The basic helix-loop-helix transcription factor bHLH95 affects fruit ripening and multiple metabolisms in tomato

Zhang, Lincheng; Kang, Jing; Xie, Qiaoli; Gong, Jun; Shen, Hui; Chen, Yanan; Chen, Guoping; Hu, Zongli

doi:10.1093/jxb/eraa363

Cited by 44 publications

(13 citation statements)

References 55 publications

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“…Among these genes, 13 members of the bHLH gene family and 12 members of the GRAS family undergo significant changes in their transcript levels during the early stages of ripening ( Figure 1D; Supplemental Table S1 ). This observation is in line with the reported downregulation of SlbHLH95 that affects accumulation of total carotenoids, lycopene, as well as ethylene sensitivity ( Zhang et al, 2020 ). Likewise, silencing of SlGRAS38 results in lower lycopene content and reduced ethylene production in tomato fruit, while the overexpression of SlGRAS4 leads to premature ripening ( Liu et al, 2021 ).…”

Section: Discussionsupporting

confidence: 90%

Transition to ripening in tomato requires hormone-controlled genetic reprogramming initiated in gel tissue

et al. 2022

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Ripening is the last stage of the developmental program in fleshy fruits. During this phase, fruits become edible and acquire their unique sensory qualities and post-harvest potential. Although our knowledge of the mechanisms that regulate fruit ripening has improved considerably over the past decades, the processes that trigger the transition to ripening remain poorly deciphered. While transcriptomic profiling of tomato (Solanum lycopersicum L.) fruit ripening to date has mainly focused on the changes occurring in pericarp tissues between the Mature Green and Breaker stages, our study addresses the changes between the Early Mature Green and Late Mature Green stages in the gel and pericarp separately. The data showed that the shift from an inability to initiate ripening to the capacity to undergo full ripening requires extensive transcriptomic reprogramming that takes place first in the locular tissues before extending to the pericarp. Genome-wide transcriptomic profiling revealed the wide diversity of transcription factor families engaged in the global reprogramming of gene expression and identified those specifically regulated at the Mature Green stage in the gel but not in the pericarp, thereby providing potential targets towards deciphering the initial factors and events that trigger the transition to ripening. The study also uncovered an extensive reformed homeostasis for most plant hormones, highlighting the multi-hormonal control of ripening initiation. Our data unveil the antagonistic roles of ethylene and auxin during the onset of ripening and show that auxin treatment delays fruit ripening via impairing the expression of genes required for System-2 autocatalytic ethylene production that is essential for climacteric ripening. This study unveils the detailed features of the transcriptomic reprogramming associated with the transition to ripening of tomato fruit and shows that the first changes occur in the locular gel before extending to pericarp and that a reformed auxin homeostasis is essential for the ripening to proceed.

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Section: Discussionsupporting

confidence: 90%

Transition to ripening in tomato requires hormone-controlled genetic reprogramming initiated in gel tissue

et al. 2022

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“…More importantly, there was a positive correlation between the expression level of Cla017389 and the contents of lycopene (r =0.85), as mentioned above. The transcription factor bHLH is related to the carotenoid metabolism in tomato [34], papaya [35], and citrus [36]. In this study, the expression of Cla006599 and Cla022119 (bHLH) were decreased at 20 and 34 DAP as compared to 10 DAP, and their expression patterns were similar to CpbHLH1/2 that regulate carotenoid biosynthesis in papaya [35] (Additional le 2: Fig.…”

Section: Identi Cation Of Differentially Expressed Genes In Ve Genotypesmentioning

confidence: 60%

Transcriptome regulation of carotenoids in five flesh-colored watermelon (Citrullus lanatus)

Yuan

Umer

et al. 2021

Preprint

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Background: Fruit flesh color in watermelon (Citrullus lanatus) is a great index for evaluation of the appearance quality and a key contributor influencing consumers' preferences, but the molecular mechanism of this intricate trait remain largely unknown. Here, the carotenoids and transcriptome dynamics during the fruit development of cultivated watermelon with five different flesh colors were analyzed.Results: A total of 13 carotenoids and 16781 differentially expressed genes (DEGs) including 1295 transcription factors (TFs) were detected in five watermelon genotypes during the fruit development. The comprehensive accumulation patterns of carotenoids were closely related to flesh color. A number of potential structural genes and transcription factors were found to be associated with the carotenoid biosynthesis pathway using comparative transcriptome analysis. The differentially expressed genes were divided into six subclusters and distributed in different GO terms and metabolic pathways. Furthermore, we performed weighted gene co-expression network analysis and predicted hub genes in six main modules determining carotenoid contents. Cla018406 (a chaperone protein dnaJ-like protein) may be a candidate gene for β-carotene accumulation and highly expressed in orange flesh-colored fruit. Cla007686 (a zinc finger CCCH domain-containing protein) was highly expressed in the red flesh-colored watermelon, maybe a key regulator of lycopene accumulation. Cla003760 (membrane protein) and Cla021635 (photosystem I reaction center subunit II) were predicted to be hub genes and may play an essential role in yellow flesh formation.Conclusions: The composition and contents of carotenoid in five watermelon genotypes vary greatly. A series of candidate genes were revealed through combined analysis of metabolites and transcriptome. These results provide an important data resource for dissecting the candidate genes and molecular basis governing flesh color formation in watermelon fruit.

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“…2). SlbHLH95 also was proved to be participated in the regulation of fruit ripening through ethylene metabolism pathway (Zhang et al 2020). In addition, co-expression network analysis found that several transcription factors including NAC, WRKY and bHLH showed co-expression relationship with VvUFD1 genes, suggesting they might be regulators of the expression of the VvUFD1 genes (Fig.…”

Section: The Vvufd1 Genes Might Play Roles In Fruit Development and Ripeningmentioning

confidence: 99%

Genome-wide identification and characterization of grapevine UFD1 genes during berry development and salt stress response

Wei

Jiaan

Jiang

et al. 2022

J. Plant Biochem. Biotechnol.

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Grapevine (Vitis vinifera) is widely applicated in food industry, which shows high economical and nutritional values. However, growth of grapevine was usually affected by various environmental stresses, such as salt, drought and disease. Ubiquitin fusion degradation protein 1 (UFD1) is an essential ubiquitin-recognition protein facilitates regulation of stress response through ERAD pathway. Even though, a comprehensive investigation of UFD1 genes in the plant species is still lacking. Here we identified three VvUFD1 proteins from genome of grapevine, which were assigned into different subgroups. All VvUFD1 genes contain highly conserved motifs in structure. Several cis-elements that related to fruit development and stress response were found in the promoter regions of VvUFD1 genes, including bHLH, NCA, MYB, HD-ZIP, GATA and AP2. Expression analysis found VvUFD1 genes showed different expression patterns in different tissues. Most importantly, VvUFD1 genes were found to be involved in salt stress response during growth of grapevine. Transcriptomic analyses were investigated for further understanding the genes’ function. Expression of VvUFD1 were increased at late stage of berry ripening. In addition, expression of VvUFD1 were also regulated by elevated light treatment and pathogen Neofusicoccum parvum infection. Co-expression network analysis revealed several major transcription factors that co-expressed with VvUFD1 genes. These results provide a basis for investigating the function of UFD1 genes in plant species and expand understanding of the regulation of berry development and salt stress response in grapevine.

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The basic helix-loop-helix transcription factor bHLH95 affects fruit ripening and multiple metabolisms in tomato

Cited by 44 publications

References 55 publications

Transition to ripening in tomato requires hormone-controlled genetic reprogramming initiated in gel tissue

Transition to ripening in tomato requires hormone-controlled genetic reprogramming initiated in gel tissue

Transcriptome regulation of carotenoids in five flesh-colored watermelon (Citrullus lanatus)

Genome-wide identification and characterization of grapevine UFD1 genes during berry development and salt stress response

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