fig.…”

Section: Discussionmentioning

confidence: 99%

Localization of serotonin in taste buds: A comparative study in four vertebrates

Kim

Roper

1995

J of Comparative Neurology

101

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To investigate monoaminergic synaptic mechanisms in taste buds, we examined taste buds of mice, rats, rabbits, and mudpuppies for the presence of the neurotransmitter candidate, serotonin. Immunocytochemistry revealed serotonin-like immunostaining in cells in mammalian taste buds and Merkel-like basal cells in taste buds of mudpuppies. In untreated mudpuppies and in mammals injected with the precursor to serotonin, L-tryptophan, certain taste cells showed serotonin-like immunoreactivity, although in mammalian taste buds the immunostaining was relatively weak. After pretreating mammals with 5-hydroxytryptophan (5-HTP), the intermediate precursor between L-tryptophan and serotonin, several taste cells showed strong immunoreactivity for serotonin. These findings indicate that mammalian taste cells normally contain serotonin and that taste cells can take up 5-HTP and convert it to serotonin. Immunocytochemistry on wholemount preparations demonstrated that serotonergic cells of mudpuppies (i.e., Merkel-like basal cells) were disposed in a ring at the periphery of taste buds. Similarly, serotonergic cells in mammalian taste buds tended to be located at the periphery of taste buds. Based on the position of serotonergic cells in the taste bud and on recent physiological studies on the actions of serotonin in taste buds, we postulate that serotonin functions as a neuromodulator or neurotransmitter in vertebrate taste buds.

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Section: Discussionmentioning

confidence: 99%

Localization of serotonin in taste buds: A comparative study in four vertebrates

Kim

Roper

1995

J of Comparative Neurology

101

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“…In addition, both cell types form synapses with innervating nerve fibers and some of the synapses appear to be reciprocal (Reutter, 197 1, 1978(Reutter, 197 1, , 1986Parducz et al, 1977;Delay and Roper, 1988). Further, taste buds are believed to have mechanoreceptive function in addition to their established chemosensitivity (Goossens and Vandenberghe, 1974;During and Andres, 1976;Toyoshima et al, 1984) and it has seemed plausible to impute Merkel-like basal cells as the mechanosensory modality. However, some investigators have argued that these structural similarities are not enough to equate the two cell types (Munger and Ide, 1988;Whitear, 1989;Munger, 1993).…”

Section: Discussionmentioning

confidence: 99%

Membrane properties of two types of basal cells in Necturus taste buds

1994

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Necturus taste buds contain two types of basal cells: presumptive stem cells and Merkel-like basal cells. Both types of basal cells are small round cells located at the base of the taste bud, indistinguishable from each other with light microscopy. However, with electron microscopy, autoradiography, or immunocytochemistry, these two types of basal cells can be easily distinguished. We isolated basal cells from taste buds, characterized their voltage-dependent currents using gigaseal whole-cell recordings, and processed the cells for electron microscopy or immunocytochemistry. We were able to distinguish two cell types electrophysiologically and to correlate cell type with membrane properties. Isolated Merkel-like basal cells had several voltage- activated currents: transient, TTX-sensitive, inward Na+ current; sustained, saturating outward K+ current; and slowly inactivating inward Ca2+ current. These currents are similar to those observed in taste receptor cells. In contrast, presumptive stem cells from Necturus taste buds only had outward K+ currents.

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“…By formaldehyde-induced histofluorescence and immunocytochemistry, numerous investigators identifi-ed 5HT in mammalian taste buds and in frog taste discs (GoosENS and VANDERBERGHE, 1974;FUJIMOTO et al, 1987;KIM and ROPER, 1995). Recently, 5HT-like immunoreactivity has been found in taste cells of the rat, mouse and rabbit after administration of the 5HT precursor, 5-hydroxytryptophan (5HTP) (KIM and ROPER, 1995).…”

Section: Serotonin (5ht)mentioning

confidence: 99%

Immunohistochemical Studies on Protein Gene Product 9.5, Serotonin and Neuropeptides in Vallate Taste Buds and Related Nerves of the Guinea Pig.

1996

Archives of Histology and Cytology

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The Basal Cells in the Papillae Fungiformes of the Tongue of the Common Frog, <i>Rana temporaria</i> L.

Cited by 18 publications

References 21 publications

Localization of serotonin in taste buds: A comparative study in four vertebrates

Localization of serotonin in taste buds: A comparative study in four vertebrates

Membrane properties of two types of basal cells in Necturus taste buds

Immunohistochemical Studies on Protein Gene Product 9.5, Serotonin and Neuropeptides in Vallate Taste Buds and Related Nerves of the Guinea Pig.

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