The Base Sequence Corresponding to DYZ1 is Detectable in the DNA from Many Animal Species.

Higashi, Yohsuke; Iwaya, Makoto; Saga, Masahiko; Ogawa, Shyoso

doi:10.1262/jrd.38.55

Cited by 1 publication

(1 citation statement)

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“…On the other hand, use of a polymerase chain reaction (PCR) with a male-specific sequence [28] simplifies the sexing process because the PCR method is not influenced by the culture conditions of the sexing sample. The sexing rate of PCR has accordingly been shown to be equivalent to that of a chromosomal analysis [29], and the substitution of a male-specific sequence with a commerciallyavailable repetitive DNA primer [30] achieved a high sex prediction rate (64/69, 93%), even for a sexing sample of less than 10 blastomeres.…”

Section: Embryo Sexingmentioning

confidence: 99%

Application Study of Developmental Engineering for Livestock Production

Ushijima

2005

J. Reprod. Dev.

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Abstract. This paper describes several technical improvements in developmental engineering for livestock production, including their practical utility in the field. The artificial production of monozygotic twins via embryo splitting is shown to increase embryo productivity, while embryo sexing capability provides added value without compromising offspring productivity, with both techniques being adequate for practical field applications. It is also shown that: (1) the development of nuclear transfer utilizing oocytes collected from slaughtered ovaries and matured in vitro enables producing a large number of cloned embryos, (2) the intracytoplasmic injection of somatic cell improves the productivity of nuclear transplantation, and (3) the injection of sperm increases the rate of normal oocytes with male and female pronuclei allowing further preimplantation development. Finally, the removal of cytoplasmic lipid droplets from embryos following centrifugation alters an embryo's intrinsic sensitivity to low temperature allowing long-term preservation. Collectively, these techniques have clearly provided improvements in developmental engineering for livestock production.

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Section: Embryo Sexingmentioning

confidence: 99%